RSPO2 Protein (Fc Tag)
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- 抗原 See all RSPO2 蛋白
- RSPO2 (R-Spondin 2 (RSPO2))
- 蛋白类型
- Recombinant
- 生物活性
- Active
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宿主
- 人
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资源
- HEK-293 Cells
- 标记
- This RSPO2 protein is labelled with Fc Tag.
- 原理
- Recombinant Human FTLS/RSPO2 Protein (186 Leu/Pro, Fc Tag)(Active)
- 序列
- Met 1-Gly 205, 186 Leu/Pro
- 产品特性
- A DNA sequence encoding the human RSPO2 (Q6UXX9-1) N-teminal fragment (Met 1-Gly 205, 186 Leu/Pro) was fused with the Fc region of human IgG1 at the C-terminus.
- 纯度
- > 90 % as determined by reducing SDS-PAGE.
- 内毒素水平
- < 1.0 EU per μg as determined by the LAL method.
- Biological Activity Comment
- Measured by its ability to induce activation of β-catenin response in a Topflash Luciferase assay using HEK293T human embryonic kidney cells.The ED50 for this effect is typically 10-60 ng/mL in the presence of 5 ng/mL recombinant mouse Wnt3a.
- Top Product
- Discover our top product RSPO2 蛋白
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R-Spondin 2 (RSPO2) (AA 1-176) protein (GST tag)
RSPO2 宿主: 人 宿主: 小麦胚 Recombinant ELISA, WB, AP, AA
R-Spondin 2 (RSPO2) (AA 22-243) protein (His tag)Crystallography grade RSPO2 宿主: 人 宿主: Insect Cells Recombinant >95 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot. ELISA, WB, Crys, SDS
R-Spondin 2 (RSPO2) (AA 24-243) protein (His tag)Crystallography grade RSPO2 宿主: 小鼠 宿主: Insect Cells Recombinant >95 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot. ELISA, WB, Crys, SDS
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- 限制
- 仅限研究用
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- 状态
- Lyophilized
- 溶解方式
- Please refer to the printed manual for detailed information.
- 缓冲液
- Lyophilized from sterile PBS, pH 7.4
- 储存条件
- 4 °C,-20 °C,-80 °C
- 储存方法
- Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80°C. Reconstituted protein solution can be stored at 4-8°C for 2-7 days. Aliquots of reconstituted samples are stable at < -20°C for 3 months.
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- 抗原
- RSPO2 (R-Spondin 2 (RSPO2))
- 别名
- FTLS/RSPO2 (RSPO2 产品)
- 别名
- RGD1562331 Protein, CRISTIN2 Protein, 2610028F08Rik Protein, AA673245 Protein, D430027K22 Protein, ftls Protein, R-spondin 2 Protein, R-spondin 2 L homeolog Protein, Rspo2 Protein, rspo2.L Protein, rspo2 Protein, RSPO2 Protein
- 背景
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Background: R-spondin-2, also known as RSPO2, synergizes with Wnt to activate beta-catenin. RSPO2 is secreted proteins that regulate beta-catenin signaling. Activator of the beta-catenin signaling cascade leads to TCF-dependent gene activation. Action both in the canonical Wnt / beta- catenin-dependent pathway, possibly via a direct interaction with Wnt proteins, and in a Wnt-independent beta catenin pathway through a receptor signaling pathway that may not use frizzled / LRP receptors. Probably also acts as a ligand for frizzled and LRP receptors. The encoding gene Rspo2 was identified as a novel common integration site for the mouse mammary tumor virus in viral induced mouse mammary tumors. Rspo2 and Rspo2 / Wnt1 tumors contained many spindle cells, consistent with an epithelial-mesenchymal transformation phenotype. When Rspo2 and Rspo2 / Wnt1 tumor cells were transferred into naive mice, they exhibited greater metastatic activity than cells derived from Wnt1 tumors.
Synonym: CRISTIN2
- 分子量
- 48.3 kDa
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