GBA3 Protein (His tag)
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- 抗原 See all GBA3 蛋白
- GBA3 (Glucosidase, Beta, Acid 3 (Cytosolic) (GBA3))
- 蛋白类型
- Recombinant
- 生物活性
- Active
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宿主
- 人
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资源
- Baculovirus infected Insect Cells
- 标记
- This GBA3 protein is labelled with His tag.
- 原理
- Recombinant Human GBA3/CBGL1 Protein (His Tag)(Active)
- 序列
- Met 1-Leu 469
- 产品特性
- A DNA sequence encoding the human GBA3 (NP_066024.1) (Met 1-Leu 469) was fused with a polyhistidine tag at the C-terminus.
- 纯度
- > 95 % as determined by reducing SDS-PAGE.
- 内毒素水平
- < 1.0 EU per μg as determined by the LAL method.
- Biological Activity Comment
- Measured by its ability to hydrolyze 4-methylumbelliferyl-β-D glucopyranoside. The specific activity is >1,500 pmoles/min/μg.
- Top Product
- Discover our top product GBA3 蛋白
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Glucosidase, Beta, Acid 3 (Cytosolic) (GBA3) (AA 402-468) protein (GST tag)
GBA3 宿主: 人 宿主: 小麦胚 Recombinant ELISA, WB, AA, AP
Glucosidase, Beta, Acid 3 (Cytosolic) (GBA3) (AA 1-469) protein (His tag)GBA3 宿主: 人 宿主: 大肠杆菌（E. Coli） Recombinant Greater than 95 % as determined by SDS-PAGE ELISA, WB
Glucosidase, Beta, Acid 3 (Cytosolic) (GBA3) (AA 1-469) protein (Strep Tag)Crystallography grade GBA3 宿主: 人 宿主: Tobacco (Nicotiana tabacum) Recombinant >80 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot. ELISA, WB, SDS
Glucosidase, Beta, Acid 3 (Cytosolic) (GBA3) (Transcript Variant 1) protein (Myc-DYKDDDDK Tag)GBA3 宿主: 人 宿主: HEK-293 Cells Recombinant > 80 % as determined by SDS-PAGE and Coomassie blue staining AbP, STD
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- 限制
- 仅限研究用
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- 状态
- Lyophilized
- 溶解方式
- Please refer to the printed manual for detailed information.
- 缓冲液
- Lyophilized from sterile 20 mM Tris, 500 mM NaCl, 10 % glycerol, pH 7.4
- 储存条件
- 4 °C,-20 °C,-80 °C
- 储存方法
- Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80°C. Reconstituted protein solution can be stored at 4-8°C for 2-7 days. Aliquots of reconstituted samples are stable at < -20°C for 3 months.
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- 抗原
- GBA3 (Glucosidase, Beta, Acid 3 (Cytosolic) (GBA3))
- 别名
- GBA3/CBGL1 (GBA3 产品)
- 别名
- im:6912508 Protein, CBG Protein, CBGL1 Protein, GLUC Protein, KLRP Protein, glucosylceramidase beta 3 Protein, glucosidase, beta, acid 3 (gene/pseudogene) Protein, glucosidase, beta, acid 3 (cytosolic) Protein, cytosolic beta-glucosidase Protein, glucosylceramidase beta 3 (gene/pseudogene) Protein, Gba3 Protein, gba3 Protein, GBA3 Protein, LOC100344458 Protein
- 背景
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Background: "Cytosolic beta-glucosidase, also known as Cytosolic beta-glucosidase-like protein 1, GBA3, CBG and CBGL1 is a cytoplasm protein which belongs to the glycosyl hydrolase 1 family and Klotho subfamily. GBA3 / CBGL1 is a glycosidase probably involved in the intestinal absorption and metabolism of dietary flavonoid glycosides. GBA3 / CBGL1 is present in small intestine (at protein level). GBA3 / CBGL1 is expressed in liver, small intestine, colon, spleen and kidney. GBA3 / CBGL1 is down-regulated in renal cell carcinomas and hepatocellular carcinomas. GBA3 / CBGL1 is able to hydrolyze a broad variety of glycosides including phytoestrogens, flavonols, flavones, flavanones and cyanogens. GBA3 / CBGL1 possesses beta-glycosylceramidase activity and may be involved in a nonlysosomal catabolic pathway of glycosylceramide.
Synonym: CBG;CBGL1;GBA3;GLUC;KLrP;MGC104276;MGC126878
- 分子量
- 55 kDa
- NCBI登录号
- NP_066024
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