Dnmt2 Protein (GST tag)
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- 抗原 See all Dnmt2 (TRDMT1) 蛋白
- Dnmt2 (TRDMT1) (tRNA Aspartic Acid Methyltransferase 1 (TRDMT1))
- 蛋白类型
- Recombinant
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宿主
- 人
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资源
- Baculovirus infected Insect Cells
- 标记
- This Dnmt2 protein is labelled with GST tag.
- 原理
- Recombinant Human DNMT2/TRDMT1 Protein (GST Tag)
- 序列
- Met 1-Glu 391
- 产品特性
- A DNA sequence encoding the human TRDMT1 isoform a (NP_004403.1) (Met 1-Glu 391) was fused with the GST tag at the N-terminus.
- 纯度
- > 94 % as determined by reducing SDS-PAGE.
- 内毒素水平
- < 1.0 EU per μg as determined by the LAL method.
- Top Product
- Discover our top product TRDMT1 蛋白
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tRNA Aspartic Acid Methyltransferase 1 (TRDMT1) (AA 1-391) protein (GST tag)
TRDMT1 宿主: 人 宿主: 小麦胚 Recombinant WB, ELISA, AA, AP
tRNA Aspartic Acid Methyltransferase 1 (TRDMT1) protein (His tag)TRDMT1 宿主: 人 宿主: 大肠杆菌（E. Coli） Recombinant > 80 % as determined by SDS-PAGE and Coomassie blue staining AbP, STD
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- 限制
- 仅限研究用
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- 状态
- Lyophilized
- 溶解方式
- Please refer to the printed manual for detailed information.
- 缓冲液
- Lyophilized from sterile 50 mM Tris, 100 mM NaCl, 0.5 mM GSH, 0.5 mM PMSF, pH 8.0
- 储存条件
- 4 °C,-20 °C,-80 °C
- 储存方法
- Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80°C. Reconstituted protein solution can be stored at 4-8°C for 2-7 days. Aliquots of reconstituted samples are stable at < -20°C for 3 months.
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- 抗原
- Dnmt2 (TRDMT1) (tRNA Aspartic Acid Methyltransferase 1 (TRDMT1))
- 别名
- DNMT2/TRDMT1 (TRDMT1 产品)
- 别名
- MGC89267 Protein, DMNT2 Protein, DNMT2 Protein, MHSAIIP Protein, PUMET Protein, RNMT1 Protein, Dnmt2 Protein, Rnmt2 Protein, dnmt2 Protein, tRNA aspartic acid methyltransferase 1 Protein, tRNA aspartic acid methyltransferase 1 L homeolog Protein, trdmt1 Protein, TRDMT1 Protein, Trdmt1 Protein, trdmt1.L Protein
- 背景
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Background: DNMT2, also known as tRNA (cytosine-5-)-methyltransferase, DNA methyltransferase homolog HsaIIP, and TRDMT1, is a member of the DNA methyltransferase family of enzymes. DNMT2 enzymes have been widely conserved during evolution and contain all of the signature motifs of DNA (cytosine-5)-methyltransferases. It contains all 10 sequence motifs that are conserved among m(5)C MTases, including the consensus S:-adenosyl-L-methionine-binding motifs and the active site ProCys dipeptide, and its structure is very similar to prokaryotic DNA methyltransferases. DNMT2 has close homologs in plants, insects and Schizosaccharomyces pombe, but no related sequence can be found in the genomes of Saccharomyces cerevisiae or Caenorhabditis elegans. While the biological function of DNMT2 is not yet known, the strong binding to DNA suggests that DNMT2 may mark specific sequences in the genome by binding to DNA through the specific target-recognizing motif. However, the DNA methyltransferase activity of these proteins is comparatively weak and their biochemical and functional properties remain enigmatic. Recent evidence now shows that Dnmt2 has a novel tRNA methyltransferase activity, raising the possibility that the biological roles of these proteins might be broader than previously thought.
Synonym: DMNT2,DNMT2,MHSAIIP,PUMET,RNMT1
- 分子量
- 71 kDa
- NCBI登录号
- NP_004403
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