UBE2D4 Protein (His tag)
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- 抗原 See all UBE2D4 蛋白
- UBE2D4 (Ubiquitin-Conjugating Enzyme E2D 4 (UBE2D4))
- 蛋白类型
- Recombinant
- 宿主
- 人
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资源
- 大肠杆菌（E. Coli）
- 标记
- This UBE2D4 protein is labelled with His tag.
- 原理
- Recombinant Human UBE2D4 Protein (His Tag)
- 序列
- Met 1-Met 1474
- 产品特性
- A DNA sequence encoding the human UBE2D4 (Q9Y2X8) (Met 1-Met 1474) was expressed, with a polyhistide tag at the N-terminus.
- 纯度
- > 95 % as determined by reducing SDS-PAGE.
- Top Product
- Discover our top product UBE2D4 蛋白
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Ubiquitin-Conjugating Enzyme E2D 4 (UBE2D4) (AA 1-147) protein (His tag)
UBE2D4 宿主: 人 宿主: 大肠杆菌（E. Coli） Recombinant Greater than 95 % as determined by SDS-PAGE ELISA, WB
Ubiquitin-Conjugating Enzyme E2D 4 (UBE2D4) (AA 1-147) protein (GST tag)UBE2D4 宿主: 人 宿主: 小麦胚 Recombinant ELISA, WB, AA, AP
Ubiquitin-Conjugating Enzyme E2D 4 (UBE2D4) (AA 1-94) protein (GST tag)UBE2D4 宿主: 人 宿主: 小麦胚 Recombinant ELISA, WB, AA, AP
Ubiquitin-Conjugating Enzyme E2D 4 (UBE2D4) (AA 1-147) protein (Strep Tag)Crystallography grade UBE2D4 宿主: 人 宿主: Tobacco (Nicotiana tabacum) Recombinant >80 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot. ELISA, WB, SDS
Ubiquitin-Conjugating Enzyme E2D 4 (UBE2D4) protein (Myc-DYKDDDDK Tag)UBE2D4 宿主: 人 宿主: HEK-293 Cells Recombinant > 80 % as determined by SDS-PAGE and Coomassie blue staining AbP, STD
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- 限制
- 仅限研究用
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- 状态
- Lyophilized
- 溶解方式
- Please refer to the printed manual for detailed information.
- 缓冲液
- Lyophilized from sterile 20 mM Tris, 0.1M NaCl, 10 % glycerol, 2 mM DTT, pH 8.0
- 储存条件
- 4 °C,-20 °C,-80 °C
- 储存方法
- Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80°C. Reconstituted protein solution can be stored at 4-8°C for 2-7 days. Aliquots of reconstituted samples are stable at < -20°C for 3 months.
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- 抗原
- UBE2D4 (Ubiquitin-Conjugating Enzyme E2D 4 (UBE2D4))
- 别名
- UBE2D4 (UBE2D4 产品)
- 别名
- HBUCE1 Protein, UBE2D4 Protein, RGD69425 Protein, Ube2d2 Protein, Ube2d2b Protein, zgc:162263 Protein, ube2d3.2 Protein, EG546638 Protein, Gm5959 Protein, ubiquitin conjugating enzyme E2 D4 (putative) Protein, ubiquitin-conjugating enzyme E2D 4 (putative) Protein, ubiquitin-conjugating enzyme E2 D4 Protein, ubiquitin conjugating enzyme E2D 4 (putative) L homeolog Protein, ubiquitin-conjugating enzyme E2 D2 pseudogene Protein, ubiquitin-conjugating enzyme E2D 4 Protein, UBE2D4 Protein, Ube2d4 Protein, EDI_085450 Protein, EDI_185830 Protein, ube2d4 Protein, ube2d4.L Protein, LOC100354069 Protein
- 背景
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Background: UBE2D4 is a member of the ubiquitin-conjugating E2 family whose members perform the second step in the ubiquitination reaction. Initially identified as the main process for protein degradation; ubiquitination is believed nowadays to be crucial for a wider range of cellular processes. The outcome of the ubiquitin-conjugation reaction; and thereby the fate of the substrate; is heavily dependent on the number of ubiquitin molecules attached and how these ubiquitin molecules are inter-connected. To deal with this complexity and to allow adequate ubiquitination in time and space; a highly sophisticated conjugation machinery has been developed. In a sequential manner; ubiquitin becomes activated by an ubiquitin-activating enzyme (E1); which then transfers the ubiquitin to a group of ubiquitin-conjugating enzymes (E2s). Next; ubiquitin-loaded E2s are interacting with ubiquitin protein ligases (E3s) and ubiquitin is conjugated to substrates on recruitment by the E3. These three key enzymes are operating in a hierarchical system; wherein two E1s and 35 E2s have been found and hundreds of E3s have been identified in humans. It has been identified the UBE2D family (UBE2D1-4) as E2 partners for IDOL that support both autoubiquitination and IDOL-dependent ubiquitination of the LDLR in a cell-free system.
Synonym: Ubiquitin-Conjugating Enzyme E2 D4; HBUCE1; Ubiquitin Carrier Protein D4; Ubiquitin-Protein Ligase D4; UBE2D4; UBCH5D
- UniProt
- Q9Y2X8
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