ATF2 Protein (His-GST)
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北京 101111
Quick Overview for ATF2 Protein (His-GST) (ABIN7194113)
抗原
See all ATF2 蛋白蛋白类型
宿主
资源
纯度
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标记
- This ATF2 protein is labelled with His-GST.
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原理
- Recombinant Human ATF2 Protein (His & GST Tag)
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序列
- Met 1-Ser 505
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产品特性
- A DNA sequence encoding full length of human ATF2 (P15336-1) (Met 1-Ser 505) was fused with the N-terminal polyhistidine-tagged GST tag at the N-terminus.
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过滤
- 0.2 μm filtered
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内毒素水平
- < 1.0 EU per μg of the protein as determined by the LAL method.
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Biological Activity Comment
- Not validated for activity
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Activating Transcription Factor 2 (ATF2) (AA 1-209) protein (GST tag)
ATF2 宿主: 人 宿主: 小麦胚 Recombinant ELISA, WB, AA, AP
Activating Transcription Factor 2 (ATF2) protein (His tag)ATF2 宿主: 人 宿主: 大肠杆菌（E. Coli） Recombinant > 80 % as determined by SDS-PAGE and Coomassie blue staining AbP, STD
Activating Transcription Factor 2 (ATF2) (AA 1-505), (full length) protein (His-SUMO Tag)ATF2 宿主: 人 宿主: 大肠杆菌（E. Coli） Recombinant Greater than 90 % as determined by SDS-PAGE. SDS
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限制
- 仅限研究用
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状态
- Lyophilized
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缓冲液
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Lyophilized from sterile 20 mM Tris, 500 mM NaCl, pH 8.0, 10 % glycerol
Normally 5 % - 8 % trehalose, mannitol and 0.01 % Tween 80 are added as protectants before lyophilization. -
储存条件
- 4 °C,-20 °C,-80 °C
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储存方法
- Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80°C. Reconstituted protein solution can be stored at 4-8°C for 2-7 days. Aliquots of reconstituted samples are stable at < -20°C for 3 months.
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有效期
- 12 months
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- ATF2 (Activating Transcription Factor 2 (ATF2))
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别名
- ATF2
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背景
- CRE-BP1;CREB-2;CREB2;HB16;TREB7,Activating transcription factor 2, also known as ATF2, is a member of the leucine zipper family of DNA-binding proteins that binds to the cAMP response element. Its activity is enhanced after phosphorylation by stress-activated protein kinases such as c-Jun N-terminal kinase and p38. ATF2 has been found to be a target of the JNK signal transduction pathway and mediate adenovirus E1A-inducible transcriptional activation. ATF2 is also been reported playing roles in TGF-β signaling pathway. It has been shown that the transcription factor ATF2 is bound by a hetero-oligomer of Smad3 and Smad4 upon TGF-β stimulation. Studies indicate that ATF-2 plays a central role in TGF-β signaling by acting as a common nuclear target of both Smad and TAK1 pathways.
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分子量
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Calculated MW: 82.4 kDa
Observed MW: 85 kDa
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基因ID
- 1386
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UniProt
- P15336
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途径
- MAPK Pathway, RTK signaling, Thyroid Hormone Synthesis, Activation of Innate immune Response, Chromatin Binding, Myometrial Relaxation and Contraction, Synaptic Membrane, Tube Formation, Toll-Like Receptors Cascades
抗原
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