IDS Protein (His tag)
-
- 抗原 See all IDS 蛋白
- IDS (Iduronate 2-Sulfatase (IDS))
- 蛋白类型
- Recombinant
- 生物活性
- Active
-
宿主
- 人
-
资源
- HEK-293 Cells
- 标记
- This IDS protein is labelled with His tag.
- 原理
- Recombinant Human Iduronate 2-Sulfatase/IDS Protein (His Tag)(Active)
- 序列
- Met 1-Pro 550
- 产品特性
- A DNA sequence encoding human IDS precursor (NP_000193.1) (Met 1-Pro 550) was expressed with a C-terminal polyhistidine tag.
- 纯度
- > 87 % as determined by reducing SDS-PAGE.
- 内毒素水平
- < 1.0 EU per μg as determined by the LAL method.
- Biological Activity Comment
- Measured by its ability to hydrolyze the substrate 4-Nitrocatechol Sulfate (PNCS).The specific activity is > 1.0 pmoles/min/μg.
- Top Product
- Discover our top product IDS 蛋白
-
Iduronate 2-Sulfatase (IDS) (AA 38-137) protein (GST tag)
IDS 宿主: 人 宿主: 小麦胚 Recombinant WB, ELISA, AP, AA
Iduronate 2-Sulfatase (IDS) (AA 1-312) protein (GST tag)IDS 宿主: 人 宿主: 小麦胚 Recombinant WB, ELISA, AP, AA
Iduronate 2-Sulfatase (IDS) (AA 26-550) protein (His tag)IDS 宿主: 人 宿主: Baculovirus infected Insect Cells Recombinant > 90 % by SDS - PAGE SDS
Iduronate 2-Sulfatase (IDS) proteinIDS 宿主: 小鼠 宿主: 大肠杆菌（E. Coli） Recombinant > 95 % WB, SDS, ELISA, IP
-
- 限制
- 仅限研究用
-
- 状态
- Lyophilized
- 溶解方式
- Please refer to the printed manual for detailed information.
- 缓冲液
- Lyophilized from sterile PBS, pH 7.4
- 储存条件
- 4 °C,-20 °C,-80 °C
- 储存方法
- Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80°C. Reconstituted protein solution can be stored at 4-8°C for 2-7 days. Aliquots of reconstituted samples are stable at < -20°C for 3 months.
-
- 抗原
- IDS (Iduronate 2-Sulfatase (IDS))
- 别名
- Iduronate 2-Sulfatase/IDS (IDS 产品)
- 别名
- mps2 Protein, sids Protein, zgc:158245 Protein, MPS2 Protein, SIDS Protein, AW214631 Protein, iduronate 2-sulfatase Protein, IDS Protein, CpipJ_CPIJ004938 Protein, ids Protein, Ids Protein
- 背景
-
Background: Iduronate 2-Sulfatase, also known as IDS, is a member of the highly conserved sulfatase family of enzymes that catalyze the hydrolysis of O- and N-sulfate esters from a variety of substrates. The human Iduronate 2-Sulfatase/IDS consists of a signal peptide, a pro peptide and a mature chain that may be further processed into two chains. Among the identified 18 human sulfatases, Iduronate 2-Sulfatase/IDS is required for the lysosomal degradation of the glycosaminoglycans (GAG), heparan sulfate and dermatan sulfate. Multiple mutations in this X-chromosome localized gene result in Iduronate 2-Sulfatase/IDS enzymatic deficiency, and lead to the sex-linked Mucopolysaccharidosis Type II (MPS II ), also known as Hunter Syndrome characterized by the lysosomal accumulation of the GAG and their excretion in urine. MPS II has a wide spectrum of clinical manifestations ranging from mild to severe due to the level of Iduronate 2-Sulfatase/IDS enzyme. Retroviral-mediated Iduronate 2-Sulfatase/IDS gene transfer into lymphoid cells would be a promising gene therapeutic strategy.
Synonym: IDS,MPS2,SIDS
- 分子量
- 61 kDa
- NCBI登录号
- NP_000193
- 途径
- Glycosaminoglycan Metabolic Process
-
