CA3 Protein (His tag)
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- 抗原 See all CA3 蛋白
- CA3 (Carbonic Anhydrase III (CA3))
- 蛋白类型
- Recombinant
- 生物活性
- Active
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宿主
- 人
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资源
- 大肠杆菌（E. Coli）
- 标记
- This CA3 protein is labelled with His tag.
- 原理
- Recombinant Human Carbonic Anhydrase 3/CA3 Protein (His Tag)(Active)
- 序列
- Met 1-Lys 260
- 产品特性
- A DNA sequence encoding the human CA3 (NP_005172.1) (Met 1-Lys 260) was expressed, with a polyhistide tag at the C-terminus.
- 纯度
- > 95 % as determined by reducing SDS-PAGE.
- Biological Activity Comment
- Measured by its esterase activity.The specific activity is >5 pmoles/min/μg.
- Top Product
- Discover our top product CA3 蛋白
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Carbonic Anhydrase III (CA3) (AA 1-260) protein (GST tag)
CA3 宿主: 人 宿主: 小麦胚 Recombinant WB, ELISA, AA, AP
Carbonic Anhydrase III (CA3) (AA 2-260) protein (His tag)Crystallography grade CA3 宿主: 人 宿主: Insect Cells Recombinant >95 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot. WB, SDS, ELISA, Crys
Carbonic Anhydrase III (CA3) (AA 2-260) protein (His tag)Crystallography grade CA3 宿主: 小鼠 宿主: Insect Cells Recombinant >95 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot. WB, SDS, ELISA, Crys
Carbonic Anhydrase III (CA3) protein (Myc-DYKDDDDK Tag)CA3 宿主: 人 宿主: HEK-293 Cells Recombinant > 80 % as determined by SDS-PAGE and Coomassie blue staining AbP, STD
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- 限制
- 仅限研究用
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- 状态
- Lyophilized
- 溶解方式
- Please refer to the printed manual for detailed information.
- 缓冲液
- Lyophilized from sterile 50 mM Tris, 500 mM NaCl, 10 % glycerol, pH 8.0
- 储存条件
- 4 °C,-20 °C,-80 °C
- 储存方法
- Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80°C. Reconstituted protein solution can be stored at 4-8°C for 2-7 days. Aliquots of reconstituted samples are stable at < -20°C for 3 months.
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- 抗原
- CA3 (Carbonic Anhydrase III (CA3))
- 别名
- Carbonic Anhydrase 3/CA3 (CA3 产品)
- 别名
- CAIII Protein, Car3 Protein, BB219044 Protein, Ca3 Protein, Car-3 Protein, carbonic anhydrase 3 Protein, CA3 Protein, CAH3 Protein, Car3 Protein, Ca3 Protein
- 背景
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Background: Carbonic anhydrases (CAs) are a large family of zinc metalloenzymes first discovered in 1933 that catalyze the reversible hydration of carbon dioxide. CAs participate in a variety of biological processes, including respiration, calcification, acid-base balance, bone resorption, and the formation of aqueous humor, cerebrospinal fluid, saliva, and gastric acid. Carbonic anhydrases (CAs) form a family of enzymes that catalyze the rapid conversion of carbon dioxide and water to bicarbonate and protons, a reaction that occurs rather slowly in the absence of a catalyst. The active site of most carbonic anhydrases contains a zinc ion, they are therefore classified as metalloenzymes. Several forms of carbonic anhydrase occur in nature. The primary function of the enzyme in animals is to interconvert carbon dioxide and bicarbonate to maintain acid-base balance in blood and other tissues, and to help transport carbon dioxide out of tissues. Plants contain a different form called β-carbonic anhydrase, which, from an evolutionary standpoint, is a distinct enzyme, but participates in the same reaction and also uses a zinc ion in its active site. Carbonic anhydrase 3, also known as Carbonate dehydratase III, CA-III and CA3, is a cytoplasm protein which belongs to the alpha-carbonic anhydrase family. CA3 is activated by proton donors such as imidazole and the dipeptide histidylhistidine. It is inhibited by coumarins and sulfonamide derivatives such as acetazolamide. At 6 weeks gestation, transcripts accumulate at low levels in the somites and at high levels throughout the notochord. As gestation continues, CA3 becomes abundant in all developing muscle masses and continues at high to moderate levels in the notochord.
Synonym: Carbonic Anhydrase 3; Carbonate Dehydratase III; Carbonic Anhydrase III; CA-III; CA3;Car3
- 分子量
- 30.4 kDa
- NCBI登录号
- NP_005172
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