EDF1 Protein (His tag)
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- 抗原 See all EDF1 蛋白
- EDF1 (Endothelial Differentiation Related Factor 1 (EDF1))
- 蛋白类型
- Recombinant
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宿主
- 人
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资源
- 大肠杆菌（E. Coli）
- 标记
- This EDF1 protein is labelled with His tag.
- 原理
- Recombinant Human EDF1/MBF1 Protein (His Tag)
- 序列
- Ala2-Lys148
- 产品特性
- Recombinant Human Endothelial Differentiation-Related Factor 1 is produced by our E.coli expression system and the target gene encoding Ala2-Lys148 is expressed with a 6His tag at the C-terminus.
- 纯度
- > 95 % as determined by reducing SDS-PAGE.
- 内毒素水平
- < 1.0 EU per μg as determined by the LAL method.
- Top Product
- Discover our top product EDF1 蛋白
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Endothelial Differentiation Related Factor 1 (EDF1) (AA 1-148) protein (His tag)
EDF1 宿主: 人 宿主: 大肠杆菌（E. Coli） Recombinant > 90 % by SDS - PAGE SDS
Endothelial Differentiation Related Factor 1 (EDF1) (AA 1-148) protein (GST tag)EDF1 宿主: 人 宿主: 小麦胚 Recombinant WB, ELISA, AA, AP
Endothelial Differentiation Related Factor 1 (EDF1) (AA 2-148) protein (His tag)Crystallography grade EDF1 宿主: 小鼠 宿主: 大肠杆菌（E. Coli） Recombinant >95 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot. SDS, WB, ELISA, Crys
Endothelial Differentiation Related Factor 1 (EDF1) protein (Myc-DYKDDDDK Tag)EDF1 宿主: 人 宿主: HEK-293 Cells Recombinant > 80 % as determined by SDS-PAGE and Coomassie blue staining AbP, STD
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- 限制
- 仅限研究用
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- 状态
- Lyophilized
- 溶解方式
- Please refer to the printed manual for detailed information.
- 缓冲液
- Lyophilized from a 0.2 μm filtered solution of 20 mM TrisHCl, 150 mM NaCl, pH 8.0.
- 储存条件
- 4 °C,-20 °C,-80 °C
- 储存方法
- Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80°C. Reconstituted protein solution can be stored at 4-8°C for 2-7 days. Aliquots of reconstituted samples are stable at < -20°C for 3 months.
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- 抗原
- EDF1 (Endothelial Differentiation Related Factor 1 (EDF1))
- 别名
- EDF1/MBF1 (EDF1 产品)
- 别名
- edf-1 Protein, mbf1 Protein, EDF-1 Protein, MBF1 Protein, 0610008L11Rik Protein, AA409425 Protein, CAP-19 Protein, zgc:73192 Protein, zgc:86829 Protein, endothelial differentiation related factor 1 S homeolog Protein, endothelial differentiation related factor 1 Protein, endothelial differentiation-related factor 1 Protein, Mbf1p Protein, edf1.S Protein, EDF1 Protein, Edf1 Protein, edf1 Protein, MBF1 Protein
- 背景
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Background: Endothelial Differentiation-Related Factor 1 (EDF1) is a 148 amino acid transcriptional coactivator that contains 1 HTH cro/C1-type DNA-binding domain. It has been postulated that the protein functions as a bridging molecule that interconnects regulatory proteins and the basal transcriptional machinery, thereby modulating the transcription of genes involved in endothelial differentiation. When endothelial cells are induced to differentiate in vitro, EDF1 is downregulated, leading to inhibition of cell growth and cell polarization. EDF1 binds calmodulin thorough its IQ domain and regulates nitric oxide synthase activity through calmodulin sequestration in the cytoplasm. Though ubiquitously expressed, EDF1 is most abundant in adult liver, heart, adipose tissues, intestine and pancreas. In fetal tissues, EDF1 is most abundant in kidney. There are two isoforms of EDF1 that are produced as a result of alternative splicing events.
Synonym: Endothelial Differentiation-Related Factor 1, EDF-1, Multiprotein-Bridging Factor 1, MBF1, EDF1
- 分子量
- 17.4 kDa
- UniProt
- O60869
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