METAP2 Protein (His tag)
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- 抗原 See all METAP2 蛋白
- METAP2 (Methionyl Aminopeptidase 2 (METAP2))
- 蛋白类型
- Recombinant
- 生物活性
- Active
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宿主
- 小鼠
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资源
- Baculovirus infected Insect Cells
- 标记
- This METAP2 protein is labelled with His tag.
- 原理
- Recombinant Mouse METAP2 Protein (His Tag)(Active)
- 序列
- Ala 2-Tyr 478
- 产品特性
- A DNA sequence encoding the mouse METAP2 (O08663) (Ala 2-Tyr 478) was expressed,with a C-terminal polyhistidine tag.
- 纯度
- > 88 % as determined by SDS-PAGE
- 内毒素水平
- < 1.0 EU per μg of the protein as determined by the LAL method.
- Biological Activity Comment
- Measured by its ability to remove methionine from a fluorogenic peptide substrate H-Met-Gly-Pro-AMC (Catalog # ES017). The resulting GP-AMC is cleaved by Recombinant Human DPPIV/CD26 (Catalog # 1180-SE). The specific activity is >15 pmol/min/g
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Methionyl Aminopeptidase 2 (METAP2) (AA 2-478) protein (His tag)
Crystallography grade METAP2 宿主: 人 宿主: Insect Cells Recombinant >95 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot. SDS, WB, ELISA, Crys
Methionyl Aminopeptidase 2 (METAP2) (AA 2-478) protein (His tag)Crystallography grade METAP2 宿主: 小鼠 宿主: Insect Cells Recombinant >95 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot. SDS, WB, ELISA, Crys
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- 限制
- 仅限研究用
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- 状态
- Lyophilized
- 溶解方式
- Please refer to the printed manual for detailed information.
- 缓冲液
- Lyophilized from sterile 50 mM Tris, 100 mM NaCl, pH 8.0, 10 % glycerol
- 储存条件
- 4 °C,-20 °C,-80 °C
- 储存方法
- Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80°C. Reconstituted protein solution can be stored at 4-8°C for 2-7 days. Aliquots of reconstituted samples are stable at < -20°C for 3 months.
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- 抗原
- METAP2 (Methionyl Aminopeptidase 2 (METAP2))
- 别名
- METAP2 (METAP2 产品)
- 别名
- metap2 Protein, MGC53792 Protein, METAP2 Protein, GB13458 Protein, DDBDRAFT_0190923 Protein, DDBDRAFT_0304991 Protein, DDB_0190923 Protein, DDB_0304991 Protein, 4930584B20Rik Protein, A930035J23Rik Protein, AI047573 Protein, AL024412 Protein, AU014659 Protein, Amp2 Protein, Mnpep Protein, p67 Protein, p67eIF2 Protein, MAP2 Protein, MNPEP Protein, wu:fb98h06 Protein, zgc:66250 Protein, methionyl aminopeptidase 2 L homeolog Protein, methionyl aminopeptidase 2 Protein, methionine aminopeptidase 2 Protein, methionine aminopeptidase Protein, methionyl aminopeptidase 2 S homeolog Protein, methionyl aminopeptidase 2b Protein, metap2.L Protein, METAP2 Protein, metap2 Protein, LOC551771 Protein, CAALFM_C604080WA Protein, Metap2 Protein, metap2.S Protein, metap2b Protein
- 背景
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Background: METAP2 (Methionine aminopeptidase 2), also known as MAP2 is a a protein which belongs to the peptidase M24A family. MAP2 binds 2 cobalt or manganese ions and contains approximately 12 O-linked N-acetylglucosamine (GlcNAc) residues. It is found in all organisms and is especially important because of its critical role in tissue repair and protein degradation. The catalytic activity of human MAP2 toward Met-Val peptides is consistently two orders of magnitude higher than that of METAP1, suggesting that it is responsible for processing proteins containing N-terminal Met-Val and Met-Thr sequences in vivo. This protein functions both by protecting the alpha subunit of eukaryotic initiation factor 2 from inhibitory phosphorylation and by removing the amino-terminal methionine residue from nascent protein. MAP2 protects eukaryotic initiation factor EIF2S1 from translation-inhibiting phosphorylation by inhibitory kinases such as EIF2AK2/PKR and EIF2AK1/HCR. It also plays a critical role in the regulation of protein synthesis.
Synonym: 4930584B20Rik,A930035J23Rik,AI047573,AL024412,Amp2,AU014659,Mnpep,p67,p67eIF2
- 分子量
- 54.3 kDa
- UniProt
- O08663
- 途径
- Regulation of G-Protein Coupled Receptor Protein Signaling
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