CPB1 Protein (His tag)
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- 抗原 See all CPB1 蛋白
- CPB1 (Carboxypeptidase B1 (Tissue) (CPB1))
- 蛋白类型
- Recombinant
- 生物活性
- Active
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宿主
- 人
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资源
- HEK-293 Cells
- 标记
- This CPB1 protein is labelled with His tag.
- 原理
- Recombinant Human Carboxypeptidase B1/CPB1 Protein (His Tag)(Active)
- 序列
- Met 1-Tyr 417
- 产品特性
- A DNA sequence encoding the human CPB1 (NP_001862.2) (Met 1-Tyr 417) was expressed with a C-terminal polyhistidine tag.
- 纯度
- > 98 % as determined by reducing SDS-PAGE.
- 内毒素水平
- < 1.0 EU per μg as determined by the LAL method.
- Biological Activity Comment
- Measured by its ability to cleave a colorimetric peptide substrate, Hippuryl-Arg, as measured using the wavelength at 254 nm.The specific activity is >10000 pmoles/min/μg.
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- Discover our top product CPB1 蛋白
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Carboxypeptidase B1 (Tissue) (CPB1) (AA 109-415) protein (His tag)
CPB1 宿主: 大鼠 宿主: 大肠杆菌（E. Coli） Recombinant Greater than 95 % as determined by SDS-PAGE WB, ELISA
Carboxypeptidase B1 (Tissue) (CPB1) (AA 14-417) protein (GST tag)CPB1 宿主: 人 宿主: 小麦胚 Recombinant WB, ELISA, AA, AP
Carboxypeptidase B1 (Tissue) (CPB1) (Active) protein (His tag)CPB1 宿主: 小鼠 宿主: HEK-293 Cells Recombinant > 94 % as determined by SDS-PAGE Active
Carboxypeptidase B1 (Tissue) (CPB1) protein (GST tag)CPB1 宿主: 人 宿主: HEK-293 Cells Recombinant > 95 % as determined by SDS-PAGE and Coomassie blue staining AbP, STD
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- 限制
- 仅限研究用
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- 状态
- Lyophilized
- 溶解方式
- Please refer to the printed manual for detailed information.
- 缓冲液
- Lyophilized from sterile 25 mM MES, 0.1 M NaCl, pH 6.5
- 储存条件
- 4 °C,-20 °C,-80 °C
- 储存方法
- Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80°C. Reconstituted protein solution can be stored at 4-8°C for 2-7 days. Aliquots of reconstituted samples are stable at < -20°C for 3 months.
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- 抗原
- CPB1 (Carboxypeptidase B1 (Tissue) (CPB1))
- 别名
- Carboxypeptidase B1/CPB1 (CPB1 产品)
- 别名
- wu:fb60d07 Protein, wu:fb69b08 Protein, CPB1 Protein, pCPB Protein, CPB Protein, PASP Protein, PCPB Protein, Cpb Protein, 0910001A18Rik Protein, 1810063F02Rik Protein, 2210008M23Rik Protein, AI504870 Protein, carboxypeptidase B1 (tissue) Protein, carboxypeptidase B1 Protein, carboxypeptidase B1 S homeolog Protein, carboxypeptidase B Protein, cpb1 Protein, CPB1 Protein, cpb1.S Protein, CpipJ_CPIJ010799 Protein, CpipJ_CPIJ010801 Protein, VDBG_02663 Protein, LOC100562609 Protein, Cpb1 Protein
- 背景
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Background: Carboxypeptidase B1, also well known as pancreatic procarboxypeptidase B (PCPB), is a highly pancreas -specific protein (PASP), and has been identified previously as a serum marker for acute pancreatitis and pancreatic graft rejection. As the prototype for those human exopeptidases that cleave off basic C-terminal residues, CPB1 specifically cleaves the C-terminal Arg and Lys residues with a preference for Arg. The B1 and B2 forms of procarboxypeptidase B differ from each other mainly in isoelectric point.The deduced amino acid sequence of PCPB predicts a 416-amino acid preproenzyme consisting of a 15-aa signal peptide, a 95-aa activation peptide and a 307-aa mature chain. The secreted PCPB zymogen is converted to enzymatically active CPB1 by limited proteolysis by trypsin.
Synonym: Carboxypeptidase B, Pancreas-Specific Protein, PASP, CPB1, CPB, PCPB
- 分子量
- 47 kDa
- NCBI登录号
- NP_001862
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