R-Spondin 3 Protein (RSPO3) (AA 1-146) (His tag)
-
- 抗原 See all R-Spondin 3 (RSPO3) 蛋白
- R-Spondin 3 (RSPO3)
- 蛋白类型
- Recombinant
- 生物活性
- Active
- 产品特性
- AA 1-146
-
宿主
- 人
-
资源
- HEK-293 Cells
- 标记
- This R-Spondin 3 protein is labelled with His tag.
- 原理
- Recombinant Human RSPO3 Protein (aa 1-146, His Tag)(Active)
- 序列
- Met 1-Val 146
- 产品特性
- A DNA sequence encoding the amino acids (Met 1-Val 146) of human RSPO3 (Q9BXY4-1) was fused with a polyhistidine tag at the C-terminus.
- 纯度
- > 96 % as determined by reducing SDS-PAGE.
- 内毒素水平
- < 1.0 EU per μg of the protein as determined by the LAL method.
- Biological Activity Comment
- Measured by its binding ability in a functional ELISA.2. Immobilized RSPO3-His (146) at 10 μg/mL (100 μL/well) can bind human RNF43-Fc .The EC50 of human RNF43-Fc is 0.01-0.03μg/mL.
- Top Product
- Discover our top product RSPO3 蛋白
-
R-Spondin 3 (RSPO3) (AA 24-272) protein (His-IF2DI Tag)
RSPO3 宿主: 人 宿主: 大肠杆菌（E. Coli） Recombinant Greater than 90 % by SDS-PAGE gel analyses WB, ELISA
R-Spondin 3 (RSPO3) (AA 22-272) protein (His tag)Crystallography grade RSPO3 宿主: 人 宿主: Insect Cells Recombinant >95 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot. WB, SDS, ELISA, Crys
R-Spondin 3 (RSPO3) (AA 22-277) protein (His tag)Crystallography grade RSPO3 宿主: 小鼠 宿主: Insect Cells Recombinant >95 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot. WB, SDS, ELISA, Crys
-
- 限制
- 仅限研究用
-
- 状态
- Lyophilized
- 溶解方式
- Please refer to the printed manual for detailed information.
- 缓冲液
- Lyophilized from sterile PBS, pH 7.4
- 储存条件
- 4 °C,-20 °C,-80 °C
- 储存方法
- Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80°C. Reconstituted protein solution can be stored at 4-8°C for 2-7 days. Aliquots of reconstituted samples are stable at < -20°C for 3 months.
-
- 抗原
- R-Spondin 3 (RSPO3)
- 别名
- RSPO3 (RSPO3 产品)
- 别名
- pwtsr Protein, thsd2 Protein, cristin1 Protein, CRISTIN1 Protein, PWTSR Protein, THSD2 Protein, RGD1563246 Protein, 2810459H04Rik Protein, AW742308 Protein, Cristin1 Protein, Thsd2 Protein, R-spondin 3 Protein, rspo3 Protein, RSPO3 Protein, Rspo3 Protein
- 背景
-
Background: R-spondin 3 (RSPO3) is a member of the R-Spondin (RSPO) family in vertebrates that activate Wnt/beta-catenin signaling, plays a key role in these processes. The RSPO family of secreted Wnt modulators is involved in development and disease and holds therapeutic promise as stem cell growth factors. The four members have high structural homology. RSPO2 and RSPO3 are more potent than RSPO1, whereas RSPO4 is relatively inactive. All RSPO members require Wnt ligands and LRP6 for activity and amplify signaling of Wnt3A, Wnt1, and Wnt7A, suggesting that RSPO proteins are general regulators of canonical Wnt signaling. RSPO3/PCP signaling during gastrulation requires Wnt5a and is transduced via Fz7, Dvl, and JNK. RSPO3 functions by inducing Sdc4-dependent, clathrin-mediated endocytosis. RSPO3 is a novel, evolutionarily conserved angiogenic factor in embryogenesis. RSPO3 has a key role in the interaction between chorion and allantois in labyrinthine development.
Synonym: R-spondin-3,RSPO3,Protein with TSP type-1 repeat, Roof plate-specific spondin-3, Thrombospondin type-1 domain-containing protein 2, PWTSR, THSD2, CRISTIN1
- 分子量
- 15.3 kDa
-
