S100B Protein (Fc Tag)
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- 抗原 See all S100B 蛋白
- S100B (S100 Calcium Binding Protein B (S100B))
- 蛋白类型
- Recombinant
- 生物活性
- Active
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宿主
- 人
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资源
- HEK-293 Cells
- 标记
- This S100B protein is labelled with Fc Tag.
- 原理
- Recombinant Human S100B Protein (Fc Tag)(Active)
- 序列
- Ser 2-Glu 92
- 产品特性
- A DNA sequence encoding the human S100B (NP_006263.1) (Ser 2-Glu 92) was expressed with the fused Fc region of human IgG1 at the N-terminus.
- 纯度
- > 95 % as determined by reducing SDS-PAGE.
- 内毒素水平
- < 1.0 EU per μg as determined by the LAL method.
- Biological Activity Comment
- 1.Measured by its ability to bind mouse S100A1 in a functional ELISA.2. Measured by its ability to bind TP53 in a functional ELISA.3. Immobilized recombinant human Fc-S100B at 10 μg/mL (100 μl/well) can bind biotinylated human S100A1 with a linear range of 15.6-250 ng/mL.
- Top Product
- Discover our top product S100B 蛋白
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S100 Calcium Binding Protein B (S100B) (AA 2-92) protein (Fc Tag)
S100B 宿主: 犬 宿主: HEK-293 Cells Recombinant > 95 % as determined by Tris-Bis PAGE,> 95 % as determined by HPLC
S100 Calcium Binding Protein B (S100B) (AA 1-92) protein (His-IF2DI Tag)S100B 宿主: 人 宿主: 大肠杆菌（E. Coli） Recombinant Greater than 95 % by SDS-PAGE gel analyses WB, ELISA
S100 Calcium Binding Protein B (S100B) (AA 1-92) protein (GST tag)S100B 宿主: 人 宿主: 小麦胚 Recombinant WB, ELISA, AP, AA
S100 Calcium Binding Protein B (S100B) (Active) protein (Fc Tag)S100B 宿主: 小鼠 宿主: HEK-293 Cells Recombinant > 80 % as determined by SDS-PAGE Active
S100 Calcium Binding Protein B (S100B) (AA 1-92) (Active) protein (His tag)S100B 宿主: 小鼠 宿主: 大肠杆菌（E. Coli） > 90 % CC, AcA Active
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- 限制
- 仅限研究用
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- 状态
- Lyophilized
- 溶解方式
- Please refer to the printed manual for detailed information.
- 缓冲液
- Lyophilized from sterile PBS, pH 7.4
- 储存条件
- 4 °C,-20 °C,-80 °C
- 储存方法
- Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80°C. Reconstituted protein solution can be stored at 4-8°C for 2-7 days. Aliquots of reconstituted samples are stable at < -20°C for 3 months.
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- 抗原
- S100B (S100 Calcium Binding Protein B (S100B))
- 别名
- S100B (S100B 产品)
- 别名
- NEF Protein, S100 Protein, S100-B Protein, S100beta Protein, S100P Protein, AI850290 Protein, Bpb Protein, si:dkey-110p14.2 Protein, wu:fq18b10 Protein, zgc:92776 Protein, S100 calcium binding protein B Protein, S100 protein, beta polypeptide, neural Protein, S100 calcium binding protein, beta (neural) Protein, S100B Protein, S100b Protein, s100b Protein
- 背景
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Background: S100B is a member of the S100 family of proteins containing two EF-hand-type calcium-binding motifs. S100B exerts both intracellular and extracellular functions. Intracellular S100B acts as a stimulator of cell proliferation and migration and an inhibitor of apoptosis and differentiation, which might have important implications during brain, cartilage and skeletal muscle development and repair, activation of astrocytes in the course of brain damage and neurodegenerative processes, and of cardiomyocyte remodeling after infarction, as well as in melanomagenesis and gliomagenesis. As an extracellular factor, S100B engages RAGE (receptor for advanced glycation end products) in a variety of cell types with different outcomes (i.e. beneficial or detrimental, pro-proliferative or pro-differentiative) depending on the concentration attained by the protein, the cell type and the microenvironment. This calcium binding astrocyte-specific cytokine, presents a marker of astrocytic activation and reflects CNS injury. The excellent sensitivity of S100B has enabled it to confirm the existence of subtle brain injury in patients with mild head trauma, strokes, and after successful resuscitation from cardiopulmonary arrest. Recent findings provide evidence, that S100B may decrease neuronal injury and/or contribute to repair following traumatic brain injury (TBI). Hence, S100B, far from being a negative determinant of outcome, as suggested previously in the human TBI and ischemia literature, is of potential therapeutic value that could improve outcome in patients who sustain various forms of acute brain damage.
Synonym: Protein S100-B, S-100 protein beta chain, S-100 protein subunit beta, S100 calcium-binding protein B, S100b, S100 beta, S100 calcium binding protein B,NEF,S100,S100-B,S100beta
- 分子量
- 37.2 kDa
- NCBI登录号
- NP_006263
- 途径
- Regulation of Muscle Cell Differentiation, Positive Regulation of Immune Effector Process, Toll-Like Receptors Cascades, Regulation of long-term Neuronal Synaptic Plasticity, S100 Proteins
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