ENTPD3 Protein (His tag)
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- 抗原 See all ENTPD3 蛋白
- ENTPD3 (Ectonucleoside Triphosphate diphosphohydrolase 3 (ENTPD3))
- 蛋白类型
- Recombinant
- 生物活性
- Active
- 宿主
- 人
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资源
- Baculovirus infected Insect Cells
- 标记
- This ENTPD3 protein is labelled with His tag.
- 原理
- Recombinant Human ENTPD3/NTPDase3/CD39L3 Protein (His Tag)(Active)
- 序列
- Gln 44-Pro 485
- 产品特性
- A DNA sequence encoding the human ENTPD3 (O75355) extracellular domain (Gln 44-Pro 485) was fused with a polyhistidine tag at the C-terminus and a signal peptide at the N-terminus.
- 纯度
- > 97 % as determined by reducing SDS-PAGE.
- 内毒素水平
- < 1.0 EU per μg of the protein as determined by the LAL method.
- Biological Activity Comment
- Measured by its ability to hydrolyze the 5'-phosphate group from the substrate adenosine-5'-triphosphate (ATP). The orthophosphate product is measured by a Malachite Green Phosphate Detection Kit.The specific activity is >70,000 pmol/min/μg.
- Top Product
- Discover our top product ENTPD3 蛋白
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Ectonucleoside Triphosphate diphosphohydrolase 3 (ENTPD3) (AA 1-452) protein (GST tag)
ENTPD3 宿主: 人 宿主: 小麦胚 Recombinant ELISA, WB, AA, AP
Ectonucleoside Triphosphate diphosphohydrolase 3 (ENTPD3) (AA 107-216) protein (GST tag)ENTPD3 宿主: 人 宿主: 小麦胚 Recombinant ELISA, WB, AA, AP
Ectonucleoside Triphosphate diphosphohydrolase 3 (ENTPD3) (AA 1-529) protein (Strep Tag)Crystallography grade ENTPD3 宿主: 人 宿主: Tobacco (Nicotiana tabacum) Recombinant >80 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot. SDS, ELISA, WB
Ectonucleoside Triphosphate diphosphohydrolase 3 (ENTPD3) (AA 44-485) protein (His tag)Crystallography grade ENTPD3 宿主: 人 宿主: Insect Cells Recombinant >95 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot. SDS, ELISA, WB, Crys
Ectonucleoside Triphosphate diphosphohydrolase 3 (ENTPD3) (AA 44-485) (Active) protein (His tag)ENTPD3 宿主: 人 宿主: Baculovirus infected Insect Cells Recombinant > 90 % by SDS - PAGE SDS Active
Ectonucleoside Triphosphate diphosphohydrolase 3 (ENTPD3) protein (Myc-DYKDDDDK Tag)ENTPD3 宿主: 人 宿主: HEK-293 Cells Recombinant > 80 % as determined by SDS-PAGE and Coomassie blue staining AbP, STD
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- 限制
- 仅限研究用
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- 状态
- Lyophilized
- 溶解方式
- Please refer to the printed manual for detailed information.
- 缓冲液
- Lyophilized from sterile 20 mM Tris, 500 mM NaCl, pH 7.4, 10 % glycerol
- 储存条件
- 4 °C,-20 °C,-80 °C
- 储存方法
- Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80°C. Reconstituted protein solution can be stored at 4-8°C for 2-7 days. Aliquots of reconstituted samples are stable at < -20°C for 3 months.
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- 抗原
- ENTPD3 (Ectonucleoside Triphosphate diphosphohydrolase 3 (ENTPD3))
- 别名
- ENTPD3/NTPDase3/CD39L3 (ENTPD3 产品)
- 别名
- ENTPD3 Protein, fi38a03 Protein, wu:fi38a03 Protein, zgc:162166 Protein, CD39L3 Protein, HB6 Protein, NTPDase-3 Protein, Cd39l3 Protein, NTPDase3 Protein, ectonucleoside triphosphate diphosphohydrolase 3 Protein, ectonucleoside triphosphate diphosphohydrolase 3 L homeolog Protein, ENTPD3 Protein, entpd3.L Protein, entpd3 Protein, Entpd3 Protein
- 背景
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Background: The ADAMs (a disintegrin and metalloprotease) comprise a family of multidomain proteins with metalloprotease, cell adhesion, and signaling activities. Human ADAM12, which is implicated in diseases such as cancer, is expressed in two splice forms, the transmembrane ADAM12-L and the shorter and soluble ADAM12-S. ADAM12, also known as and Meltrin alpha, is a member of the ADAM protein family, which contains one disintegrin domain, one EGF-like domain and one peptidase M12B domain. ADAM12 is synthesized as a zymogen with the prodomain keeping the metalloprotease inactive through a cysteine-switch mechanism. Maturation and activation of the protease involves the cleavage of the prodomain in the trans-Golgi or possibly at the cell surface by a furin-peptidase. It is a membrane-anchored metalloprotease, which has been implicated in activation-inactivation of growth factors that play an important role in wound healing, including heparin-binding epidermal growth factor (EGF)-like growth factor (HB-EGF) and IGF binding proteins. ADAM12 may also regulate cell-cell and cell-extracellular matrix contacts through interactions with cell surface receptors - integrins and syndecans - potentially influencing the actin cytoskeleton. Moreover, ADAM12 interacts with several cytoplasmic signaling and adaptor molecules through its intracellular domain, thereby directly transmitting signals to or from the cell interior. These ADAM12-mediated cellular effects appear to be critical events in both biological and pathological processes. In addition to protease activity, ADAM12 possesses cell binding and cell signaling properties. In many studies, ADAM12 overexpression has been correlated with disease, and ADAM12 has been shown to promote tumor growth and progression in cancer. On the other hand, protective effects of ADAM12 in disease have also been reported.
Synonym: CD39L3,HB6,NTPDase-3
- 分子量
- 51 kDa
- UniProt
- O75355
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