EGF 蛋白
Quick Overview for EGF 蛋白 (ABIN7195584)
抗原
See all EGF 蛋白蛋白类型
生物活性
宿主
资源
纯度
-
-
原理
- Recombinant Rat EGF Protein
-
序列
- Asn974-Arg1026
-
产品特性
- A DNA sequence encoding the rat EGF (NP_036974.1) (Asn974-Arg1026) was expressed and purified with an initial Met.
-
Biological Activity Comment
- Measured in a cell proliferation assay using BALB/c 3T3 mouse fibroblasts. The ED50 for this effect is typically 0.05-0.3ng/mL.
-
-
Epidermal Growth Factor (EGF) (AA 971-1023) protein
EGF 宿主: 人 宿主: 大肠杆菌（E. Coli） Recombinant > 95 % as determined by Tris-Bis PAGE,> 95 % as determined by HPLC
Epidermal Growth Factor (EGF) (Active) proteinEGF 宿主: 人 宿主: 大肠杆菌（E. Coli） > 95 % as determined by (a) Analysis by SEC-HPLC (b) Analysis by reducing and non-reducing SDS-PAGE Silver Stained gel Active
Epidermal Growth Factor (EGF) (AA 971-1023) protein (Fc Tag)EGF 宿主: 人 宿主: HEK-293 Cells Recombinant > 95 % as determined by Tris-Bis PAGE,> 95 % as determined by HPLC
Epidermal Growth Factor (EGF) (AA 977-1029) protein (Fc Tag)EGF 宿主: 小鼠 宿主: HEK-293 Cells Recombinant > 95 % as determined by Tris-Bis PAGE,> 95 % as determined by HPLC
Epidermal Growth Factor (EGF) (AA 971-1023) (Active) protein (Fc Tag)EGF 宿主: 人 宿主: HEK-293 Cells Recombinant >95 % as determined by SDS-PAGE. Active
Epidermal Growth Factor (EGF) (Active) proteinEGF 宿主: 小鼠 宿主: 大肠杆菌（E. Coli） Recombinant >95 % as determined by SDS-PAGE Func Active
Epidermal Growth Factor (EGF) (AA 974-1026) protein (His tag)EGF 宿主: 大鼠 宿主: 大肠杆菌（E. Coli） Recombinant Greater than 95 % as determined by SDS-PAGE WB, ELISA
Epidermal Growth Factor (EGF) (AA 926-1025) protein (GST tag)EGF 宿主: 人 宿主: 小麦胚 Recombinant WB, ELISA, AA, AP
Epidermal Growth Factor (EGF) (AA 977-1029) (Active) protein (His tag)EGF 宿主: 小鼠 宿主: 大肠杆菌（E. Coli） Recombinant > 95% by SDS-PAGE SDS, AcA Active
Epidermal Growth Factor (EGF) proteinEGF 宿主: 小鼠 宿主: 大肠杆菌（E. Coli） Recombinant 97,00% SDS
-
-
限制
- 仅限研究用
-
-
-
状态
- Lyophilized
-
溶解方式
- Please refer to the printed manual for detailed information.
-
缓冲液
- Lyophilized from sterile PBS, pH 7.4.
-
储存条件
- 4 °C,-20 °C,-80 °C
-
储存方法
- Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80°C. Reconstituted protein solution can be stored at 4-8°C for 2-7 days. Aliquots of reconstituted samples are stable at < -20°C for 3 months.
-
-
- EGF (Epidermal Growth Factor (EGF))
-
别名
- EGF
-
背景
- EGF is the founding member of the EGF-family of proteins. Members of this protein family have highly similar structural and functional characteristics. EGF contains 9 EGF-like domains and 9 LDL-receptor class B repeats. Human EGF is a 645-Da protein with 53 amino acid residues and three intramolecular disulfide bonds. As a low-molecular-weight polypeptide, EGF was first purified from the mouse submandibular gland, but since then it was found in many human tissues including submandibular gland, parotid gland. It can also be found in human platelets, macrophages, urine, saliva, milk, and plasma. EGF is a growth factor that stimulates the growth of various epidermal and epithelial tissues in vivo and in vitro and of some fibroblasts in cell culture. It results in cellular proliferation, differentiation, and survival. Salivary EGF, which seems also regulated by dietary inorganic iodine, also plays an important physiological role in the maintenance of oro-esophageal and gastric tissue integrity. EGF acts by binding with high affinity to epidermal growth factor receptor on the cell surface and stimulating the intrinsic protein-tyrosine kinase activity of the receptor. The tyrosine kinase activity, in turn, initiates a signal transduction cascade that results in a variety of biochemical changes within the cell - a rise in intracellular calcium levels, increased glycolysis and protein synthesis, and increases in the expression of certain genes including the gene for EGFR - that ultimately lead to DNA synthesis and cell proliferation.
-
分子量
- 6.3 kDa
-
NCBI登录号
- NP_036974
-
途径
- NF-kappaB Signaling, RTK signaling, Fc-epsilon Receptor Signaling Pathway, EGFR Signaling Pathway, Neurotrophin Signaling Pathway, Regulation of Carbohydrate Metabolic Process, Hepatitis C, Protein targeting to Nucleus, Interaction of EGFR with phospholipase C-gamma, Thromboxane A2 Receptor Signaling, EGFR Downregulation
抗原
-
