TCN2 Protein (His tag)
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- 抗原 See all TCN2 蛋白
- TCN2 (Transcobalamin II (TCN2))
- 蛋白类型
- Recombinant
- 生物活性
- Active
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宿主
- 人
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资源
- HEK-293 Cells
- 标记
- This TCN2 protein is labelled with His tag.
- 原理
- Recombinant Human TCN2 Protein (His Tag)(Active)
- 序列
- Met 1-Trp 427
- 产品特性
- A DNA sequence encoding the human TCN2 (NP_000346.2) (Met 1-Trp 427) with a C-terminal polyhistidine tag was expressed.
- 纯度
- > 90 % as determined by reducing SDS-PAGE.
- 内毒素水平
- < 1.0 EU per μg as determined by the LAL method.
- Biological Activity Comment
- Measured by its binding ability in a functional ELISA. Immobilized human TCN2-His at 10μg/mL (100μL/well) can bind biotinylated mouse CD320-His.The EC50 of biotinylated mouse CD320-His is 18-42 ng/mL.
- Top Product
- Discover our top product TCN2 蛋白
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Transcobalamin II (TCN2) (AA 20-427) protein (GST tag)
TCN2 宿主: 人 宿主: 小麦胚 Recombinant WB, ELISA, AP, AA
Transcobalamin II (TCN2) (AA 1-400) protein (GST tag)TCN2 宿主: 人 宿主: 小麦胚 Recombinant WB, ELISA, AP, AA
Transcobalamin II (TCN2) protein (His tag)TCN2 宿主: 小鼠 宿主: HEK-293 Cells Recombinant > 95 % as determined by SDS-PAGE
Transcobalamin II (TCN2) (AA 19-427) protein (His tag)Crystallography grade TCN2 宿主: 人 宿主: Insect Cells Recombinant >95 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot. WB, SDS, ELISA, Crys
Transcobalamin II (TCN2) (AA 19-430) protein (His tag)Crystallography grade TCN2 宿主: 小鼠 宿主: Insect Cells Recombinant >95 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot. WB, SDS, ELISA, Crys
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- 限制
- 仅限研究用
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- 状态
- Lyophilized
- 溶解方式
- Please refer to the printed manual for detailed information.
- 缓冲液
- Lyophilized from sterile PBS, pH 7.4
- 储存条件
- 4 °C,-20 °C,-80 °C
- 储存方法
- Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80°C. Reconstituted protein solution can be stored at 4-8°C for 2-7 days. Aliquots of reconstituted samples are stable at < -20°C for 3 months.
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- 抗原
- TCN2 (Transcobalamin II (TCN2))
- 别名
- TCN2 (TCN2 产品)
- 别名
- AW208754 Protein, Tcn-2 Protein, Tc2 Protein, Tcn2p Protein, D22S676 Protein, D22S750 Protein, II Protein, TC Protein, TC II Protein, TC-2 Protein, TC2 Protein, TCII Protein, transcobalamin 2 Protein, Tcn2 Protein, TCN2 Protein
- 背景
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Background: Transcobalamin II, also known as TCN2 and TC II, is a plasma protein that binds cobalamin (Cbl, vitamin B12) as it is absorbed in the terminal ileum and distributes to tissues. The circulating transcobalamin II-cobalamin complex binds to receptors on the plasma membrane of tissue cells and is then internalized by receptor-mediated endocytosis. Transcobalamin II is a non-glycolated secretory protein of molecular mass 43 kDa. Its plasma membrane receptor (TC II-R) is a heavily glycosylated protein with a monomeric molecular mass of 62 kDa. Human TCN2 gene is composed of nine exons and eight introns spanning approximately 20 kb with multiple potential transcription start sites. A number of genetic abnormalities are characterized either by a failure to express TCN2 or by synthesis of an abnormal protein. The TCN2 deficiency results in cellular cobalamin deficiency, an early onset of megaloblastic anaemia, and neurological abnormalities.
Synonym: Transcobalamin-II,D22S676,D22S750,II,TC,TC-2,TC2,TCII
- 分子量
- 46.7 kDa
- NCBI登录号
- NP_000346
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