EPH Receptor B4 Protein (EPHB4)
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北京 101111
Quick Overview for EPH Receptor B4 Protein (EPHB4) (ABIN7195558)
抗原
See all EPH Receptor B4 (EPHB4) 蛋白蛋白类型
生物活性
宿主
资源
应用范围
纯度
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原理
- Recombinant Human EphB4/HTK Protein
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序列
- Met 1-Ala 539
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产品特性
- The mature form of human EphB4 (NP_004435.3) extracellular domain (Met 1-Ala 539) with quinary-aa peptide (DDDDK) at the C-terminus was expressed and purified.
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过滤
- 0.2 μm filtered
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内毒素水平
- < 1.0 EU per μg of the protein as determined by the LAL method.
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Biological Activity Comment
- Immobilized human EphB4 at 2 μg/ml (100 μl/well) can bind human EphrinB2 with a linear range of 1-125 ng/ml.
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EPH Receptor B4 (EPHB4) (AA 1-987) protein (GST tag)
EPHB4 宿主: 人 宿主: 小麦胚 Recombinant ELISA, WB, AA, AP
EPH Receptor B4 (EPHB4) (AA 16-539) protein (His tag)EPHB4 宿主: 小鼠 宿主: Baculovirus infected Insect Cells Recombinant > 85 % SDS
EPH Receptor B4 (EPHB4) (AA 563-987) (Active) protein (His-GST)EPHB4 宿主: 人 宿主: Baculovirus infected Insect Cells Recombinant > 90 % as determined by reducing SDS-PAGE. Active
EPH Receptor B4 (EPHB4) (Active) protein (His tag)EPHB4 宿主: 小鼠 宿主: HEK-293 Cells Recombinant > 98 % as determined by reducing SDS-PAGE. ELISA Active
EPH Receptor B4 (EPHB4) (Active) protein (Fc Tag)EPHB4 宿主: 小鼠 宿主: HEK-293 Cells Recombinant > 97 % as determined by reducing SDS-PAGE. ELISA Active
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应用备注
- Optimal working dilution should be determined by the investigator.
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限制
- 仅限研究用
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状态
- Lyophilized
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缓冲液
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Lyophilized from sterile 100 mM NaCl, 50 mM Tris, pH 7.5
Normally 5 % - 8 % trehalose, mannitol and 0.01 % Tween 80 are added as protectants before lyophilization. -
储存条件
- 4 °C,-20 °C,-80 °C
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储存方法
- Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80°C. Reconstituted protein solution can be stored at 4-8°C for 2-7 days. Aliquots of reconstituted samples are stable at < -20°C for 3 months.
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有效期
- 12 months
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- EPH Receptor B4 (EPHB4)
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别名
- EphB4/HTK
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背景
- HTK,MYK1,TYRO11,Ephrin type-B receptor 4 is a protein that in humans is encoded by the EPHB4 gene. It is a single-pass type I membrane protein belonging to the ephrin receptor subfamily of protein kinase superfamily. Members of the ephrin and Eph family are local mediators of cell function through largely contact-dependent processes in development and in maturity. Furthermore, EphB4 protein and the corresponding ligand Ephrin-B2 contribute to tumor growth in various human tumors. EphB4 protein has tumor suppressor activities and that regulation of cell proliferation, extracellular matrix remodeling, and invasive potential are important mechanisms of tumor suppression. Therefore, Ephrin-B2/EphB4 may be recognized as a novel prognostic indicator for cancers.
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分子量
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Calculated MW: 57.7 kDa
Observed MW: 65-70 kDa
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基因ID
- 2050
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NCBI登录号
- NP_004435
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UniProt
- P54760
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途径
- RTK signaling
抗原
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