CHEK1 Protein (AA 1-476) (His-GST)
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Quick Overview for CHEK1 Protein (AA 1-476) (His-GST) (ABIN7320012)
抗原
See all CHEK1 蛋白蛋白类型
生物活性
宿主
资源
纯度
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产品特性
- AA 1-476
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标记
- This CHEK1 protein is labelled with His-GST.
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原理
- Recombinant Mouse CHK1/CHEK1 Protein (His & GST Tag)
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序列
- Met1-Thr476
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产品特性
- A DNA sequence encoding the mouse CHEK1 (O35280-1) (Met1-Thr476) was fused with the N-terminal polyhistidine-tagged GST tag at the N-terminus.
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过滤
- 0.2 μm filtered
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内毒素水平
- < 1.0 EU per μg of the protein as determined by the LAL method.
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Biological Activity Comment
- Kinase activity untested
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Checkpoint Kinase 1 (CHEK1) protein (GST tag)
CHEK1 宿主: 人 宿主: 小麦胚 Recombinant WB, ELISA, AA, AP
Checkpoint Kinase 1 (CHEK1) (Active) protein (GST tag)CHEK1 宿主: 人 宿主: Baculovirus infected Insect Cells Recombinant > 90 % as determined by reducing SDS-PAGE. Active
Checkpoint Kinase 1 (CHEK1) (Transcript Variant 3) protein (Myc-DYKDDDDK Tag)CHEK1 宿主: 人 宿主: HEK-293 Cells Recombinant > 80 % as determined by SDS-PAGE and Coomassie blue staining AbP, STD
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限制
- 仅限研究用
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状态
- Liquid
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缓冲液
- Supplied as sterile solution of 20 mM Tris, 500 mM NaCl, pH 8.5, 3 mM DTT, 10 % glycerol
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储存液
- Dithiothreitol (DTT)
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注意事项
- This product contains Dithiothreitol (DTT): a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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储存条件
- -20 °C
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储存方法
- Store at < -20°C, stable for 6 months. Please minimize freeze-thaw cycles.
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有效期
- 6 months
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- CHEK1 (Checkpoint Kinase 1 (CHEK1))
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别名
- CHK1/CHEK1
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背景
- C85740,Chk1,rad27,CHK1 / CHEK1 contains 1 protein kinase domain and belongs to the protein kinase superfamily, CAMK Ser/Thr protein kinase family, NIM1 subfamily. It is a member of checkpoint kinases (Chks). Chks Checkpoint kinases (Chks) are serine/threonine kinases that are involved in the control of the cell cycle. There are two subtypes of chks that have so far been identified, CHK1 / CHEK1 and Chk2. They are essential components to delay cell cycle progression in normal and damaged cells and can act at all three cell cycle checkpoints. Chks are activated by phosphorylation. ATR kinase phosphorylates CHK1 / CHEK1 in response to single strand DNA breaks and ATM kinase phosphorylates Chk2 in response to double strand breaks. Chks phosphorylate Cdc25 phosphatase at Ser216, which leads to Cdc25 sequestration in the cytoplasm. Chks have a role in the physiological stress of hypoxia/reoxygenation. CHK1 / CHEK1 is required for checkpoint mediated cell cycle arrest in response to DNA damage or the presence of unreplicated DNA. CHK1 / CHEK1 may also negatively regulate cell cycle progression during unperturbed cell cycles.
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分子量
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Calculated MW: 82.2 kDa
Observed MW: 78 kDa
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UniProt
- O35280
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途径
- p53 Pathway, Apoptosis, Cell Division Cycle, DNA Damage Repair
抗原
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