Arylsulfatase A Protein (ARSA) (His tag)
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- 抗原 See all Arylsulfatase A (ARSA) 蛋白
- Arylsulfatase A (ARSA)
- 蛋白类型
- Recombinant
- 生物活性
- Active
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宿主
- 小鼠
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资源
- HEK-293 Cells
- 标记
- This Arylsulfatase A protein is labelled with His tag.
- 原理
- Recombinant Mouse Arylsulfatase A/ARSA Protein (His Tag)(Active)
- 序列
- Met 1-Ser 506
- 产品特性
- A DNA sequence encoding the extracellular domain (Met 1-Ser 506) of mouse ARSA (NP_033843.2) precursor was expressed with a C-terminal polyhistidine tag.
- 纯度
- > 97 % as determined by SDS-PAGE
- 内毒素水平
- < 1.0 EU per μg of the protein as determined by the LAL method.
- Biological Activity Comment
- Measured by its ability to cleave p-Nitrocatechol Sulfate (PNCS). The specific activity is >100 pmoles/min/μg.
- Top Product
- Discover our top product ARSA 蛋白
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Arylsulfatase A (ARSA) (AA 367-485) protein (His-IF2DI Tag)
ARSA 宿主: 人 宿主: 大肠杆菌（E. Coli） Recombinant Greater than 95 % by SDS-PAGE gel analyses WB, ELISA
Arylsulfatase A (ARSA) (AA 1-507) protein (GST tag)ARSA 宿主: 人 宿主: 小麦胚 Recombinant WB, ELISA, AA, AP
Arylsulfatase A (ARSA) (AA 398-507) protein (GST tag)ARSA 宿主: 人 宿主: 小麦胚 Recombinant WB, ELISA, AA, AP
Arylsulfatase A (ARSA) (AA 448-507) protein (His tag)Crystallography grade ARSA 宿主: 人 宿主: Insect Cells Recombinant >95 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot. SDS, WB, ELISA, Crys
Arylsulfatase A (ARSA) (AA 19-444) protein (His tag)Crystallography grade ARSA 宿主: 人 宿主: Insect Cells Recombinant >95 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot. SDS, WB, ELISA, Crys
Arylsulfatase A (ARSA) (AA 19-507) protein (His tag)Crystallography grade ARSA 宿主: 人 宿主: Insect Cells Recombinant >95 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot. SDS, WB, ELISA, Crys
Arylsulfatase A (ARSA) (AA 18-506) protein (His tag)Crystallography grade ARSA 宿主: 小鼠 宿主: Insect Cells Recombinant >95 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot. SDS, WB, ELISA, Crys
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- 限制
- 仅限研究用
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- 状态
- Lyophilized
- 溶解方式
- Please refer to the printed manual for detailed information.
- 缓冲液
- Lyophilized from sterile 25 mM Tris, 0.15 mM NaCl, pH 7.4
- 储存条件
- 4 °C,-20 °C,-80 °C
- 储存方法
- Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80°C. Reconstituted protein solution can be stored at 4-8°C for 2-7 days. Aliquots of reconstituted samples are stable at < -20°C for 3 months.
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- 抗原
- Arylsulfatase A (ARSA)
- 别名
- Arylsulfatase A/ARSA (ARSA 产品)
- 别名
- ARSA Protein, zgc:101575 Protein, arsa Protein, AS-A Protein, ASA Protein, AW212749 Protein, As-2 Protein, As2 Protein, TISP73 Protein, MLD Protein, mld Protein, arylsulfatase A Protein, arylsulfatase Protein, arylsulfatase A, gene 1 S homeolog Protein, ARSA Protein, arsa Protein, arsA Protein, RB6599 Protein, Arsa Protein, arsa.1.S Protein
- 背景
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Background: Arylsulfatase A (ARSA) is synthesized as a 52KDa lysosomal enzyme. It is a member of the sulfatase family that is required for the lysosomal degradation of cerebroside-3-sulfate, a sphingolipid sulfate ester and a major constituent of the myelin sheet. Arylsulfatase A is activated by a required co- or posttranslational modification with the oxidation of cysteine to formylglycine. Metachromatic leukodystrophy (MLD) is a lysosomal storage disease in the central and peripheral nervous systems with severe and progressive neurological symptoms caused by the deficiency of Arylsulfatase A. Deficiency of this enzyme is also found in apparently healthy individuals, a condition for which the term pseudodeficiency is introduced. ARSA forms dimers after receiving three N-linked oligosaccharides in the endoplasmic reticulum, and then the dimers are transported to the Golgi where they receive mannose 6-phosphate recognition markers. And thus, ARSA is transported and delivered to dense lysosomes in a mannose 6-phosphate receptor-dependent manner. It has been shown that within the lysosomes, the ARSA dimers can oligomerize to an octamer in a pH -dependent manner. The ARSA deficiency leads to metachromatic leucodystrophy (MLD), a lysosomal storage disorder associated with severe and progressive demyelination in he central and peripheral nervous system. Additionally, the serum level of arylsulfatase A might be helpful in diagnosis of lung and central nervous system cancer.
Synonym: As-2,AS-A,As2,ASA,AW212749,TISP73
- 分子量
- 53.5 kDa
- NCBI登录号
- NP_033843
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