VRK1 Protein (GST tag,His tag)
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- 抗原 See all VRK1 蛋白
- VRK1 (Vaccinia Related Kinase 1 (VRK1))
- 蛋白类型
- Recombinant
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宿主
- 人
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资源
- Baculovirus infected Insect Cells
- 标记
- This VRK1 protein is labelled with GST tag,His tag.
- 原理
- Recombinant Human VRK1 Protein (His & GST Tag)
- 序列
- Met 1-Lys 396
- 产品特性
- A DNA sequence encoding the human VRK1 (Q99986) (Met 1-Lys 396) was fused with the N-terminal polyhistidine-tagged GST tag at the N-terminus.
- 纯度
- > 88 % as determined by reducing SDS-PAGE.
- 内毒素水平
- < 1.0 EU per μg as determined by the LAL method.
- Top Product
- Discover our top product VRK1 蛋白
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Vaccinia Related Kinase 1 (VRK1) (AA 1-396) (Active) protein (GST tag)
VRK1 宿主: 人 宿主: 大肠杆菌（E. Coli） Recombinant SDS, Func Active
Vaccinia Related Kinase 1 (VRK1) (AA 1-396) (Active) protein (His-GST)VRK1 宿主: 人 宿主: Insect cells (Sf9) Recombinant SDS, Func Active
Vaccinia Related Kinase 1 (VRK1) (AA 1-396) protein (GST tag)VRK1 宿主: 人 宿主: 小麦胚 Recombinant WB, ELISA, AA, AP
Vaccinia Related Kinase 1 (VRK1) (AA 287-396) protein (GST tag)VRK1 宿主: 人 宿主: 小麦胚 Recombinant WB, ELISA, AA, AP
Vaccinia Related Kinase 1 (VRK1) (AA 1-396) protein (Strep Tag)Crystallography grade VRK1 宿主: 人 宿主: Tobacco (Nicotiana tabacum) Recombinant >80 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot. WB, SDS, ELISA
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- 限制
- 仅限研究用
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- 状态
- Lyophilized
- 溶解方式
- Please refer to the printed manual for detailed information.
- 缓冲液
- Lyophilized from sterile 20 mM Tris, 500 mM NaCl, pH 7.4, 10 % glycerol
- 储存条件
- 4 °C,-20 °C,-80 °C
- 储存方法
- Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80°C. Reconstituted protein solution can be stored at 4-8°C for 2-7 days. Aliquots of reconstituted samples are stable at < -20°C for 3 months.
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- 抗原
- VRK1 (Vaccinia Related Kinase 1 (VRK1))
- 别名
- VRK1 (VRK1 产品)
- 别名
- vrk1 Protein, MGC64390 Protein, MGC75762 Protein, VRK1 Protein, PCH1 Protein, PCH1A Protein, 51PK Protein, wu:fe16f06 Protein, zgc:56266 Protein, vaccinia related kinase 1 L homeolog Protein, vaccinia related kinase 1 Protein, Serine/threonine-protein kinase VRK1 Protein, vrk1.L Protein, vrk1 Protein, VRK1 Protein, vrk-1 Protein, Vrk1 Protein
- 背景
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Background: VRK1 is a member of the vaccinia-related kinase (VRK) family of serine/threonine protein kinases. Serine/threonine protein kinases are tumor suppressor that controls the activity of AMP-activated protein kinase family members, thereby playing a role in various processes such as cell metabolism, cell polarity, apoptosis and DNA damage response. VRK1 contains 1 protein kinase domain and localizes to the nucleus. VRK1 gene is widely expressed in human tissues and has increased expression in actively dividing cells, such as those in testis, thymus, fetal liver, and carcinomas. As a serine/threonine kinase, VRK1 phosphorylates 'Thr-18' of p53/TP53 and may thereby prevent the interaction between p53/TP53 and MDM2. Defects in VRK1 are the cause of pontocerebellar hypoplasia type 1 (PCH1), also called pontocerebellar hypoplasia with infantile spinal muscular atrophy or pontocerebellar hypoplasia with anterior horn cell disease. PCH1 is characterized by an abnormally small cerebellum and brainstem, central and peripheral motor dysfunction from birth, gliosis and anterior horn cell degeneration resembling infantile spinal muscular atrophy.
Synonym: PCH1,PCH1A
- 分子量
- 73 kDa
- UniProt
- Q99986
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