CEACAM8 Protein (His tag)
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- 抗原 See all CEACAM8 蛋白
- CEACAM8 (Carcinoembryonic Antigen-Related Cell Adhesion Molecule 8 (CEACAM8))
- 蛋白类型
- Recombinant
- 生物活性
- Active
- 宿主
- 人
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资源
- HEK-293 Cells
- 标记
- This CEACAM8 protein is labelled with His tag.
- 原理
- Recombinant Human CEACAM8/CD66b Protein (His Tag)(Active)
- 序列
- Met 1-Ser319
- 产品特性
- A DNA sequence encoding the human CEACAM8 (NP_001807.2) without the pro peptide (Met 1-Ser319) was expressed, with a polyhistidine tag at the C-terminus.
- 纯度
- > 96 % as determined by reducing SDS-PAGE.
- 内毒素水平
- < 1.0 EU per μg as determined by the LAL method.
- Biological Activity Comment
- Measured by its binding ability in a functional ELISA. Immobilized human CEACAM6-his at 10 μg/mL (100 μl/well) can bind biotinylated human CEACAM8-his, The EC50 of biotinylated human CEACAM8-his is 0.17 μg/mL.
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- Discover our top product CEACAM8 蛋白
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Carcinoembryonic Antigen-Related Cell Adhesion Molecule 8 (CEACAM8) (AA 35-319) protein (His tag)
CEACAM8 宿主: 人 宿主: HEK-293 Cells Recombinant > 95 % as determined by Tris-Bis PAGE,> 95 % as determined by HPLC
Carcinoembryonic Antigen-Related Cell Adhesion Molecule 8 (CEACAM8) (AA 1-349) protein (GST tag)CEACAM8 宿主: 人 宿主: 小麦胚 Recombinant WB, ELISA, AA, AP
Carcinoembryonic Antigen-Related Cell Adhesion Molecule 8 (CEACAM8) (AA 35-319) protein (His tag,AVI tag,Biotin)CEACAM8 宿主: 人 宿主: HEK-293 Cells Recombinant >95 % as determined by SDS-PAGE.
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- 限制
- 仅限研究用
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- 状态
- Lyophilized
- 溶解方式
- Please refer to the printed manual for detailed information.
- 缓冲液
- Lyophilized from sterile PBS, pH 7.4
- 储存条件
- 4 °C,-20 °C,-80 °C
- 储存方法
- Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80°C. Reconstituted protein solution can be stored at 4-8°C for 2-7 days. Aliquots of reconstituted samples are stable at < -20°C for 3 months.
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- 抗原
- CEACAM8 (Carcinoembryonic Antigen-Related Cell Adhesion Molecule 8 (CEACAM8))
- 别名
- CEACAM8/CD66b (CEACAM8 产品)
- 别名
- CD67 Protein, CGM6 Protein, CD66b Protein, NCA-95 Protein, CEACAM8 Protein, CEACAM1 Protein, MGC142545 Protein, cd67 Protein, cgm6 Protein, cd66b Protein, nca-95 Protein, carcinoembryonic antigen related cell adhesion molecule 8 Protein, carcinoembryonic antigen related cell adhesion molecule 1 Protein, carcinoembryonic antigen-related cell adhesion molecule 8 Protein, CEACAM8 Protein, CEACAM1 Protein, LOC612469 Protein, ceacam8 Protein
- 背景
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Background: CEACAM8, also known as CD66b or NCA-95, is a single chain, GPI-anchored, highly glycosylated protein belonging to the carcinoembryonic antigen family. There are four members in this family: CD66a, CD66b, CD66c, and CD66d. Members of CEACAM family are widely expressed especially on human neutrophils, and, depending on the tissue, capable of regulating diverse functions including tumor promotion, tumor suppression, angiogenesis, and neutrophil activation. Abnormal overexpression and downregulation of some CEACAMs have been described in tumor cells. Monoclonal antibodies grouped in the CD66 cluster recognize CEACAM members. Ectopic CD66 expression is commonly detected in B-cell lineage acute lymphoblastic leukemia (ALL). CEACAM8(CD66b) is also an activation marker for human granulocytes. However, its biological functions are largely unknown in eosinophils. It has been reported that CD66b is highly expressed on the surface of human peripheral blood eosinophils isolated from healthy individuals. Engagement of CD66b by mAb or a natural ligand, galectin-3, activated a Src kinase family molecule, hemopoietic cell kinase (Hck), and induced cellular adhesion, superoxide production, and degranulation of eosinophils. CD66b molecules were localized in lipid rafts, and disruption of lipid rafts or removal of the GPI anchor inhibited the adhesion and activation of eosinophils. Importantly, CD66b was constitutively and physically associated with a beta2 integrin, CD11b, and cross-linking of CD66b induced a striking clustering of CD11b molecules. Thus, CD66b molecules are involved in regulating adhesion and activation of eosinophils, possibly through their localization in lipid rafts and interaction with other cell surface molecules, such as CD11b. Binding of exogenous or endogenous carbohydrate ligands(s) to CD66b may be important in the release of proinflammatory mediators by human eosinophils.
Synonym: Carcinoembryonic Antigen-Related Cell Adhesion Molecule 8, CD67 Antigen, Carcinoembryonic Antigen CGM6, Non-Specific Cross-Reacting Antigen NCA-95, CD66b, CEACAM8, CGM6
- 分子量
- 32.8 kDa
- NCBI登录号
- NP_001807
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