cGMP-Dependent 3',5'-Cyclic Phosphodiesterase (PDE2A) (AA 215-900) protein (His tag)
-
- 抗原
- cGMP-Dependent 3',5'-Cyclic Phosphodiesterase (PDE2A)
- 蛋白类型
- Recombinant
- 产品特性
- AA 215-900
- 宿主
- 人
- 资源
- Baculovirus infected Insect Cells
- 标记
- His tag
- 原理
- Recombinant Human PDE2A/CGS-PDE Protein (aa 215-900, His Tag)
- 序列
- Glu 215-His 900
- 产品特性
- A DNA sequence encoding the amino acids (Glu 215-His 900) of human PDE2A (O00408-1) was fused with a polyhistidine tag at the C-terminus.
- 纯度
- > 90 % as determined by reducing SDS-PAGE.
- 内毒素水平
- < 1.0 EU per μg as determined by the LAL method.
-
- 限制
- 仅限研究用
-
- 状态
- Lyophilized
- 溶解方式
- Please refer to the printed manual for detailed information.
- 缓冲液
- Lyophilized from sterile 20 mM Tris, 500 mM NaCl, pH 7.4, 10 % glycerol
- 储存条件
- 4 °C,-20 °C,-80 °C
- 储存方法
- Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80°C. Reconstituted protein solution can be stored at 4-8°C for 2-7 days. Aliquots of reconstituted samples are stable at < -20°C for 3 months.
-
- 抗原
- cGMP-Dependent 3',5'-Cyclic Phosphodiesterase (PDE2A)
- 别名
- PDE2A/CGS-PDE
- 背景
-
Background: cGMP-dependent 3',5'-cyclic phosphodiesterase, also known as cyclic GMP-stimulated phosphodiesterase and PDE2A, is a peripheral membrane protein which belongs to the cyclic nucleotide phosphodiesterase family and PDE2 subfamily. Phosphodiesterases (PDEs) comprise a family of enzymes that regulate the levels of cyclic nucleotides, key second messengers that mediate a diverse array of functions. Phosphodiesterases (PDEs) modulate signaling by cyclic nucleotides in diverse processes such as cardiac contractility, platelet aggregation, lipolysis, glycogenolysis, and smooth muscle contraction. PDE2A is an evolutionarily conserved cGMP-stimulated cAMP and cGMP PDE. PDE2A contains two GAF domains. PDE2A is expressed in brain and to a lesser extent in heart, placenta, lung, skeletal muscle, kidney and pancreas. PDE2A is a cyclic nucleotide phosphodiesterase with a dual-specificity for the second messengers cAMP and cGMP, which are key regulators of many important physiological processes. PDE2A is involved in the regulation of blood pressure and fluid homeostasis by the atrial natriuretic peptide (ANP), making PDE2-type enzymes important targets for drug discovery.
Synonym: CGS-PDE;cGSPDE;PDE2A1;PED2A4
- 分子量
- 80.0 kDa
-
