TFPI Protein (His tag)
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- 抗原 See all TFPI 蛋白
- TFPI (Tissue Factor Pathway Inhibitor (Lipoprotein-Associated Coagulation Inhibitor) (TFPI))
- 蛋白类型
- Recombinant
- 生物活性
- Active
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宿主
- 小鼠
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资源
- HEK-293 Cells
- 标记
- This TFPI protein is labelled with His tag.
- 原理
- Recombinant Mouse TFPI Protein (His Tag)(Active)
- 序列
- Met 1-Lys 289
- 产品特性
- A DNA sequence encoding the mouse TFPI isoform alpha (NP_035706.1) (Met 1-Lys 289) was expressed with a C-terminal polyhistidine tag.
- 纯度
- > 97 % as determined by SDS-PAGE
- 内毒素水平
- < 1.0 EU per μg of the protein as determined by the LAL method.
- Biological Activity Comment
- Measured by its ability to inhibit trypsin cleavage of a fluorogenic peptide substrate, Mca-RPKPVE-Nval-WRK(Dnp)-NH2 (Anaspec, Catalog # 27114). The IC50 value is < 0.5 nM, as measured in 100μL reaction mixture containing 1.25 ng trypsin (Sigma, Catalog # T4799), 10 μM substrate, 50 mM Tris, 10 mM CaCl2 , 0.15 M NaCl, 0.05% Brij 35, pH 7.5.
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- Discover our top product TFPI 蛋白
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Tissue Factor Pathway Inhibitor (Lipoprotein-Associated Coagulation Inhibitor) (TFPI) protein (His-Avi Tag)
TFPI 宿主: 人 宿主: HEK-293 Cells Recombinant > 95% as determined by Tris-Bis PAGE,> 95% as determined by HPLC
Tissue Factor Pathway Inhibitor (Lipoprotein-Associated Coagulation Inhibitor) (TFPI) (AA 1-251) protein (GST tag)TFPI 宿主: 人 宿主: 小麦胚 Recombinant WB, ELISA, AP, AA
Tissue Factor Pathway Inhibitor (Lipoprotein-Associated Coagulation Inhibitor) (TFPI) (AA 152-251) protein (GST tag)TFPI 宿主: 人 宿主: 小麦胚 Recombinant WB, ELISA, AP, AA
Tissue Factor Pathway Inhibitor (Lipoprotein-Associated Coagulation Inhibitor) (TFPI) protein (His-Avi Tag,Biotin)TFPI 宿主: 人 宿主: HEK-293 Cells Recombinant > 95% as determined by Tris-Bis PAGE
Tissue Factor Pathway Inhibitor (Lipoprotein-Associated Coagulation Inhibitor) (TFPI) (AA 29-304) protein (His tag)Crystallography grade TFPI 宿主: 人 宿主: Insect Cells Recombinant >95 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot. SDS, WB, ELISA, Crys
Tissue Factor Pathway Inhibitor (Lipoprotein-Associated Coagulation Inhibitor) (TFPI) (AA 29-306) protein (His tag)Crystallography grade TFPI 宿主: 小鼠 宿主: Insect Cells Recombinant >95 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot. SDS, WB, ELISA, Crys
Tissue Factor Pathway Inhibitor (Lipoprotein-Associated Coagulation Inhibitor) (TFPI) (AA 29-280), (partial) protein (GST tag)TFPI 宿主: 人 宿主: 大肠杆菌（E. Coli） Recombinant 90 % ELISA
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- 限制
- 仅限研究用
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- 状态
- Lyophilized
- 溶解方式
- Please refer to the printed manual for detailed information.
- 缓冲液
- Lyophilized from sterile PBS, pH 7.4
- 储存条件
- 4 °C,-20 °C,-80 °C
- 储存方法
- Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80°C. Reconstituted protein solution can be stored at 4-8°C for 2-7 days. Aliquots of reconstituted samples are stable at < -20°C for 3 months.
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- 抗原
- TFPI (Tissue Factor Pathway Inhibitor (Lipoprotein-Associated Coagulation Inhibitor) (TFPI))
- 别名
- TFPI (TFPI 产品)
- 别名
- EPI Protein, LACI Protein, TFI Protein, TFPI1 Protein, A630013F22Rik Protein, AW552122 Protein, TFPI Protein, si:dz44f8.1 Protein, tfpib Protein, wu:fb92g01 Protein, zgc:152844 Protein, zgc:158592 Protein, tissue factor pathway inhibitor Protein, tissue factor pathway inhibitor a Protein, TFPI Protein, Tfpi Protein, tfpia Protein
- 背景
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Background: Tissue factor pathway inhibitor (TFPI) is the natural inhibitor of TF coagulant and signaling activities. It is a Kunitz-type serine proteinase inhibitor that down-regulates tissue factor-initiated blood coagulation. With its Kunitz domains, TFPI exhibits significant homology with human inter-alpha-trypson inhibitor and bovin basic pancreatic trypsin inhibitor. TFPI is the natural inhibitor of TF coagulant and signaling activities. The importance of TFPI in the regulation of blood coagulation is emphasized by how its activity is modulated in human disease. In a factor (F) Xa-dependent feedback system, TFPI binds directly and inhibits the TF-FVII/FVIIa complex. Normally, TFPI exists in plasma both as a full-length molecule and as variably carboxy-terminal truncated forms. TFPI also circulates in complex with plasma lipoproteins. The levels and the dual inhibitor effect of TFPI on FXa and TF-FVII/FVIIa complex offers insight into the mechanisms of various pathological conditions triggered by TF. TFPI may play an important role in modulating TF-induced thrombogenesis and it may also provide a unique therapeutic approach for prophylaxis and/or treatment of various diseases. In addition, Studies have shown that TFPI exhibits antiangiogenic and antimetastatic effects in vitro and in vivo. In animal models of experimental metastasis, both circulating and tumor cell-associated TFPI are shown to significantly reduce tumor cell-induced coagulation activation and lung metastasis.
Synonym: A630013F22Rik,AW552122,EPI,LACI
- 分子量
- 31 kDa
- NCBI登录号
- NP_035706
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