EPOR Protein (His tag)
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- 抗原 See all EPOR 蛋白
- EPOR (Erythropoietin Receptor (EPOR))
- 蛋白类型
- Recombinant
- 生物活性
- Active
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宿主
- 小鼠
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资源
- HEK-293 Cells
- 标记
- This EPOR protein is labelled with His tag.
- 原理
- Recombinant Mouse EPO Receptor/EPOR Protein (His Tag)(Active)
- 序列
- Met 1-Pro 249
- 产品特性
- A DNA sequence encoding the extracellular domain of mouse EPOR (NP_034279.3) (Met 1-Pro 249) was expressed, with a C-terminal polyhistidine tag.
- 纯度
- > 95 % as determined by SDS-PAGE
- 内毒素水平
- < 1.0 EU per μg of the protein as determined by the LAL method.
- Biological Activity Comment
- 1. Measured by its ability to inhibit EPO-dependent proliferation of TF-1 human erythroleukemic cells.The ED50 for this effect is typically 0.1-0.5 μg/mL in the presence of 16 ng/mL Recombinant mouse EPO.2. Measured by its binding ability in a functional ELISA.3. Immobilized mouse EPOR-His at 10μg/mL (100μL/well) can bind biotinylated mouse EPO-His.The EC50 of biotinylated mouse EPO-His is 34.5-80.6ng/mL.
- Top Product
- Discover our top product EPOR 蛋白
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Erythropoietin Receptor (EPOR) (AA 25-138) protein (His-IF2DI Tag)
EPOR 宿主: 人 宿主: 大肠杆菌（E. Coli） Recombinant Greater than 95 % by SDS-PAGE gel analyses WB, ELISA
Erythropoietin Receptor (EPOR) (AA 1-508) protein (GST tag)EPOR 宿主: 人 宿主: 小麦胚 Recombinant WB, ELISA, AA, AP
Erythropoietin Receptor (EPOR) (AA 31-130) protein (GST tag)EPOR 宿主: 人 宿主: 小麦胚 Recombinant WB, ELISA, AA, AP
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- 限制
- 仅限研究用
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- 状态
- Lyophilized
- 溶解方式
- Please refer to the printed manual for detailed information.
- 缓冲液
- Lyophilized from sterile PBS, pH 7.4
- 储存条件
- 4 °C,-20 °C,-80 °C
- 储存方法
- Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80°C. Reconstituted protein solution can be stored at 4-8°C for 2-7 days. Aliquots of reconstituted samples are stable at < -20°C for 3 months.
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- 抗原
- EPOR (Erythropoietin Receptor (EPOR))
- 别名
- EPO Receptor/EPOR (EPOR 产品)
- 别名
- EPOR Protein, epor Protein, EPO-R Protein, erythropoietin receptor Protein, erythropoietin receptor L homeolog Protein, EPOR Protein, epor Protein, Epor Protein, epor.L Protein
- 背景
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Background: Erythropoietin (EPO) is the major glycoprotein hormone regulator of mammalian erythropoiesis, and is produced by kidney and liver in an oxygen-dependent manner. The biological effects of EPO are mediated by the specific erythropoietin receptor (EPOR/EPO Receptor) on bone marrow erythroblasts, which transmits signals important for both proliferation and differentiation along the erythroid lineage. EPOR protein is a type â… single-transmembrane cytokine receptor, and belongs to the homodimerizing subclass which functions as ligand-induced or ligand-stabilized homodimers. EPOR signaling prevents neuronal death and ischemic injury. Recent studies have shown that EPO and EPOR protein may be involved in carcinogenesis, angiogenesis, and invasion.
Synonym: Epor
- 分子量
- 26.2 kDa
- NCBI登录号
- NP_034279
- 途径
- JAK/STAT Signaling
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