AGO1 Protein (His tag)
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- 抗原 See all AGO1 (EIF2C1) 蛋白
- AGO1 (EIF2C1) (Eukaryotic Translation Initiation Factor 2C, 1 (EIF2C1))
- 蛋白类型
- Recombinant
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宿主
- 人
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资源
- Baculovirus infected Insect Cells
- 标记
- This AGO1 protein is labelled with His tag.
- 原理
- Recombinant Human AGO1/Argonaute 1/EIF2C1 Protein (His Tag)
- 序列
- Met 1-Ala 857
- 产品特性
- A DNA sequence encoding the full length of human AGO1 (NP_036331.1) (Met 1-Ala 857) was expressed, with a polyhistidine tag at the N-terminus.
- 纯度
- > 97 % as determined by reducing SDS-PAGE.
- 内毒素水平
- < 1.0 EU per μg as determined by the LAL method.
- Top Product
- Discover our top product EIF2C1 蛋白
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Eukaryotic Translation Initiation Factor 2C, 1 (EIF2C1) (AA 705-857) protein (His-IF2DI Tag)
EIF2C1 宿主: 人 宿主: 大肠杆菌（E. Coli） Recombinant Greater than 95 % by SDS-PAGE gel analyses WB, ELISA
Eukaryotic Translation Initiation Factor 2C, 1 (EIF2C1) (AA 101-200) protein (GST tag)EIF2C1 宿主: 人 宿主: 小麦胚 Recombinant WB, ELISA, AA, AP
Eukaryotic Translation Initiation Factor 2C, 1 (EIF2C1) (AA 1-857) protein (GST tag)EIF2C1 宿主: 人 宿主: 小麦胚 Recombinant WB, ELISA, AA, AP
Eukaryotic Translation Initiation Factor 2C, 1 (EIF2C1) (AA 1-857) protein (Strep Tag)Crystallography grade EIF2C1 宿主: 人 宿主: Tobacco (Nicotiana tabacum) Recombinant >80 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot. WB, ELISA, SDS
Eukaryotic Translation Initiation Factor 2C, 1 (EIF2C1) (AA 1-857) protein (Strep Tag)Crystallography grade EIF2C1 宿主: 小鼠 宿主: Tobacco (Nicotiana tabacum) Recombinant ≥ 80 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot. WB, ELISA, SDS
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- 限制
- 仅限研究用
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- 状态
- Lyophilized
- 溶解方式
- Please refer to the printed manual for detailed information.
- 缓冲液
- Lyophilized from sterile 50 mM Tris, 100 mM NaCl, 10 % glycerol, 0.5 PMSF, 0.5 mM EDTA, pH 8.0
- 储存条件
- 4 °C,-20 °C,-80 °C
- 储存方法
- Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80°C. Reconstituted protein solution can be stored at 4-8°C for 2-7 days. Aliquots of reconstituted samples are stable at < -20°C for 3 months.
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- 抗原
- AGO1 (EIF2C1) (Eukaryotic Translation Initiation Factor 2C, 1 (EIF2C1))
- 别名
- AGO1/Argonaute 1/EIF2C1 (EIF2C1 产品)
- 别名
- Ago Protein, Ago-1 Protein, Ago1 Protein, CG6671 Protein, Dm Ago1 Protein, Dmel\\CG6671 Protein, MRE20 Protein, ago1 Protein, ago1-1 Protein, anon-WO0257455.29 Protein, dAGO1 Protein, dAgo1 Protein, l(2)04845 Protein, l(2)4845 Protein, l(2)k00208 Protein, l(2)k08121 Protein, GB12654 Protein, dsim_GLEANR_9718 Protein, DsimGD25729 Protein, GD25729 Protein, EIF2C1 Protein, EIF2C Protein, GERP95 Protein, Q99 Protein, Eif2c1 Protein, ARGONAUTE 1 Protein, T1N15.2 Protein, T1N15_2 Protein, Argonaute-1 Protein, protein argonaute-2 Protein, argonaute 1 Protein, Argonaute 1 Protein, protein argonaute-1 Protein, argonaute 1, RISC catalytic component Protein, argonaute RISC catalytic subunit 1 Protein, Stabilizer of iron transporter SufD / Polynucleotidyl transferase Protein, AGO1 Protein, LOC552062 Protein, ago1 Protein, LOC659936 Protein, Dsim\AGO1 Protein, Ago1 Protein, LOC100386910 Protein, LOC100482671 Protein, LOC475337 Protein
- 背景
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Background: Protein argonaute-1, also known as eukaryotic translation initiation factor 2C 1, EIF2C1, and AGO1, is a member of the argonaute family and ago subfamily. Protein argonaute-1 in humans is encoded by the EIF2C1 gene. This gene is located on chromosome 1 in a cluster of closely related family members including argonaute 3, and argonaute 4. This genomic region is frequently lost in human cancers such as Wilms tumors, neuroblastoma, and carcinomas of the breast, liver, and colon. The human EIF2C1 gene is ubiquitously expressed at low to medium levels. Differential polyadenylation and splicing result in a complex transcriptional pattern. EIF2C1 protein contains one PAZ domain and one Piwi domain. It is required for RNA-mediated gene silencing (RNAi) and transcriptional gene silencing (TGS) of promoter regions which are complementary to bound short antigene RNAs (agRNAs). EIF2C1 binds to short RNAs such as microRNAs (miRNAs) or short interfering RNAs (siRNAs), and represses the translation of mRNAs which are complementary to them.
Synonym: EIF2C;EIF2C1;GERP95;Q99
- 分子量
- 99.5 kDa
- NCBI登录号
- NP_036331
- 途径
- Fc-epsilon Receptor Signaling Pathway, Regulatory RNA Pathways, EGFR Signaling Pathway, Neurotrophin Signaling Pathway, Hormone Transport, Regulation of Actin Filament Polymerization, Stem Cell Maintenance, Ribonucleoprotein Complex Subunit Organization
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