ENPEP Protein (His tag)
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- 抗原 See all ENPEP 蛋白
- ENPEP (Glutamyl Aminopeptidase (Aminopeptidase A) (ENPEP))
- 蛋白类型
- Recombinant
- 生物活性
- Active
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宿主
- 小鼠
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资源
- Baculovirus infected Insect Cells
- 标记
- This ENPEP protein is labelled with His tag.
- 原理
- Recombinant Mouse ENPEP/Aminopeptidase A Protein (His Tag)(Active)
- 序列
- Arg41-Pro945
- 产品特性
- A DNA sequence encoding the mature form of mouse ENPEP (P16406) (Arg41-Pro945) was expressed, with a polyhistidine tag at the N-terminus.
- 纯度
- > 91 % as determined by SDS-PAGE
- 内毒素水平
- < 1.0 EU per μg of the protein as determined by the LAL method.
- Biological Activity Comment
- Measured by its ability to cleave the fluorogenic peptide substrate, Glu-7-amido-4-methyl coumarin.The specific activity is > 200 pmoles/min/μg.
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Glutamyl Aminopeptidase (Aminopeptidase A) (ENPEP) (AA 41-945) (Active) protein (His tag)
ENPEP 宿主: 大鼠 宿主: Baculovirus infected Insect Cells Recombinant > 95 % as determined by SDS-PAGE Active
Glutamyl Aminopeptidase (Aminopeptidase A) (ENPEP) (AA 1-957) protein (Strep Tag)Crystallography grade ENPEP 宿主: 人 宿主: Tobacco (Nicotiana tabacum) Recombinant >80 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot. SDS, WB, ELISA
Glutamyl Aminopeptidase (Aminopeptidase A) (ENPEP) (AA 1-945) protein (Strep Tag)Crystallography grade ENPEP 宿主: 小鼠 宿主: Tobacco (Nicotiana tabacum) Recombinant ≥ 80 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot. SDS, WB, ELISA
Glutamyl Aminopeptidase (Aminopeptidase A) (ENPEP) (AA 40-957) protein (His tag)Crystallography grade ENPEP 宿主: 人 宿主: Insect Cells Recombinant >95 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot. SDS, WB, ELISA, Crys
Glutamyl Aminopeptidase (Aminopeptidase A) (ENPEP) (AA 40-945) protein (His tag)Crystallography grade ENPEP 宿主: 小鼠 宿主: Insect Cells Recombinant >95 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot. SDS, WB, ELISA, Crys
Glutamyl Aminopeptidase (Aminopeptidase A) (ENPEP) (AA 719-949) protein (His tag)ENPEP 宿主: 人 宿主: 大肠杆菌（E. Coli） Recombinant > 90 % SDS
Glutamyl Aminopeptidase (Aminopeptidase A) (ENPEP) protein (Myc-DYKDDDDK Tag)ENPEP 宿主: 人 宿主: HEK-293 Cells Recombinant > 80 % as determined by SDS-PAGE and Coomassie blue staining AbP, STD
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- 限制
- 仅限研究用
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- 状态
- Lyophilized
- 溶解方式
- Please refer to the printed manual for detailed information.
- 缓冲液
- Lyophilized from sterile 20 mM Tris, 500 mM NaCl, pH 7.4
- 储存条件
- 4 °C,-20 °C,-80 °C
- 储存方法
- Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80°C. Reconstituted protein solution can be stored at 4-8°C for 2-7 days. Aliquots of reconstituted samples are stable at < -20°C for 3 months.
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- 抗原
- ENPEP (Glutamyl Aminopeptidase (Aminopeptidase A) (ENPEP))
- 别名
- ENPEP/Aminopeptidase A (ENPEP 产品)
- 别名
- im:7152184 Protein, si:ch211-146m5.2 Protein, 6030431M22Rik Protein, APA Protein, Bp-1/6C3 Protein, Ly-51 Protein, Ly51 Protein, CD249 Protein, gp160 Protein, glutamyl aminopeptidase Protein, ENPEP Protein, enpep Protein, ECU01_1470 Protein, pepA Protein, LOC5568916 Protein, CpipJ_CPIJ011103 Protein, Eint_011390 Protein, Halhy_0222 Protein, Enpep Protein
- 背景
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Background: ENPEP, also known as aminopeptidase A, is a member of the peptidase M1 family. Members of this family are involved in response to cadmium ion and proteolysis. They located in 6 components and are expressed in 26 plant structures. ENPEP is expressed by epithelial cells of the proximal tubule cells and the glomerulus of the nephron. It also can be detected in a variety of other tissues. ENPEP probably plays a role in regulating growth and differentiation of early B-lineage cells. It also may play a role in the catabolic pathway of the renin-angiotensin system. ENPEP is a zinc-dependent membrane-bound aminopeptidase that catalyzes the cleavage of glutamatic and aspartatic amino acid residues from the N-terminus of polypeptides. It degrades vasoconstricting angiotensin II into angiotensin III and therefore helps to regulate blood pressure.
Synonym: 6030431M22Rik,APA,Bp-1/6C3,ENPEP,Ly-51,Ly51
- 分子量
- 106 kDa
- UniProt
- P16406
- 途径
- Peptide Hormone Metabolism, Regulation of Systemic Arterial Blood Pressure by Hormones
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