CA5B Protein (His tag)
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- 抗原 See all CA5B 蛋白
- CA5B (Carbonic Anhydrase VB, Mitochondrial (CA5B))
- 蛋白类型
- Recombinant
- 生物活性
- Active
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宿主
- 人
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资源
- 大肠杆菌（E. Coli）
- 标记
- This CA5B protein is labelled with His tag.
- 原理
- Recombinant Human Carbonic Anhydrase 5B/CA5B Protein (His Tag)(Active)
- 序列
- Cys 34-Pro 317
- 产品特性
- A DNA sequence encoding the mature form of human CA5B (Q9Y2D0) (Cys 34-Pro 317) was fused with a polyhistidine tag at the C-terminus and an initial Met at the N-terminus.
- 纯度
- > 97 % as determined by reducing SDS-PAGE.
- Biological Activity Comment
- Measured by its esterase activity. The specific activity is >150 pmoles/min/μg.
- Top Product
- Discover our top product CA5B 蛋白
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Carbonic Anhydrase VB, Mitochondrial (CA5B) (AA 1-317) protein (GST tag)
CA5B 宿主: 人 宿主: 小麦胚 Recombinant WB, ELISA, AA, AP
Carbonic Anhydrase VB, Mitochondrial (CA5B) (AA 2-90) protein (GST tag)CA5B 宿主: 人 宿主: 小麦胚 Recombinant WB, ELISA, AA, AP
Carbonic Anhydrase VB, Mitochondrial (CA5B) (AA 34-317) protein (His tag)Crystallography grade CA5B 宿主: 人 宿主: Insect Cells Recombinant >95 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot. WB, SDS, ELISA, Crys
Carbonic Anhydrase VB, Mitochondrial (CA5B) (AA 34-317) protein (His tag)Crystallography grade CA5B 宿主: 小鼠 宿主: Insect Cells Recombinant >95 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot. WB, SDS, ELISA, Crys
Carbonic Anhydrase VB, Mitochondrial (CA5B) proteinCA5B 宿主: 人 宿主: 大肠杆菌（E. Coli） Recombinant > 95 % as determined by SDS-PAGE and Coomassie blue staining AbP, STD
Carbonic Anhydrase VB, Mitochondrial (CA5B) protein (Myc-DYKDDDDK Tag)CA5B 宿主: 人 宿主: HEK-293 Cells Recombinant > 80 % as determined by SDS-PAGE and Coomassie blue staining AbP, STD
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- 限制
- 仅限研究用
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- 状态
- Lyophilized
- 溶解方式
- Please refer to the printed manual for detailed information.
- 缓冲液
- Lyophilized from sterile 20 mM Tris, 50 mM NaCl, 0.05 % Brij-35, pH 8.0
- 储存条件
- 4 °C,-20 °C,-80 °C
- 储存方法
- Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80°C. Reconstituted protein solution can be stored at 4-8°C for 2-7 days. Aliquots of reconstituted samples are stable at < -20°C for 3 months.
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- 抗原
- CA5B (Carbonic Anhydrase VB, Mitochondrial (CA5B))
- 别名
- Carbonic Anhydrase 5B/CA5B (CA5B 产品)
- 别名
- CA-VB Protein, 7330410H16Rik Protein, CAVB Protein, Ca5b Protein, CarVb Protein, D730005F19Rik Protein, zgc:171653 Protein, CA5B Protein, ca5b Protein, carbonic anhydrase 5B Protein, carbonic anhydrase 5b, mitochondrial Protein, carbonic anhydrase Va Protein, carbonic anhydrase 5A Protein, carbonic anhydrase 5B, mitochondrial Protein, carbonic anhydrase VB, mitochondrial L homeolog Protein, CA5B Protein, Car5b Protein, Ca5b Protein, ca5a Protein, CA5A Protein, LOC100051095 Protein, ca5b.L Protein
- 背景
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Background: Carbonic anhydrase 5B; also known as carbonate dehydratase VB; carbonic anhydrase VB; CA-VB and CA5B; is a member of the alpha-carbonic anhydrase family. The strongest expression of CA5B / CA-VB is in heart; pancreas; kidney; placenta; lung; and skeletal muscle. It is not expressed in liver. Carbonic anhydrases (CAs) are a large family of zinc metalloenzymes first discovered in 1933 that catalyze the reversible hydration of carbon dioxide. CAs participate in a variety of biological processes; including respiration; calcification; acid-base balance; bone resorption; and the formation of aqueous humor; cerebrospinal fluid; saliva; and gastric acid. CAs show extensive diversity in tissue distribution and in their subcellular localization. CA5B / CA-VB is localized in the mitochondria and shows the highest sequence similarity to the other mitochondrial CA5A / CA-VA. CA5B / CA-VB has a wider tissue distribution than CA5A / CA-VA; which is restricted to the liver. The differences in tissue distribution suggest that the two mitochondrial carbonic anhydrases evolved to assume different physiologic roles. CA5A / CA-VA is activated by histamine; L-adrenaline; L- and D-histidine; and L- and D-phenylalanine. It is inhibited by coumarins; sulfonamide derivatives such as acetazolamide and Foscarnet (phosphonoformate trisodium salt). CA5B / CA-VB is inhibited by coumarins; sulfonamide derivatives such as acetazolamide (AZA); saccharin and Foscarnet (phosphonoformate trisodium salt).
Synonym: Carbonic Anhydrase 5B Mitochondrial; Carbonate Dehydratase VB; Carbonic Anhydrase VB; CA-VB; CA5B
- 分子量
- 34 kDa
- UniProt
- Q9Y2D0
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