GRK5 Protein (His tag)
-
- 抗原 See all GRK5 蛋白
- GRK5 (G Protein-Coupled Receptor Kinase 5 (GRK5))
- 蛋白类型
- Recombinant
-
宿主
- 人
-
资源
- Baculovirus infected Insect Cells
- 标记
- This GRK5 protein is labelled with His tag.
- 原理
- Recombinant Human GRK5/GPRK5 Protein (His Tag)
- 序列
- Met 1-Ser 590
- 产品特性
- A DNA sequence encoding the human GRK5 (NP_005299.1) (Met 1-Ser 590) was fused with a polyhistidine tag at the C-terminus.
- 纯度
- > 90 % as determined by reducing SDS-PAGE.
- 内毒素水平
- < 1.0 EU per μg as determined by the LAL method.
- Top Product
- Discover our top product GRK5 蛋白
-
G Protein-Coupled Receptor Kinase 5 (GRK5) (AA 51-150) protein (GST tag)
GRK5 宿主: 人 宿主: 小麦胚 Recombinant WB, ELISA, AA, AP
G Protein-Coupled Receptor Kinase 5 (GRK5) protein (Myc-DYKDDDDK Tag)GRK5 宿主: 人 宿主: HEK-293 Cells Recombinant > 80 % as determined by SDS-PAGE and Coomassie blue staining AbP, STD
-
- 限制
- 仅限研究用
-
- 状态
- Lyophilized
- 溶解方式
- Please refer to the printed manual for detailed information.
- 缓冲液
- Lyophilized from sterile 50 mM Tris, 100 mM NaCl, 0.5 mM PMSF, 1 mM DTT, 0.5 mM EDTA, 10 % glycerol, pH 7.4
- 储存条件
- 4 °C,-20 °C,-80 °C
- 储存方法
- Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80°C. Reconstituted protein solution can be stored at 4-8°C for 2-7 days. Aliquots of reconstituted samples are stable at < -20°C for 3 months.
-
- 抗原
- GRK5 (G Protein-Coupled Receptor Kinase 5 (GRK5))
- 别名
- GRK5/GPRK5 (GRK5 产品)
- 别名
- GPRK5 Protein, Gprk5 Protein, si:dkey-171l20.1 Protein, GRK5 Protein, DKFZp468J1119 Protein, grk5 Protein, G protein-coupled receptor kinase 5 Protein, G protein-coupled receptor kinase 5 L homeolog Protein, GRK5 Protein, Grk5 Protein, grk5 Protein, grk5.L Protein
- 背景
-
Background: G protein-coupled receptor kinase 5, also known as G protein-coupled receptor kinase GRK5 and GRK5, is a member of the protein kinase superfamily, AGC Ser/Thr protein kinase family and GPRK subfamily. GRKs specifically phosphorylate agonist-occupied G protein-coupled receptors at the inner surface of the plasma membrane (PM), leading to receptor desensitization. GRKs utilize a variety of mechanisms to bind tightly, and sometimes reversibly, to cellular membranes. GRKs play an important role in mediating agonist-specific desensitization of numerous G protein-coupled receptors. GRK5 contains one AGC-kinase C-terminal domain, one protein kinase domain and one RGS domain. GRK5 specifically phosphorylates the activated forms of G protein-coupled receptors. Phospholipid-stimulated autophosphorylation may represent a novel mechanism for membrane association and regulation of GRK5 activity. GRK5 deficiency significantly exaggerates microgliosis and astrogliosis in the presence of an inflammatory initiator, such as the excess fibrillar Abeta and the subsequent active inflammatory reactions. GRK5 deficiency has been linked to early Alzheimer's disease in humans and mouse models of the disease.
Synonym: GPRK5
- 分子量
- 69 kDa
- NCBI登录号
- NP_005299
- 途径
- Myometrial Relaxation and Contraction, Regulation of G-Protein Coupled Receptor Protein Signaling
-
