MERS-CoV Nucleocapsid Protein (His tag)
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- 抗原 See all MERS-CoV Nucleocapsid (MERS-CoV N) products
- MERS-CoV Nucleocapsid (MERS-CoV N) (MERS-Coronavirus Nucleocapsid Protein (MERS-CoV N))
- 蛋白类型
- Recombinant
- 宿主
- Middle East Respiratory Syndrome Coronavirus (MERS-CoV)
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资源
- Baculovirus infected Insect Cells
- 标记
- This MERS-CoV Nucleocapsid protein is labelled with His tag.
- 原理
- Recombinant MERS-CoV Nucleoprotein / NP protein (His Tag)
- 序列
- Met1-Asp413
- 产品特性
- A DNA sequence encoding the Nucleoprotein MERS-CoV (AFS88943.1) (Met1-Asp413) was fused with a polyhistidine tag at the C-terminus.
- 纯度
- > 90 % as determined by SDS-PAGE
- 内毒素水平
- < 1.0 EU per μg of the protein as determined by the LAL method
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MERS-Coronavirus Nucleocapsid Protein (MERS-CoV N) (AA 1-413) protein (His tag)
MERS-CoV N 宿主: Middle East Respiratory Syndrome Coronavirus (MERS-CoV) 宿主: HEK-293 Cells Recombinant >90 % as determined by SDS-PAGE.
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- 限制
- 仅限研究用
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- 状态
- Lyophilized
- 溶解方式
- Please refer to the printed manual for detailed information.
- 缓冲液
- Lyophilized from sterile 20 mM Tris, 500 mM NaCl, 10 % gly, pH 8.0. Normally 5 % - 8 % trehalose, mannitol and 0.01 % Tween80 are added as protectants before lyophilization. Please refer to the specific buffer information in the printed manual.
- 储存条件
- 4 °C,-20 °C,-80 °C
- 储存方法
- Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80°C. Reconstituted protein solution can be stored at 4-8°C for 2-7 days. Aliquots of reconstituted samples are stable at < -20°C for 3 months.
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- 抗原
- MERS-CoV Nucleocapsid (MERS-CoV N) (MERS-Coronavirus Nucleocapsid Protein (MERS-CoV N))
- 别名
- MERS-Coronavirus Nucleocapsid Protein (MERS-CoV N 产品)
- 背景
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Background: Coronaviruses are enveloped viruses with a positive-sense RNA genome and with a nucleocapsid of helical symmetry. Coronavirus nucleoproteins localize to the cytoplasm and the nucleolus, a subnuclear structure, in both virus-infected primary cells and in cells transfected with plasmids that express N protein. Coronavirus N protein is required for coronavirus RNA synthesis, and has RNA chaperone activity that may be involved in template switch. Nucleocapsid protein is a most abundant protein of coronavirus. During virion assembly, N protein binds to viral RNA and leads to formation of the helical nucleocapsid. Nucleocapsid protein is a highly immunogenic phosphoprotein also implicated in viral genome replication and in modulating cell signaling pathways. Because of the conservation of N protein sequence and its strong immunogenicity, the N protein of coronavirus is chosen as a diagnostic tool.
Synonym: coronavirus NP Protein, MERS-CoV, coronavirus Nucleocapsid Protein, MERS-CoV, coronavirus Nucleoprotein Protein, MERS-CoV, cov np Protein, MERS-CoV, ncov NP Protein, MERS-CoV, novel coronavirus Nucleoprotein Protein, MERS-CoV, NP Protein, MERS-CoV, Nucleocapsid Protein, MERS-CoV, Nucleoprotein Protein, MERS-CoV
- 分子量
- 46.51kDa.
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