IL1A Protein (AA 115-270) (His tag)
Our Local Distributor
北京 101111
Quick Overview for IL1A Protein (AA 115-270) (His tag) (ABIN7320583)
抗原
See all IL1A 蛋白蛋白类型
生物活性
宿主
资源
应用范围
纯度
-
-
产品特性
- AA 115-270
-
标记
- This IL1A protein is labelled with His tag.
-
原理
- Recombinant Mouse IL-1A/IL-1α Protein
-
序列
- Ser115-Ser270
-
产品特性
- Recombinant Mouse Interleukin-1 alpha is produced by our E.coli expression system and the target gene encoding Ser115-Ser270 is expressed.
-
过滤
- 0.2 μm filtered
-
内毒素水平
- < 0.1 EU per μg of the protein as determined by the LAL method.
-
Biological Activity Comment
- Measure by its ability to induce D10.G4.1 cells proliferation.The ED50 for this effect is <5 pg/mL.The specific activity of recombinant mouse IL-1 alpha is > 2 x 108 IU/mg.
-
-
-
-
应用备注
- Optimal working dilution should be determined by the investigator.
-
限制
- 仅限研究用
-
-
-
状态
- Lyophilized
-
缓冲液
-
Lyophilized from sterile PBS, pH 7.4.
Normally 5 % - 8 % trehalose, mannitol and 0.01 % Tween 80 are added as protectants before lyophilization. -
储存条件
- 4 °C,-20 °C,-80 °C
-
储存方法
- Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80°C. Reconstituted protein solution can be stored at 4-8°C for 2-7 days. Aliquots of reconstituted samples are stable at < -20°C for 3 months.
-
有效期
- 12 months
-
-
- IL1A (Interleukin 1 alpha (IL1A))
-
别名
- IL-1A/IL-1alpha
-
背景
- Hematopoietin,IL1F,Hematopoietin-1,IL1,IL1-A,IL1F1,IL-1α,Preinterleukin 1 Alpha,Pro-Interleukin-1-Alpha,Il-1a,Interleukin-1 alpha,IL-1 alpha,Il1a,Mouse Interleukin-1 (IL-1) designates two proteins, IL-1α and IL-1β, which are the products of distinct genes, but recognize the same cell surface receptors. IL-1α and IL-1β are structurally related polypeptides that show approximately 25 % homology at the amino acid level. Both proteins are produced by a wide variety of cells in response to stimuli such as those produced by inflammatory agents, infections, or microbial endotoxins. The proteins are synthesized as 31 kDa precursors that are subsequently cleaved into proteins with molecular weights of approximately 17.5 kDa.
-
分子量
-
Calculated MW: 18.9 kDa
Observed MW: 20 kDa
-
UniProt
- P01582
-
途径
- NF-kappaB Signaling, Autophagy, Cancer Immune Checkpoints
抗原
-