CDK1 Protein (GST tag)
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- 抗原 See all CDK1 蛋白
- CDK1 (Cyclin-Dependent Kinase 1 (CDK1))
- 蛋白类型
- Recombinant
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宿主
- 人
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资源
- Baculovirus infected Insect Cells
- 标记
- This CDK1 protein is labelled with GST tag.
- 原理
- Recombinant Human CDC2 Kinase/CDK1 Protein (GST Tag)
- 序列
- Met 1-Met 297
- 产品特性
- A DNA sequence encoding the human CDC2 isoform 1 (NP_001777.1) (Met 1-Met 297) was fused with the GST tag at the N-terminus.
- 纯度
- > 92 % as determined by reducing SDS-PAGE.
- 内毒素水平
- < 1.0 EU per μg as determined by the LAL method.
- Top Product
- Discover our top product CDK1 蛋白
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Cyclin-Dependent Kinase 1 (CDK1) (AA 1-297) protein (GST tag)
CDK1 宿主: 人 宿主: 小麦胚 Recombinant WB, ELISA, AA, AP
Cyclin-Dependent Kinase 1 (CDK1) (AA 1-433) (Active) protein (His-GST)CDK1 宿主: 人 宿主: Insect cells (Sf9) Recombinant SDS, Func Active
Cyclin-Dependent Kinase 1 (CDK1) (AA 1-297) (Active) protein (GST tag)CDK1 宿主: 人 宿主: Insect cells (Sf9) Recombinant SDS, Func Active
Cyclin-Dependent Kinase 1 (CDK1) (AA 179-297) protein (His-IF2DI Tag)CDK1 宿主: 人 宿主: 大肠杆菌（E. Coli） Recombinant Greater than 95 % by SDS-PAGE gel analyses WB, ELISA
Cyclin-Dependent Kinase 1 (CDK1) protein (GST tag,His tag)CDK1 宿主: 小鼠 宿主: Baculovirus infected Insect Cells Recombinant > 85 % as determined by SDS-PAGE
Cyclin-Dependent Kinase 1 (CDK1) (AA 211-297) protein (GST tag)CDK1 宿主: 人 宿主: 小麦胚 Recombinant WB, ELISA, AA, AP
Cyclin-Dependent Kinase 1 (CDK1) (AA 1-297) protein (Strep Tag)Crystallography grade CDK1 宿主: 小鼠 宿主: Tobacco (Nicotiana tabacum) Recombinant ≥ 80 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot. SDS, WB, ELISA
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- 限制
- 仅限研究用
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- 状态
- Lyophilized
- 溶解方式
- Please refer to the printed manual for detailed information.
- 缓冲液
- Lyophilized from sterile 50 mM Tris, 100 mM NaCl, 0.5 mM PMSF, 0.5 mM EDTA, 0.5 mM GSH, pH 8.0
- 储存条件
- 4 °C,-20 °C,-80 °C
- 储存方法
- Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80°C. Reconstituted protein solution can be stored at 4-8°C for 2-7 days. Aliquots of reconstituted samples are stable at < -20°C for 3 months.
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- 抗原
- CDK1 (Cyclin-Dependent Kinase 1 (CDK1))
- 别名
- CDC2 Kinase/CDK1 (CDK1 产品)
- 别名
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CDC2 Protein, CDC28A Protein, P34CDC2 Protein, Cdc2 Protein, Cdc2a Protein, p34
Protein, cdc2 Protein, wu:fc30e01 Protein, zgc:92032 Protein, PSTAIR Protein, cdc-2 Protein, cdc2-a Protein, cdc28a Protein, cdc2x1.1 Protein, p34cdc2 Protein, xcdc2 Protein, 5363 Protein, CDCDm Protein, CDK1 Protein, CDK1/CDC2 Protein, CG5363 Protein, Cdk-1 Protein, Cdk1 Protein, Dcdc2 Protein, Dm cdc2 Protein, DmCdc2 Protein, DmCdk1 Protein, Dmcdc2 Protein, Dmel\\CG5363 Protein, cdc Protein, cdc2Dm Protein, cdk1 Protein, dCdk1 Protein, group 4 Protein, l(2)31Eh Protein, CDC2A Protein, CDC2AAT Protein, CDK2 Protein, CDKA1 Protein, CDKA;1 Protein, CYCLIN-DEPENDENT KINASE A;1 Protein, cell division control 2 Protein, cdc2-b Protein, cdc2a Protein, cdc2x1.2 Protein, POL3 Protein, pold Protein, cyclin dependent kinase 1 Protein, cyclin dependent kinase like 1 Protein, Cell division control protein 2 1 Protein, cyclin-dependent kinase 1 Protein, cyclin-dependent kinase 1 S homeolog Protein, Cyclin-dependent kinase 1 Protein, cell division control 2 Protein, cell division control protein2 homolog Protein, cyclin-dependent kinase 1 L homeolog Protein, polymerase (DNA directed), delta 1, catalytic subunit L homeolog Protein, cyclin-dependent protein kinase Cdk1/Cdc2 Protein, CDK1 Protein, CDKL1 Protein, POPTR_0004s14080g Protein, Cdk1 Protein, cdk1 Protein, cdk1.S Protein, CDC2 Protein, cdc2 Protein, cdk-1 Protein, cdk1.L Protein, pold1.L Protein - 背景
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Background: CDC2, also known as CDK1, contains 1 protein kinase domain and belongs to the protein kinase superfamily, CMGC Ser/Thr protein kinase family, CDC2/CDKX subfamily. CDC2 is a catalytic subunit of the highly conserved protein kinase complex known as M-phase promoting factor (MPF), which is essential for G1/S and G2/M phase transitions of eukaryotic cell cycle. Mitotic cyclins stably associate with CDC2 and function as regulatory subunits. The kinase activity of CDK1 is controlled by cyclin accumulation and destruction through the cell cycle. The phosphorylation and dephosphorylation of CDC2 also play important regulatory roles in cell cycle control. It is required in higher cells for entry into S-phase and mitosis. CDC2 also is a cyclin-dependent kinase which displays CTD kinase activity and is required for RNA splicing. It has CTD kinase activity by hyperphosphorylating the C-terminal heptapeptide repeat domain (CTD) of the largest RNA polymerase II subunit RPB1, thereby acting as a key regulator of transcription elongation. CDK1 is required for RNA splicing, possibly by phosphorylating SRSF1/SF2. It is involved in regulation of MAP kinase activity, possibly leading to affect the response to estrogn inhibitors.
Synonym: CDC2,CDC28A,P34CDC2
- 分子量
- 60 kDa
- NCBI登录号
- NP_001777
- 途径
- Cell Division Cycle, Fc-epsilon Receptor Signaling Pathway, Neurotrophin Signaling Pathway, Activation of Innate immune Response, Mitotic G1-G1/S Phases, DNA Replication, M Phase, Toll-Like Receptors Cascades, Synthesis of DNA
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