CD57 Protein (His tag)
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- 抗原 See all CD57 (B3GAT1) 蛋白
- CD57 (B3GAT1) (beta-1,3-Glucuronyltransferase 1 (Glucuronosyltransferase P) (B3GAT1))
- 蛋白类型
- Recombinant
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宿主
- 人
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资源
- 人细胞
- 标记
- This CD57 protein is labelled with His tag.
- 原理
- Recombinant Human B3GAT1 (N-6His)
- 序列
- His25-Ile334
- 产品特性
- Recombinant Human Galactosylgalactosylxylosylprotein 3-beta-glucuronosyltransferase 1 is produced by our Mammalian expression system and the target gene encoding His25-Ile334 is expressed with a 6His tag at the N-terminus.
- 纯度
- >95 % as determined by reducing SDS-PAGE.
- 内毒素水平
- < 1.0 EU per μg as determined by the LAL method.
- Top Product
- Discover our top product B3GAT1 蛋白
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beta-1,3-Glucuronyltransferase 1 (Glucuronosyltransferase P) (B3GAT1) (AA 35-334) protein (His tag)
B3GAT1 宿主: 人 宿主: 大肠杆菌（E. Coli） Recombinant Greater than 95 % by SDS-PAGE gel analyses WB, ELISA
beta-1,3-Glucuronyltransferase 1 (Glucuronosyltransferase P) (B3GAT1) (AA 235-332) protein (GST tag)B3GAT1 宿主: 人 宿主: 小麦胚 Recombinant WB, ELISA, AA, AP
beta-1,3-Glucuronyltransferase 1 (Glucuronosyltransferase P) (B3GAT1) (AA 28-334) protein (GST tag)B3GAT1 宿主: 人 宿主: 小麦胚 Recombinant WB, ELISA, AA, AP
beta-1,3-Glucuronyltransferase 1 (Glucuronosyltransferase P) (B3GAT1) (AA 1-334) protein (Strep Tag)Crystallography grade B3GAT1 宿主: 人 宿主: Tobacco (Nicotiana tabacum) Recombinant >80 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot. WB, ELISA, SDS
beta-1,3-Glucuronyltransferase 1 (Glucuronosyltransferase P) (B3GAT1) (AA 1-334) protein (Strep Tag)Crystallography grade B3GAT1 宿主: 小鼠 宿主: Tobacco (Nicotiana tabacum) Recombinant ≥ 80 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot. WB, ELISA, SDS
beta-1,3-Glucuronyltransferase 1 (Glucuronosyltransferase P) (B3GAT1) (AA 28-334) protein (His tag)Crystallography grade B3GAT1 宿主: 人 宿主: Insect Cells Recombinant >95 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot. WB, ELISA, SDS, Crys
beta-1,3-Glucuronyltransferase 1 (Glucuronosyltransferase P) (B3GAT1) (AA 28-334) protein (His tag)Crystallography grade B3GAT1 宿主: 小鼠 宿主: Insect Cells Recombinant >95 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot. WB, ELISA, SDS, Crys
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- 限制
- 仅限研究用
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- 状态
- Lyophilized
- 溶解方式
- Please refer to the printed manual for detailed information.
- 缓冲液
- Lyophilized from a 0.2 μm filtered solution of 20 mM Tris-HCl, 150 mM NaCl, pH 8.0.
- 储存条件
- 4 °C,-20 °C,-80 °C
- 储存方法
- Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80°C. Reconstituted protein solution can be stored at 4-8°C for 2-7 days. Aliquots of reconstituted samples are stable at < -20°C for 3 months.
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- 抗原
- CD57 (B3GAT1) (beta-1,3-Glucuronyltransferase 1 (Glucuronosyltransferase P) (B3GAT1))
- 别名
- B3GAT1 (B3GAT1 产品)
- 别名
- CD57 Protein, GLCATP Protein, GLCUATP Protein, HNK1 Protein, LEU7 Protein, NK-1 Protein, NK1 Protein, 0710007K08Rik Protein, AI846286 Protein, GlcAT-P Protein, Glcatp Protein, Hnk1 Protein, Hnk-1 Protein, b3gat1 Protein, si:dkey-22o20.1 Protein, cd57 Protein, glcatp Protein, glcuatp Protein, hnk-1 Protein, hnk1 Protein, leu7 Protein, nk-1 Protein, nk1 Protein, beta-1,3-glucuronyltransferase 1 Protein, beta-1,3-glucuronyltransferase 1 (glucuronosyltransferase P) Protein, beta-1,3-glucuronyltransferase 1 (glucuronosyltransferase P) a Protein, beta-1,3-glucuronyltransferase 1 L homeolog Protein, B3GAT1 Protein, B3gat1 Protein, b3gat1a Protein, b3gat1.L Protein
- 背景
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Background: B3GAT1 is the key enzyme during the biosynthesis of the carbohydrate epitope HNK-1, which is present on a number of cell adhesion molecules important in neurodevelopment. It adds a glucuronic residue to the terminal lactosamine residue (Gal beta 14GlcNAc) of a glycoprotein or glycolipid, which can be further sulfated to become the HNK1 epitope, a unique trisaccharide structure, HSO3-3GlcA beta 1-3Gal beta 1-4GlcNAc. The enzyme activity was found to be enhanced in the presence of sphingomyelin and phosphatidylinositol. The HNK1 carbohydrate epitope is characteristically expressed on a series of cell adhesion molecules in addition to some glycolipids in the extracellular matrix and on the cell surface in the nervous system, where it is involved in cell-cell and cell-substratum interaction and recognition during the development of the nervous system. Like most known glycosyltransferases, B3GAT1 is a type II Golgi-resident transmembrane protein with a short N-terminal cytoplasmic domain and a single pass transmembrane domain followed by an enzymatic domain in the lumen of Golgi apparatus. The enzyme activity was assayed using a phosphatase-coupled method.
Synonym: B3GAT1, Galactosylgalactosylxylosylprotein 3-beta-glucuronosyltransferase 1, beta-1,3-glucuronyltransferase 1 (glucuronosyltransferase P), CD57, GlcAT-P, HNK1, NK1, NK-1
- 分子量
- 36.2 kDa
- UniProt
- Q9P2W7
- 途径
- Glycosaminoglycan Metabolic Process
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