ENTPD5 Protein (His tag)
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- 抗原 See all ENTPD5 蛋白
- ENTPD5 (Ectonucleoside Triphosphate diphosphohydrolase 5 (ENTPD5))
- 蛋白类型
- Recombinant
- 生物活性
- Active
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宿主
- 小鼠
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资源
- Baculovirus infected Insect Cells
- 标记
- This ENTPD5 protein is labelled with His tag.
- 原理
- Recombinant Mouse ENTPD5 Protein (His Tag)(Active)
- 序列
- Thr19-Ser427
- 产品特性
- A DNA sequence encoding the mouse ENTPD5 (Q9WUZ9) (Thr19-Ser427) was fused with a polyhistidine tag at the C-terminus.
- 纯度
- > 90 % as determined by SDS-PAGE
- 内毒素水平
- < 1.0 EU per μg of the protein as determined by the LAL method.
- Biological Activity Comment
- Measured by its ability to hydrolyze the 5'phosphate groups from the substrate guanosine 5'diphosphate (GDP).The specific activity is > 15000 pmols/min/μg.
- Top Product
- Discover our top product ENTPD5 蛋白
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Ectonucleoside Triphosphate diphosphohydrolase 5 (ENTPD5) (AA 1-407) protein (GST tag)
ENTPD5 宿主: 人 宿主: 小麦胚 Recombinant ELISA, WB, AA, AP
Ectonucleoside Triphosphate diphosphohydrolase 5 (ENTPD5) (AA 319-400) protein (GST tag)ENTPD5 宿主: 人 宿主: 小麦胚 Recombinant ELISA, WB, AA, AP
Ectonucleoside Triphosphate diphosphohydrolase 5 (ENTPD5) (AA 25-428) protein (His tag)Crystallography grade ENTPD5 宿主: 人 宿主: Insect Cells Recombinant >95 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot. ELISA, WB, Crys, SDS
Ectonucleoside Triphosphate diphosphohydrolase 5 (ENTPD5) (AA 25-427) protein (His tag)Crystallography grade ENTPD5 宿主: 小鼠 宿主: Insect Cells Recombinant >95 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot. ELISA, WB, Crys, SDS
Ectonucleoside Triphosphate diphosphohydrolase 5 (ENTPD5) protein (Myc-DYKDDDDK Tag)ENTPD5 宿主: 人 宿主: HEK-293 Cells Recombinant > 80 % as determined by SDS-PAGE and Coomassie blue staining AbP, STD
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- 限制
- 仅限研究用
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- 状态
- Lyophilized
- 溶解方式
- Please refer to the printed manual for detailed information.
- 缓冲液
- Lyophilized from sterile 20 mM Tris, 500 mM NaCl, 3 mM DTT, 10 % glycerol, pH 7.4
- 储存条件
- 4 °C,-20 °C,-80 °C
- 储存方法
- Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80°C. Reconstituted protein solution can be stored at 4-8°C for 2-7 days. Aliquots of reconstituted samples are stable at < -20°C for 3 months.
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- 抗原
- ENTPD5 (Ectonucleoside Triphosphate diphosphohydrolase 5 (ENTPD5))
- 别名
- ENTPD5 (ENTPD5 产品)
- 别名
- ENTPD5 Protein, CD39L4 Protein, NTPDase-5 Protein, PCPH Protein, AI196558 Protein, AI987697 Protein, Cd39l4 Protein, ER-UDPase Protein, NTPDase5 Protein, Pcph Protein, mNTPase Protein, cph Protein, ectonucleoside triphosphate diphosphohydrolase 5 Protein, ectonucleoside triphosphate diphosphohydrolase 5 L homeolog Protein, ENTPD5 Protein, entpd5 Protein, entpd5.L Protein, Entpd5 Protein
- 背景
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Background: Ectonucleoside triphosphate diphosphohydrolase 5 (ENTPD5), also known as CD39 antigen-like 4, ER-UDPase, Guanosine-diphosphatase ENTPD5, Nucleoside diphosphatase Uridine-diphosphatase ENTPD5. This hydrolase is expressed in response to phosphoinositide 3-kinase (PI3K) signaling. Activation of PI3K results in FOXO phosphorylation by AKT1 and loss of ENTPD5 transcriptional repression. It is Up-regulated in PTEN-deficient cells. Uridine diphosphatase (UDPase) that promotes protein N-glycosylation and ATP level regulation.ENTPD5 promotes protein N-glycosylation and folding in the endoplasmic reticulum, as well as elevated ATP consumption in the cytosol via an ATP hydrolysis cycle. Together with CMPK1 and AK1, ENTPD5 constitutes an ATP hydrolysis cycle that converts ATP to AMP and results in a compensatory increase in aerobic glycolysis. ENTPD5 also hydrolyzes GDP and IDP but not any other nucleoside di-, mono- or triphosphates, nor thiamine pyrophosphate. This enzyme Plays a key role in the AKT1-PTEN signaling pathway by promoting glycolysis in proliferating cells in response to phosphoinositide 3-kinase (PI3K) signaling.
Synonym: AI196558,AI987697,Cd39l4,ER-UDPase,mNTPase,NTPDase-5,NTPDase5,Pcph
- 分子量
- 46.6 kDa
- UniProt
- Q9WUZ9
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