BLMH Protein (His tag)
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- 抗原 See all BLMH 蛋白
- BLMH (Bleomycin Hydrolase (BLMH))
- 蛋白类型
- Recombinant
- 生物活性
- Active
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宿主
- 小鼠
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资源
- 大肠杆菌（E. Coli）
- 标记
- This BLMH protein is labelled with His tag.
- 原理
- Recombinant Mouse BLMH/BLM Hydrolase Protein (His Tag)(Active)
- 序列
- Asn 2-Glu 455
- 产品特性
- A DNA sequence encoding the mouse BLMH (NP_848760.1) (Asn 2-Glu 455) was expressed, with a polyhistide tag at the N-terminus.
- 纯度
- > 95 % as determined by SDS-PAGE
- Biological Activity Comment
- Measured by its ability to hydrolyze Met-AMC. The specific activity is >500 pmoles/min/μg.
- Top Product
- Discover our top product BLMH 蛋白
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Bleomycin Hydrolase (BLMH) (AA 1-455) protein (GST tag)
BLMH 宿主: 人 宿主: 小麦胚 Recombinant WB, ELISA, AA, AP
Bleomycin Hydrolase (BLMH) (AA 356-454) protein (GST tag)BLMH 宿主: 人 宿主: 小麦胚 Recombinant WB, ELISA, AA, AP
Bleomycin Hydrolase (BLMH) (AA 1-455) protein (His tag)BLMH 宿主: 人 宿主: 大肠杆菌（E. Coli） Recombinant Greater than 95 % as determined by SDS-PAGE WB, ELISA
Bleomycin Hydrolase (BLMH) (AA 1-455) protein (Strep Tag)Crystallography grade BLMH 宿主: 人 宿主: Tobacco (Nicotiana tabacum) Recombinant >80 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot. WB, SDS, ELISA
Bleomycin Hydrolase (BLMH) (AA 1-455) protein (Strep Tag)Crystallography grade BLMH 宿主: 小鼠 宿主: Tobacco (Nicotiana tabacum) Recombinant ≥ 80 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot. WB, SDS, ELISA
Bleomycin Hydrolase (BLMH) proteinBLMH 宿主: 小鼠 宿主: 大肠杆菌（E. Coli） Recombinant > 95 % WB, SDS, ELISA, IP
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- 限制
- 仅限研究用
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- 状态
- Lyophilized
- 溶解方式
- Please refer to the printed manual for detailed information.
- 缓冲液
- Lyophilized from sterile 50 mM Tris, 0.15M NaCl, 10 % glycerol, pH 8.0
- 储存条件
- 4 °C,-20 °C,-80 °C
- 储存方法
- Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80°C. Reconstituted protein solution can be stored at 4-8°C for 2-7 days. Aliquots of reconstituted samples are stable at < -20°C for 3 months.
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- 抗原
- BLMH (Bleomycin Hydrolase (BLMH))
- 别名
- BLMH/BLM Hydrolase (BLMH 产品)
- 别名
- AI035728 Protein, Bh Protein, Bmh Protein, wu:fb13c01 Protein, zgc:66261 Protein, bh Protein, bmh Protein, BH Protein, BMH Protein, bleomycin hydrolase Protein, Bleomycin hydrolase Protein, bleomycin hydrolase S homeolog Protein, BLMH Protein, pepC Protein, LOC5569945 Protein, Apre_0140 Protein, Apar_0899 Protein, Smon_1289 Protein, Kfla_1242 Protein, Snas_3912 Protein, BLJ_1941 Protein, Olsu_1085 Protein, PGTDC60_0106 Protein, Blmh Protein, blmh Protein, blmh.S Protein
- 背景
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Background: The papain superfamily member bleomycin hydrolase (BLMH) is a cytoplasmic cysteine peptidase that is highly conserved through evolution. The only known activity of the enzyme is metabolic inactivation of the glycopeptide bleomycin (BLM), an essential component of combination chemotherapy regimens for cancer. The papain superfamily member bleomycin hydrolase (BLMH) is a neutral cysteine protease with structural similarity to a 20S proteasome. Bleomycin (BLM), a clinically used glycopeptide anticancer agent. BLMH is an essential protectant against BLM-induced death and has an important role in neonatal survival and in maintaining epidermal integrity. Sequencing revealed several putative sites phosphorylated by different types of protein kinases, but no signal sequence, transmembrane domain, N-linked glycosylation site or DNA-binding motif.
Synonym: AI035728,Bh,Bmh
- 分子量
- 53.3 kDa
- NCBI登录号
- NP_848760
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