MAD2L1 Protein (His tag)
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- 抗原 See all MAD2L1 蛋白
- MAD2L1 (MAD2 Mitotic Arrest Deficient-Like 1 (Yeast) (MAD2L1))
- 蛋白类型
- Recombinant
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宿主
- 人
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资源
- 大肠杆菌（E. Coli）
- 标记
- This MAD2L1 protein is labelled with His tag.
- 原理
- Recombinant Human MAD2L1/MAD2 Protein (His Tag)
- 序列
- Met 1-Asp 205
- 产品特性
- A DNA sequence encoding the human MAD2L1 (Q13257) (Met 1-Asp 205) was expressed, with a polyhistide tag at the N-terminus.
- 纯度
- > 96 % as determined by reducing SDS-PAGE.
- Top Product
- Discover our top product MAD2L1 蛋白
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MAD2 Mitotic Arrest Deficient-Like 1 (Yeast) (MAD2L1) (AA 1-205) protein (His tag)
MAD2L1 宿主: 人 宿主: 大肠杆菌（E. Coli） Recombinant Greater than 95 % as determined by SDS-PAGE ELISA, WB
MAD2 Mitotic Arrest Deficient-Like 1 (Yeast) (MAD2L1) (AA 1-205) protein (GST tag)MAD2L1 宿主: 人 宿主: 小麦胚 Recombinant ELISA, WB, AP, AA
MAD2 Mitotic Arrest Deficient-Like 1 (Yeast) (MAD2L1) (AA 2-205) protein (His tag)Crystallography grade MAD2L1 宿主: 人 宿主: 大肠杆菌（E. Coli） Recombinant >95 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot. SDS, ELISA, WB, Crys
MAD2 Mitotic Arrest Deficient-Like 1 (Yeast) (MAD2L1) (AA 2-205) protein (His tag)Crystallography grade MAD2L1 宿主: 小鼠 宿主: 大肠杆菌（E. Coli） Recombinant >95 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot. SDS, ELISA, WB, Crys
MAD2 Mitotic Arrest Deficient-Like 1 (Yeast) (MAD2L1) (AA 2-205) protein (His-SUMO Tag)MAD2L1 宿主: 人 宿主: 大肠杆菌（E. Coli） Recombinant > 90 % SDS
MAD2 Mitotic Arrest Deficient-Like 1 (Yeast) (MAD2L1) protein (Myc-DYKDDDDK Tag)MAD2L1 宿主: 人 宿主: HEK-293 Cells Recombinant > 80 % as determined by SDS-PAGE and Coomassie blue staining AbP, STD
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- 限制
- 仅限研究用
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- 状态
- Lyophilized
- 溶解方式
- Please refer to the printed manual for detailed information.
- 缓冲液
- Lyophilized from sterile PBS, 20 % glycerol, pH 7.4
- 储存条件
- 4 °C,-20 °C,-80 °C
- 储存方法
- Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80°C. Reconstituted protein solution can be stored at 4-8°C for 2-7 days. Aliquots of reconstituted samples are stable at < -20°C for 3 months.
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- 抗原
- MAD2L1 (MAD2 Mitotic Arrest Deficient-Like 1 (Yeast) (MAD2L1))
- 别名
- MAD2L1/MAD2 (MAD2L1 产品)
- 别名
- HSMAD2 Protein, MAD2 Protein, MXD2 Protein, MXI Protein, bHLHc11 Protein, AA673185 Protein, cb408 Protein, zgc:112133 Protein, mad2 Protein, XMad2 Protein, MAD2L1 Protein, mitotic arrest deficient 2 like 1 Protein, MAX interactor 1, dimerization protein Protein, MAD2 mitotic arrest deficient-like 1 Protein, MAD2 mitotic arrest deficient-like 1 (yeast) Protein, mitotic arrest deficient 2 like 1 S homeolog Protein, MAD2L1 Protein, MXI1 Protein, Mad2l1 Protein, mad2l1 Protein, mad2l1.S Protein
- 背景
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Background: Mitotic spindle assembly checkpoint protein MAD2A, also known as HsMAD2, Mitotic arrest deficient 2-like protein 1, MAD2-like protein 1, MAD2L1 and MAD2, is a nucleus and cytoplasm protein which belongs to the MAD2 family. MAD2L1 is a component of the spindle-assembly checkpoint that prevents the onset of anaphase until all chromosomes are properly aligned at the metaphase plate. MAD2L1 is required for the execution of the mitotic checkpoint which monitors the process of kinetochore-spindle attachment and inhibits the activity of the anaphase promoting complex by sequestering CDC20 until all chromosomes are aligned at the metaphase plate. MAD2L1 has two highly different native conformations, an inactive open conformation that cannot bind CDC20 and that predominates in cytosolic monomers, and an active closed conformation. MAD2L1 in the closed conformation preferentially dimerizes with another molecule in the open conformation, but can also form a dimer with a molecule in the closed conformation. Formation of a heterotetrameric core complex containing two molecules of MAD1L1 and of MAD2L1 in the closed conformation promotes binding of another molecule of MAD2L1 in the open conformation and the conversion of the open to the closed form, and thereby promotes interaction with CDC20.
Synonym: HSMAD2;MAD2
- 分子量
- 25.6 kDa
- UniProt
- Q13257
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