AIM2 Protein (GST tag)
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- 抗原 See all AIM2 蛋白
- AIM2 (Absent in Melanoma 2 (AIM2))
- 蛋白类型
- Recombinant
- 生物活性
- Active
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宿主
- 人
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资源
- Baculovirus infected Insect Cells
- 标记
- This AIM2 protein is labelled with GST tag.
- 原理
- Recombinant Human AIM2 Protein (GST Tag)(Active)
- 序列
- Met 1-Thr 343
- 产品特性
- A DNA sequence encoding the human AIM2 (NP_004824.1) (Met 1-Thr 343) was fused with the GST tag at the N-terminus.
- 纯度
- > 95 % as determined by reducing SDS-PAGE.
- 内毒素水平
- < 1.0 EU per μg as determined by the LAL method.
- Biological Activity Comment
- Measured by its ability to inhibit the proliferation of MCF7 human breast adenocarcinoma cells. The ED50 for this effect is typically 10-40ug/ml.
- Top Product
- Discover our top product AIM2 蛋白
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Absent in Melanoma 2 (AIM2) (AA 1-356) protein (GST tag)
AIM2 宿主: 人 宿主: 小麦胚 Recombinant WB, ELISA, AA, AP
Absent in Melanoma 2 (AIM2) (AA 1-354) protein (Strep Tag)Crystallography grade AIM2 宿主: 小鼠 宿主: Tobacco (Nicotiana tabacum) Recombinant ≥ 80 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot. WB, SDS, ELISA
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- 限制
- 仅限研究用
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- 状态
- Lyophilized
- 溶解方式
- Please refer to the printed manual for detailed information.
- 缓冲液
- Lyophilized from sterile 50 mM Tris, 1M NaCl, 0.5 mM PMSF, 5 mM GSH, pH 8.0
- 储存条件
- 4 °C,-20 °C,-80 °C
- 储存方法
- Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80°C. Reconstituted protein solution can be stored at 4-8°C for 2-7 days. Aliquots of reconstituted samples are stable at < -20°C for 3 months.
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- 抗原
- AIM2 (Absent in Melanoma 2 (AIM2))
- 别名
- AIM2 (AIM2 产品)
- 别名
- AIM2 Protein, PYHIN4 Protein, Gm1313 Protein, Ifi210 Protein, absent in melanoma 2 Protein, AIM2 Protein, Aim2 Protein
- 背景
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Background: AIM2, Absent In Melanoma 2 is a member of the interferon-inducible HIN-200 protein family that contains an amino-terminal pyrin domain and a carboxy-terminal oligonucleotide/oligosaccharide-binding domain, senses cytoplasmic DNA by means of its oligonucleotide/oligosaccharide-binding domain and interacts with ASC (apoptosis-associated speck-like protein containing a CARD) through its pyrin domain to activate caspase-1. In response to foreign cytoplasmic DNA, AIM2 forms an inflammasome, resulting in caspase activation in inflammatory cells. It had been pointed to a role of AIM2 function in both inflammation and cancer. AIM-2 antigen is expressed in a wide variety of tumor types, including neuroectodermal tumors, as well as breast, ovarian and colon carcinomas. AIM-2 could be used as a tumor antigen target for monitoring vaccine trials or to develop antigen specific active immunotherapy for glioma patients.
Synonym: PYHIN4
- 分子量
- 65.2 kDa
- NCBI登录号
- NP_004824
- 途径
- Activation of Innate immune Response, Positive Regulation of Endopeptidase Activity, Inflammasome
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