JAM2 Protein (His tag)
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- 抗原 See all JAM2 蛋白
- JAM2 (Junctional Adhesion Molecule 2 (JAM2))
- 蛋白类型
- Recombinant
- 生物活性
- Active
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宿主
- 大鼠
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资源
- HEK-293 Cells
- 标记
- This JAM2 protein is labelled with His tag.
- 原理
- Recombinant Rat JAM-B/CD322 Protein (His Tag)(Active)
- 序列
- Met1-Asn236
- 产品特性
- A DNA sequence encoding the rat JAM2 (Q3MHC0) (Met1-Asn236) was expressed, fused with a polyhistidine tag at the C-terminus.
- 纯度
- > 90 % as determined by SDS-PAGE
- 内毒素水平
- < 1.0 EU per μg of the protein as determined by the LAL method
- Biological Activity Comment
- Measured by the ability of the immobilized protein to support the adhesion of Jurkat human acute T cell leukemia cells.When cells (8 x 104 cells/well) are added to rat JAM2-His coated plates (5 μg/mL, 100 μL/well), >30% will adhere after 60 minutes at 37°C.
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- Discover our top product JAM2 蛋白
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Junctional Adhesion Molecule 2 (JAM2) (AA 29-298) protein (GST tag)
JAM2 宿主: 人 宿主: 小麦胚 Recombinant WB, ELISA, AP, AA
Junctional Adhesion Molecule 2 (JAM2) (Active) protein (His tag)JAM2 宿主: 小鼠 宿主: HEK-293 Cells Recombinant > 97 % as determined by SDS-PAGE Active
Junctional Adhesion Molecule 2 (JAM2) protein (Fc Tag)JAM2 宿主: 大鼠 宿主: HEK-293 Cells Recombinant > 90 % as determined by SDS-PAGE
Junctional Adhesion Molecule 2 (JAM2) (AA 29-238) protein (His tag)Crystallography grade JAM2 宿主: 人 宿主: Insect Cells Recombinant >95 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot. SDS, WB, ELISA, Crys
Junctional Adhesion Molecule 2 (JAM2) (AA 29-236) protein (His tag)Crystallography grade JAM2 宿主: 小鼠 宿主: Insect Cells Recombinant >95 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot. SDS, WB, ELISA, Crys
Junctional Adhesion Molecule 2 (JAM2) (AA 29-298) protein (rho-1D4 tag)Crystallography grade JAM2 宿主: 人 宿主: Insect Cells Recombinant >95 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot. SDS, WB, ELISA, Crys
Junctional Adhesion Molecule 2 (JAM2) (AA 29-298) protein (rho-1D4 tag)Crystallography grade JAM2 宿主: 小鼠 宿主: Insect Cells Recombinant >95 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot. SDS, WB, ELISA, Crys
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- 限制
- 仅限研究用
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- 状态
- Lyophilized
- 溶解方式
- Please refer to the printed manual for detailed information.
- 缓冲液
- Lyophilized from sterile PBS, pH 7.4
- 储存条件
- 4 °C,-20 °C,-80 °C
- 储存方法
- Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80°C. Reconstituted protein solution can be stored at 4-8°C for 2-7 days. Aliquots of reconstituted samples are stable at < -20°C for 3 months.
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- 抗原
- JAM2 (Junctional Adhesion Molecule 2 (JAM2))
- 别名
- JAM-B/CD322 (JAM2 产品)
- 别名
- JAM2 Protein, cd322 Protein, jam-b Protein, jamb Protein, pro245 Protein, ve-jam Protein, vejam Protein, jam2 Protein, sb:cb1002 Protein, im:7155552 Protein, sc:d0813 Protein, si:ch211-160b11.5 Protein, LOC100227766 Protein, 1110002N23Rik Protein, 2410030G21Rik Protein, 2410167M24Rik Protein, JAM-2 Protein, JAM-B Protein, Jcam2 Protein, VE-JAM Protein, C21orf43 Protein, CD322 Protein, JAMB Protein, PRO245 Protein, VEJAM Protein, junctional adhesion molecule 2 Protein, junctional adhesion molecule 2a Protein, junctional adhesion molecule 2b Protein, junction adhesion molecule 2 Protein, JAM2 Protein, jam2 Protein, jam2a Protein, jam2b Protein, Jam2 Protein
- 背景
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Background: Junctional adhesion molecule B (JAM-B), also known as Junctional adhesion molecule 2 (JAM2), Vascular endothelial junction-associated molecule (VE-JAM), and CD322, is a single-pass type I membrane protein which belongs to the immunoglobulin superfamily. It is prominently expressed on high endothelial venules. expression to be restricted to the high endothelial venule of tonsil and lymph nodes. The localization to the endothelium of arterioles in and around inflammatory and tumor foci. JAM-B can function as an adhesive ligand for the T cell line J45 and can interact with GM-CSF/IL-4-derived peripheral blood dendritic cells, circulating CD56(+) NK cells, circulating CD56(+)CD3(+) NK/T cells, and circulating CD56(+)CD3(+)CD8(+) cytolytic T cells. JAM-2 is expressed on high endothelial venules (HEVs) in human tonsil and on a subset of human leukocytes, suggesting that JAM-2 plays a central role in the regulation of transendothelial migration. It binds to very late activation antigen (VLA)-4, a leucocyte integrin that contributes to rolling and firm adhesion of lymphocytes to endothelial cells through binding to vascular cell adhesion molecule (VCAM)-1. JAM-B appears to contribute to leucocyte extravasation by facilitating not only transmigration but also rolling and adhesion. JAM-B acts as an adhesive ligand for interacting with a variety of immune cell types and may play a role in lymphocyte homing to secondary lymphoid organs.
Synonym: JAM2,JAM-2
- 分子量
- 24.7 kDa
- UniProt
- Q3MHC0
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