PLA2G7 Protein (His tag)
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- 抗原 See all PLA2G7 (Lp-PLA2) 蛋白
- PLA2G7 (Lp-PLA2) (Lipoprotein-Associated phospholipase A2 (Lp-PLA2))
- 蛋白类型
- Recombinant
- 生物活性
- Active
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宿主
- 人
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资源
- HEK-293 Cells
- 标记
- This PLA2G7 protein is labelled with His tag.
- 原理
- Recombinant Human PLA2G7/Lp-PLA2 Protein (His Tag)(Active)
- 序列
- Met 1-Asn 441
- 产品特性
- A DNA sequence encoding the human PLA2G7 (Q13093-1) precursor (Met 1-Asn 441) was expressed, with a C-terminal polyhistidine tag.
- 纯度
- > 88 % as determined by reducing SDS-PAGE.
- 内毒素水平
- < 1.0 EU per μg of the protein as determined by the LAL method.
- Biological Activity Comment
- Measured by its ability to cleave a colorimetric peptide substrate, 1O-hexadecyl-2-deoxy-2-thio Sacetylsnglyceryl-3-phosphoryl choline (2-Thio-PAF), in the presence of 5, 5'Dithiobis(2-nitrobenzoic acid) (DTNB).The specific activity is >5000 pmoles/min/μg.
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- Discover our top product Lp-PLA2 蛋白
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Lipoprotein-Associated phospholipase A2 (Lp-PLA2) (AA 22-440) protein (His tag)
Lp-PLA2 宿主: 小鼠 宿主: HEK-293 Cells Recombinant > 95 % as determined by Tris-Bis PAGE,> 95 % as determined by HPLC
Lipoprotein-Associated phospholipase A2 (Lp-PLA2) (AA 22-441) protein (His tag)Lp-PLA2 宿主: 人 宿主: HEK-293 Cells Recombinant > 95 % as determined by Tris-Bis PAGE
Lipoprotein-Associated phospholipase A2 (Lp-PLA2) (AA 22-441) protein (GST tag)Lp-PLA2 宿主: 人 宿主: 小麦胚 Recombinant WB, ELISA, AA, AP
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- 限制
- 仅限研究用
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- 状态
- Lyophilized
- 溶解方式
- Please refer to the printed manual for detailed information.
- 缓冲液
- Lyophilized from sterile 50 mM NaAc, 150 mM NaCl, 10 % glycerol, pH 5.0
- 储存条件
- 4 °C,-20 °C,-80 °C
- 储存方法
- Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80°C. Reconstituted protein solution can be stored at 4-8°C for 2-7 days. Aliquots of reconstituted samples are stable at < -20°C for 3 months.
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- 抗原
- PLA2G7 (Lp-PLA2) (Lipoprotein-Associated phospholipase A2 (Lp-PLA2))
- 别名
- PLA2G7/Lp-PLA2 (Lp-PLA2 产品)
- 别名
- LDL-PLA2 Protein, LP-PLA2 Protein, PAFAD Protein, PAFAH Protein, Lp-PLA2 Protein, PAF-AH Protein, R75400 Protein, zgc:77563 Protein, phospholipase A2 group VII Protein, phospholipase A2, group VII (platelet-activating factor acetylhydrolase, plasma) Protein, PLA2G7 Protein, Pla2g7 Protein, pla2g7 Protein
- 背景
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Background: Platelet-activating factor acetylhydrolase; also known as 1-alkyl-2-acetylglycerophosphocholine esterase; 2-acetyl-1-alkylglycero-phosphocholine esterase; Group-VIIA phospholipase A2; LDL-associated phospholipase A2; PAF 2-acylhydrolase; PLA2G7 and PAFAH; is secreted protein which belongs to the AB hydrolase superfamily and Lipase family. PLA2G7 / PAFAH modulates the action of platelet-activating factor (PAF) by hydrolyzing the sn-2 ester bond to yield the biologically inactive lyso-PAF. It has a specificity for substrates with a short residue at the sn-2 position. It is inactive against long-chain phospholipids. PLA2G7 / PAFAH is a potent pro- and anti-inflammatory molecule that has been implicated in multiple inflammatory disease processes; including cardiovascular disease. PLA2G7 also represents an important; potentially functional candidate in the pathophysiology of coronary artery disease (CAD). Defects in PLA2G7 are the cause of platelet-activating factor acetylhydrolase deficiency (PLA2G7 deficiency). It is a trait which is present in 27% of Japanese. It could have a significant physiologic effect in the presence of inflammatory bodily responses.
Synonym: 2-acetyl-1-alkylglycerophosphocholine esterase; EC 3.1.1; EC 3.1.1.47;1-alkyl-2-acetylglycerophosphocholine esterase; Group-VIIA phospholipase A2; gVIIA-PLA2; LDL-associated phospholipase A2; LDL-PLA(2); LDL-PLA2; lipoprotein-associated phospholipase A2; LpPLA2; Lp-PLA2; PAF acetylhydrolase; PAF-AH; PAFAHPAF 2-acylhydrolase; phospholipase A2; group VII (platelet-activating factor acetylhydrolase; PLA2G7; plasma); platelet-activating factor acetylhydrolase
- 分子量
- 49.2 kDa
- 途径
- Peptide Hormone Metabolism
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