NEK7 Protein (NEK7) (GST tag,His tag)
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- 抗原 See all NEK7 蛋白
- NEK7
- 蛋白类型
- Recombinant
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宿主
- 人
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资源
- Baculovirus infected Insect Cells
- 标记
- This NEK7 protein is labelled with GST tag,His tag.
- 原理
- Recombinant Human NEK7 Protein (His & GST Tag)
- 序列
- Met 1-Ser 302
- 产品特性
- A DNA sequence encoding the human NEK7 (NP_598001.1) (Met1-Ser 302) was fused with the N-terminal polyhistidine-tagged GST tag at the N-terminus.
- 纯度
- > 95 % as determined by reducing SDS-PAGE.
- 内毒素水平
- < 1.0 EU per μg as determined by the LAL method.
- Top Product
- Discover our top product NEK7 蛋白
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NEK7 (NEK7) (AA 1-302) (Active) protein (His-GST)
NEK7 宿主: 人 宿主: Insect cells (Sf9) Recombinant SDS, Func Active
NEK7 (NEK7) (AA 1-302) protein (His tag)NEK7 宿主: 人 宿主: 大肠杆菌（E. Coli） Recombinant Greater than 95 % as determined by SDS-PAGE ELISA, WB
NEK7 (NEK7) (AA 1-100) protein (GST tag)NEK7 宿主: 人 宿主: 小麦胚 Recombinant ELISA, WB, AP, AA
NEK7 (NEK7) (AA 1-302) protein (GST tag)NEK7 宿主: 人 宿主: 小麦胚 Recombinant ELISA, WB, AP, AA
NEK7 (NEK7) (AA 1-302) protein (Strep Tag)Crystallography grade NEK7 宿主: 小鼠 宿主: Tobacco (Nicotiana tabacum) Recombinant ≥ 80 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot. ELISA, WB, SDS
NEK7 (NEK7) (AA 1-302) protein (Strep Tag)Crystallography grade NEK7 宿主: 人 宿主: Tobacco (Nicotiana tabacum) Recombinant >80 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot. ELISA, WB, SDS
NEK7 (NEK7) protein (Myc-DYKDDDDK Tag)NEK7 宿主: 人 宿主: HEK-293 Cells Recombinant > 80 % as determined by SDS-PAGE and Coomassie blue staining AbP, STD
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- 限制
- 仅限研究用
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- 状态
- Lyophilized
- 溶解方式
- Please refer to the printed manual for detailed information.
- 缓冲液
- Lyophilized from sterile 50 mM Tris, 100 mM NaCl, pH 8.5, 0.5 mM Reduced Glutathione, 0.5 mM PMSF
- 储存条件
- 4 °C,-20 °C,-80 °C
- 储存方法
- Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80°C. Reconstituted protein solution can be stored at 4-8°C for 2-7 days. Aliquots of reconstituted samples are stable at < -20°C for 3 months.
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- 抗原
- NEK7
- 别名
- NEK7 (NEK7 产品)
- 别名
- 2810460C19Rik Protein, AU020186 Protein, wu:fj68f02 Protein, zgc:100962 Protein, zgc:92175 Protein, AtNek7 Protein, NIMA-related kinase 7 Protein, NIMA (never in mitosis gene a)-related expressed kinase 7 Protein, NIMA-related kinase 7 Protein, NIMA related kinase 7 Protein, NIMA-related kinase 7 S homeolog Protein, Nek7 Protein, nek7 Protein, NEK7 Protein, nek7.S Protein
- 背景
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Background: NIMA (never in mitosis gene a)-related kinase 7, NEK7 belongs to the NIMA subfamily, NEK Ser/Thr protein kinase family, protein kinase superfamily. NEKs (NIMA-related kinases) are mammalian serine/threonine (Ser/Thr) protein kinases structurally related to Aspergillus NIMA (Never in Mitosis, gene A), which plays essential roles in mitotic signaling. NEKs share an amino-terminal catalytic domain related to NIMA, an Aspergillus kinase involved in the control of several aspects of mitosis, and divergent carboxyl-terminal tails of varying length. NEKs are commonly referred to as mitotic kinases, although a definitive in vivo verification of this definition is largely missing. Reduction in the activity of NEK7 or its close paralog, NEK6, has previously been shown to arrest cells in mitosis, mainly at metaphase. NEK7 is a regulator of cell division, and reveal it as an essential component for mammalian growth and survival. The intimate connection between tetraploidy, aneuploidy and cancer development suggests that NEK7 deregulation can induce oncogenesis. The endogenous NEK7 protein is enriched at the centrosome in a microtubule-independent manner. Overexpression of wt or kinase-defective NEK7 resulted in cells of rounder appearance, and higher proportions of multinuclear and apoptotic cells.
Synonym: NEK7
- 分子量
- 62.4 kDa
- NCBI登录号
- NP_598001
- 途径
- Inflammasome
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