LRPAP1 Protein (His tag)
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- 抗原 See all LRPAP1 蛋白
- LRPAP1 (Low Density Lipoprotein Receptor-Related Protein Associated Protein 1 (LRPAP1))
- 蛋白类型
- Recombinant
- 生物活性
- Active
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宿主
- 人
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资源
- HEK-293 Cells
- 标记
- This LRPAP1 protein is labelled with His tag.
- 原理
- Recombinant Human LRPAP1/A2MRAP Protein (His Tag)(Active)
- 序列
- Tyr 35-Leu 357
- 产品特性
- A DNA sequence encoding the human LRPAP1 (NP_002328.1) (Tyr 35-Leu 357) was expressed, fused with a signal peptide at the N-terminus and a polyhistidine tag at the C-terminus.
- 纯度
- > 92 % as determined by reducing SDS-PAGE.
- 内毒素水平
- < 1.0 EU per μg as determined by the LAL method.
- Biological Activity Comment
- Measured by its binding ability in a functional ELISA. Immobilized human LRPAP1 at 0.5 μg/ml can bind human VLDLR with a range of 3.2-400 ng/ml.
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- Discover our top product LRPAP1 蛋白
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Low Density Lipoprotein Receptor-Related Protein Associated Protein 1 (LRPAP1) (AA 29-360) protein (His tag)
LRPAP1 宿主: 小鼠 宿主: 大肠杆菌（E. Coli） Recombinant Greater than 95 % as determined by SDS-PAGE WB, ELISA
Low Density Lipoprotein Receptor-Related Protein Associated Protein 1 (LRPAP1) (AA 35-357) protein (His tag)LRPAP1 宿主: 人 宿主: 大肠杆菌（E. Coli） Recombinant Greater than 95 % as determined by SDS-PAGE WB, ELISA
Low Density Lipoprotein Receptor-Related Protein Associated Protein 1 (LRPAP1) (AA 1-357) protein (GST tag)LRPAP1 宿主: 人 宿主: 小麦胚 Recombinant WB, ELISA, AP, AA
Low Density Lipoprotein Receptor-Related Protein Associated Protein 1 (LRPAP1) (Active) protein (His tag)LRPAP1 宿主: 小鼠 宿主: HEK-293 Cells Recombinant > 95 % as determined by SDS-PAGE Active
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- 限制
- 仅限研究用
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- 状态
- Lyophilized
- 溶解方式
- Please refer to the printed manual for detailed information.
- 缓冲液
- Lyophilized from sterile PBS, pH 7.4
- 储存条件
- 4 °C,-20 °C,-80 °C
- 储存方法
- Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80°C. Reconstituted protein solution can be stored at 4-8°C for 2-7 days. Aliquots of reconstituted samples are stable at < -20°C for 3 months.
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- 抗原
- LRPAP1 (Low Density Lipoprotein Receptor-Related Protein Associated Protein 1 (LRPAP1))
- 别名
- LRPAP1/A2MRAP (LRPAP1 产品)
- 别名
- wu:fi20f07 Protein, wu:fi22c06 Protein, LRPAP1 Protein, DKFZp459I2430 Protein, A2MRAP Protein, A2RAP Protein, HBP44 Protein, MRAP Protein, RAP Protein, AA617339 Protein, AI790446 Protein, AU042172 Protein, C77774 Protein, alpha-2-macroglobulin receptor-associated protein-like Protein, low density lipoprotein receptor-related protein associated protein 1 Protein, LDL receptor related protein associated protein 1 Protein, LDL receptor related protein associated protein 1 L homeolog Protein, LOC100231803 Protein, lrpap1 Protein, LRPAP1 Protein, Lrpap1 Protein, lrpap1.L Protein
- 背景
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Background: Receptor-associated protein (RAP) is a molecular chaperone for low density lipoprotein receptor-related protein (LRP), which plays a key role in cholesterol metabolism. The lipoprotein receptor-related protein (LRP) is an endocytic receptor for several ligands, such as alpha2-macroglobulin (alpha2 M) and apolipoprotein E. LRP is involved in the clearance of lipids from the bloodstream and is expressed in the atherosclerotic plaque. The LRP-associated protein (LRPAP in humans, RAP in mice) acts as a chaperone protein, stabilizing the nascent LRP peptide in the endoplasmic reticulum and Golgi complex. Alpha-2-macroglobulin receptor-associated protein, also known as low density lipoprotein receptor-related protein-associated protein 1, RAP and LRPAP1, is a 39 kDa protein and a member of the alpha-2-MRAP family. It is a receptor antagonist that interacts with several members of the low density lipoprotein (LDL) receptor gene family. Upon binding to these receptors, LRPAP1 inhibits all ligand interactions with the receptors. LRPAP1 is present on cell surface forming a complex with the alpha-2-macroglobulin receptor heavy and light chains. It binds with LRP1B and the binding is followed by internalization and degradation. LRPAP1 interacts with LRP1/alpha-2-macroglobulin receptor and LRP2 (previously called glycoprotein 330), and may be involved in the pathogenesis of membrane glomerular nephritis. LRPAP1 together with LRP2 forms the Heymann nephritis antigenic complex. LRP2 is expressed in epithelial cells of the thyroid, where it can serve as a receptor for the protein thyroglobulin. Intron 5 insertion/deletion polymorphism of RAP gene (LRPAP1) has been implicated in other diseases sharing etiology with gallstone disease (GSD). The LRPAP1 insertion/deletion polymorphism influences cholesterol homeostasis and may confer risk for gallstone disease and gallbladder carcinoma (GBC) incidence usually parallels with the prevalence of cholelithiosis. The genetic variations at the LRPAP1 locus may modulate Alzheimer disease (AD) phenotype beyond risk for disease. In addition, the variation at the LRPAP1 gene could contribute to the risk of developing an early episode of myocardial infarction (MI).
Synonym: A2MRAP,A2RAP,alpha-2-MRAP,HBP44,MRAP,MYP23,RAP
- 分子量
- 39.2 kDa
- NCBI登录号
- NP_002328
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