PDE4B Protein (GST tag,His tag)
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- 抗原 See all PDE4B 蛋白
- PDE4B (phosphodiesterase 4B, cAMP-Specific (PDE4B))
- 蛋白类型
- Recombinant
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宿主
- 人
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资源
- Baculovirus infected Insect Cells
- 标记
- This PDE4B protein is labelled with GST tag,His tag.
- 原理
- Recombinant Human PDE4B/DPDE4 Protein (His & GST Tag)
- 序列
- Met 1-Thr 564
- 产品特性
- A DNA sequence encoding the human PDE4B isofrom 2 (NP_001032416.1) (Met 1-Thr 564) was fused with the N-terminal polyhistidine-tagged GST tag at the N-terminus.
- 纯度
- > 80 % as determined by reducing SDS-PAGE.
- 内毒素水平
- < 1.0 EU per μg as determined by the LAL method.
- Top Product
- Discover our top product PDE4B 蛋白
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phosphodiesterase 4B, cAMP-Specific (PDE4B) (AA 637-736) protein (GST tag)
PDE4B 宿主: 人 宿主: 小麦胚 Recombinant WB, ELISA, AP, AA
phosphodiesterase 4B, cAMP-Specific (PDE4B) (AA 1-736) protein (Strep Tag)Crystallography grade PDE4B 宿主: 人 宿主: Tobacco (Nicotiana tabacum) Recombinant >80 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot. WB, SDS, ELISA
phosphodiesterase 4B, cAMP-Specific (PDE4B) (Transcript Variant D) protein (Myc-DYKDDDDK Tag)PDE4B 宿主: 人 宿主: HEK-293 Cells Recombinant > 80 % as determined by SDS-PAGE and Coomassie blue staining AbP, STD
phosphodiesterase 4B, cAMP-Specific (PDE4B) (Transcript Variant A) protein (Myc-DYKDDDDK Tag)PDE4B 宿主: 人 宿主: HEK-293 Cells Recombinant > 80 % as determined by SDS-PAGE and Coomassie blue staining AbP, STD
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- 限制
- 仅限研究用
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- 状态
- Lyophilized
- 溶解方式
- Please refer to the printed manual for detailed information.
- 缓冲液
- Lyophilized from sterile 50 mM Tris, 100 mM NaCl, 0.5 mM GSH, 10 % glycerol, 0.5 mM PMSF, pH 8.0
- 储存条件
- 4 °C,-20 °C,-80 °C
- 储存方法
- Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80°C. Reconstituted protein solution can be stored at 4-8°C for 2-7 days. Aliquots of reconstituted samples are stable at < -20°C for 3 months.
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- 抗原
- PDE4B (phosphodiesterase 4B, cAMP-Specific (PDE4B))
- 别名
- PDE4B/DPDE4 (PDE4B 产品)
- 别名
- dpde4 Protein, pde4b5 Protein, pdeivb Protein, Dpde4 Protein, R74983 Protein, dunce Protein, PDE4/IVb Protein, Pde4B1 Protein, Pde4B2 Protein, Pde4B3 Protein, Pde4B4 Protein, DPDE4 Protein, PDE4B5 Protein, PDEIVB Protein, phosphodiesterase 4B Protein, phosphodiesterase 4B, cAMP specific Protein, phosphodiesterase 4B S homeolog Protein, pde4b Protein, PDE4B Protein, Pde4b Protein, pde4b.S Protein
- 背景
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Background: cAMP-specific 3',5'-cyclic phosphodiesterase 4B, also known as PDE4B and DPDE4, is a member of the cyclic nucleotide phosphodiesterase family. PDE4 subfamily. Cyclic nucleotide phosphodiesterases (PDEs) comprise a large family of enzymes that catalyze the hydrolysis of cAMP or cGMP and are implicated in various diseases. The crystal structures reveal a common scheme of inhibitor binding to the PDEs: (i) a hydrophobic clamp formed by highly conserved hydrophobic residues that sandwich the inhibitor in the active site; (ii) hydrogen bonding to an invariant glutamine that controls the orientation of inhibitor binding. A scaffold can be readily identified for any given inhibitor based on the formation of these two types of conserved interactions. These structural insights will enable the design of isoform-selective inhibitors with improved binding affinity and should facilitate the discovery of more potent and selective PDE inhibitors for the treatment of a variety of diseases. PDE4B / DPDE4 hydrolyzes the second messenger cAMP, which is a key regulator of many important physiological processes. It is expressed in brain, heart, lung and skeletal muscle. PDE4B / DPDE4 may be involved in mediating central nervous system effects of therapeutic agents ranging from antidepressants to antiasthmatic and anti-inflammatory agents
Synonym: DPDE4;PDEIVB
- 分子量
- 92.2 kDa
- NCBI登录号
- NP_001032416
- 途径
- Cellular Response to Molecule of Bacterial Origin, cAMP Metabolic Process, Myometrial Relaxation and Contraction
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