Insulin Protein (INS) (AA 25-53, AA 90-110)
This Recombinant Insulin protein is expressed in 酵母菌.
产品编号 ABIN7318503
发货至:
中国
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Contact Info
Our Local Distributor
中国
北京 101111
北京 101111
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Quick Overview for Insulin Protein (INS) (AA 25-53, AA 90-110) (ABIN7318503)
抗原
See all Insulin (INS) 蛋白
Insulin (INS)
蛋白类型
Recombinant
生物活性
Active
宿主
人
资源
酵母菌
应用范围
Cell Culture (CC)
纯度
> 95 % as determined by reducing SDS-PAGE.
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产品特性
- AA 25-53, AA 90-110
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原理
- Recombinant Human Insulin Protein
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序列
- Phe25-Lys53&Gly90-Asn110
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产品特性
- A DNA sequence encoding human insulin B chain (2LGB_B)(Phe1-Lys29) and human insulin A chain (2KXK_A)(Gly1-Asn21) was expressed with a linker AAK.
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过滤
- 0.2 μm filtered
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Biological Activity Comment
- Measured in a serum-free cell proliferation assay using MCF-7 human breast cancer cells. The ED50 for this effect is typically 0.2-1 μg/mL.
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Insulin (INS) protein
INS 宿主: 人 宿主: 酵母菌 Recombinant Greater than 95 % as determined by SDS-PAGE.
Insulin (INS) (AA 1-110) protein (GST tag)INS 宿主: 人 宿主: 小麦胚 Recombinant AP, AA, ELISA, WB
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应用备注
- Optimal working dilution should be determined by the investigator.
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限制
- 仅限研究用
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状态
- Lyophilized
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缓冲液
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Lyophilized from sterile PBS, pH 7.4.
Normally 5 % - 8 % trehalose, mannitol and 0.01 % Tween 80 are added as protectants before lyophilization. -
储存条件
- 4 °C,-20 °C,-80 °C
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储存方法
- Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80°C. Reconstituted protein solution can be stored at 4-8°C for 2-7 days. Aliquots of reconstituted samples are stable at < -20°C for 3 months.
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有效期
- 12 months
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- Insulin (INS)
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别名
- Insulin
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背景
- IDDM,IDDM1,IDDM2,ILPR,INS,IRDN,MODY10,After removal of the precursor signal peptide, proinsulin is post-translationally cleaved into three peptides: the B chain and A chain peptides, which are covalently linked via two disulfide bonds to form insulin, and C-peptide. Binding of insulin to the insulin receptor (INSR) stimulates glucose uptake.
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分子量
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Calculated MW: 7.1 kDa
Observed MW: 6 kDa
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基因ID
- 3630
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UniProt
- P01308
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途径
- NF-kappaB Signaling, RTK signaling, Positive Regulation of Peptide Hormone Secretion, Peptide Hormone Metabolism, Hormone Activity, Carbohydrate Homeostasis, ER-Nucleus Signaling, Regulation of Carbohydrate Metabolic Process, Feeding Behaviour, Autophagy, Negative Regulation of intrinsic apoptotic Signaling, Brown Fat Cell Differentiation, Positive Regulation of fat Cell Differentiation
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