KIRREL3 Protein (His tag)
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- 抗原 See all KIRREL3 蛋白
- KIRREL3 (Kin of IRRE Like 3 (KIRREL3))
- 蛋白类型
- Recombinant
- 生物活性
- Active
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宿主
- 大鼠
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资源
- HEK-293 Cells
- 标记
- This KIRREL3 protein is labelled with His tag.
- 原理
- Recombinant Rat KIRREL3/NEPH2 Protein (His Tag)(Active)
- 序列
- Met1-Ala523
- 产品特性
- A DNA sequence encoding the rat KIRREL3 (Q09GS6) (Met1-Ala523) was expressed with a polyhistidine tag at the C-terminus.
- 纯度
- > 95 % as determined by SDS-PAGE
- 内毒素水平
- < 1.0 EU per μg of the protein as determined by the LAL method
- Biological Activity Comment
- Measured by the ability of the immobilized protein to support the adhesion of MS1 mouse pancreatic islet endothelial cells. When cells are added to rat KIRREL3 coated plates (15 μg/mL, 100 μL/well), > 20% will adhere specifically after 90 minutes at 37 °C.
- Top Product
- Discover our top product KIRREL3 蛋白
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Kin of IRRE Like 3 (KIRREL3) (AA 1-778) protein (GST tag)
KIRREL3 宿主: 人 宿主: 小麦胚 Recombinant ELISA, WB, AP, AA
Kin of IRRE Like 3 (KIRREL3) (AA 679-778) protein (GST tag)KIRREL3 宿主: 人 宿主: 小麦胚 Recombinant ELISA, WB, AP, AA
Kin of IRRE Like 3 (KIRREL3) (Active) protein (His tag)KIRREL3 宿主: 小鼠 宿主: HEK-293 Cells Recombinant > 96 % as determined by SDS-PAGE Active
Kin of IRRE Like 3 (KIRREL3) protein (Myc-DYKDDDDK Tag)KIRREL3 宿主: 人 宿主: HEK-293 Cells Recombinant > 80 % as determined by SDS-PAGE and Coomassie blue staining AbP, STD
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- 限制
- 仅限研究用
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- 状态
- Lyophilized
- 溶解方式
- Please refer to the printed manual for detailed information.
- 缓冲液
- Lyophilized from sterile PBS, pH 7.4
- 储存条件
- 4 °C,-20 °C,-80 °C
- 储存方法
- Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80°C. Reconstituted protein solution can be stored at 4-8°C for 2-7 days. Aliquots of reconstituted samples are stable at < -20°C for 3 months.
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- 抗原
- KIRREL3 (Kin of IRRE Like 3 (KIRREL3))
- 别名
- KIRREL3/NEPH2 (KIRREL3 产品)
- 别名
- KIRRE Protein, MRD4 Protein, NEPH2 Protein, PRO4502 Protein, 1500010O20Rik Protein, 2900036G11Rik Protein, SST4 Protein, mKIAA1867 Protein, kirre like nephrin family adhesion molecule 3 Protein, kin of IRRE like 3 (Drosophila) Protein, KIRREL3 Protein, Kirrel3 Protein
- 背景
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Background: Kin of IRRE-like protein 3 (KIRREL3) also known as nin of irregular chiasm-like protein 3 or nephrin-like protein 2 (NEPH2) is a member of the nephrin-like protein family of transmembrane proteins, which includes NEPH1 (KIRREL) and NEPH3 (KIRREL2). KIRREL3/NEPH2 is expressed in fetalv and adult brain, and also in podocytes of kidney glomeruli. The cytoplasmic domains of KIRREL3/NEPH2 interact with the C-terminus of podocin, also expressed in the podocytes, cells involved in ensuring size- and charge-selective ultrafiltration. Mutations in KIRREL3/NEPH2 are associated with mental retardation autosomal dominant type 4. KIRREL3/NEPH2 expression is turned on in migrating nucleogenesis of the pontine nucleus (PN) neurons only after they enter the presumptive nuclear region. KIRREL3/NEPH2 knockdown disrupted the nuclear organization of PN presumably by changing the migratory behavior of PN neurons inside the nuclear region. Moreover, overexpression of the cytoplasmic region of KIRREL3, which can sequester intracellular signaling of endogenous KIRREL3, resulted in similar phenotypes. Overall, these results suggest KIRREL3 is involved in the nucleogenesis of the PN through the control of neuronal migration inside the nucleus.
Synonym: KIRREL3
- 分子量
- 56.4 kDa
- UniProt
- Q09GS6
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