SMPD1 Protein (AA 1-631) (His tag)
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- 抗原 See all SMPD1 蛋白
- SMPD1 (Sphingomyelin phosphodiesterase 1, Acid Lysosomal (SMPD1))
- 蛋白类型
- Recombinant
- 生物活性
- Active
- 产品特性
- AA 1-631
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宿主
- 人
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资源
- Baculovirus infected Insect Cells
- 标记
- This SMPD1 protein is labelled with His tag.
- 原理
- Recombinant Human SMPD1/ASM Protein (aa 1-631, His Tag)(Active)
- 序列
- Met 1-Cys 631
- 产品特性
- A DNA sequence encoding the full length of human SMPD1 isoform 1 (NP_000534.3) (Met 1-Cys 631) was expressed, fused with a polyhistidine tag at the C-terminus.
- 纯度
- > 94 % as determined by reducing SDS-PAGE.
- 内毒素水平
- < 1.0 EU per μg as determined by the LAL method.
- Biological Activity Comment
- Measured by its ability to cleave. 2-N-Hexadecanoylamino-4-nitrophenylphosphorylcholine (HNPPC). The specific activity is >1,000 pmol/min/μg.
- Top Product
- Discover our top product SMPD1 蛋白
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Sphingomyelin phosphodiesterase 1, Acid Lysosomal (SMPD1) (AA 531-630) protein (GST tag)
SMPD1 宿主: 人 宿主: 小麦胚 Recombinant WB, ELISA, AP, AA
Sphingomyelin phosphodiesterase 1, Acid Lysosomal (SMPD1) (AA 1-364) protein (GST tag)SMPD1 宿主: 人 宿主: 小麦胚 Recombinant WB, ELISA, AP, AA
Sphingomyelin phosphodiesterase 1, Acid Lysosomal (SMPD1) (Active) protein (His tag)SMPD1 宿主: 小鼠 宿主: Baculovirus infected Insect Cells Recombinant > 85 % as determined by SDS-PAGE Active
Sphingomyelin phosphodiesterase 1, Acid Lysosomal (SMPD1) protein (His tag)SMPD1 宿主: 人 宿主: Baculovirus infected Insect Cells Recombinant > 95 % as determined by reducing SDS-PAGE.
Sphingomyelin phosphodiesterase 1, Acid Lysosomal (SMPD1) (AA 47-629) protein (His tag)Crystallography grade SMPD1 宿主: 人 宿主: Insect Cells Recombinant >95 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot. WB, SDS, ELISA, Crys
Sphingomyelin phosphodiesterase 1, Acid Lysosomal (SMPD1) (AA 45-627) protein (His tag)Crystallography grade SMPD1 宿主: 小鼠 宿主: Insect Cells Recombinant >95 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot. WB, SDS, ELISA, Crys
Sphingomyelin phosphodiesterase 1, Acid Lysosomal (SMPD1) (Transcript Variant 1) protein (Myc-DYKDDDDK Tag)SMPD1 宿主: 人 宿主: HEK-293 Cells Recombinant > 80 % as determined by SDS-PAGE and Coomassie blue staining AbP, STD
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- 限制
- 仅限研究用
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- 状态
- Lyophilized
- 溶解方式
- Please refer to the printed manual for detailed information.
- 缓冲液
- Lyophilized from sterile 50 mM Tris, 100 mM NaCl, pH 8.0, 0.1 % OGP, 10 % glycerol
- 储存条件
- 4 °C,-20 °C,-80 °C
- 储存方法
- Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80°C. Reconstituted protein solution can be stored at 4-8°C for 2-7 days. Aliquots of reconstituted samples are stable at < -20°C for 3 months.
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- 抗原
- SMPD1 (Sphingomyelin phosphodiesterase 1, Acid Lysosomal (SMPD1))
- 别名
- SMPD1/ASM (SMPD1 产品)
- 别名
- ASM Protein, ASMASE Protein, NPD Protein, A-SMase Protein, Zn-SMase Protein, aSMase Protein, SMPD1 Protein, sphingomyelin phosphodiesterase 1 Protein, sphingomyelin phosphodiesterase 1, acid lysosomal Protein, sphingomyelin phosphodiesterase Protein, SMPD1 Protein, Smpd1 Protein, LOC5578088 Protein
- 背景
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Background: Sphingomyelin phosphodiesterase 1 (SMPD1) , also known as ASM ( acid sphingomyelinase ), is a member of the acid sphingomyelinase family of enzymes. Three isoforms have been identified, isoform 1 is 631 amino acids (aa) in length as the pro form, while Isoform 2 and isoform 3 have lost catalytic activity. The active SMPD1 isoform 1 contains one saposin B-type domain that likely interacts with sphingomyelin, and a catalytic region. Human SMPD1 is 86 % aa identical to mouse SMPD1. SMPD1 is a monomeric lysosomal enzyme that converts sphingomyelin (a plasma membrane lipid ) into ceramide through the removal of phosphorylcholine. This generates second messenger components that participate in signal transduction. Defects in SMPD1 are the cause of Niemann-Pick disease type A (NPA) and type B (NPB), also known as Niemann-Pick disease classical infantile form and Niemann-Pick disease visceral form. Niemann-Pick disease is a clinically and genetically heterogeneous recessive disorder. NPB has little if any neurologic involvement and patients may survive into adulthood.
Synonym: ASM,ASMASE,NPD
- 分子量
- 65 kDa
- NCBI登录号
- NP_000534
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