PAPSS1 Protein (His tag)
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- 抗原 See all PAPSS1 蛋白
- PAPSS1 (3'-phosphoadenosine 5'-phosphosulfate Synthase 1 (PAPSS1))
- 蛋白类型
- Recombinant
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宿主
- 人
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资源
- 人细胞
- 标记
- This PAPSS1 protein is labelled with His tag.
- 原理
- Recombinant Human PAPS Synthase 1/PAPSS1 Protein (His Tag)
- 序列
- Met 1-Ala624
- 产品特性
- Recombinant Human Bifunctional 3'-phosphoadenosine 5'-phosphosulfate synthase 1 is produced by our Mammalian expression system and the target gene encoding Met1-Ala624(O43252) is expressed with a 6His tag at the C-terminus.
- 纯度
- > 95 % as determined by reducing SDS-PAGE.
- 内毒素水平
- < 1.0 EU per μg as determined by the LAL method.
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- Discover our top product PAPSS1 蛋白
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3'-phosphoadenosine 5'-phosphosulfate Synthase 1 (PAPSS1) (AA 24-624) protein (His tag)
PAPSS1 宿主: 人 宿主: 大肠杆菌（E. Coli） Recombinant > 85 % by SDS - PAGE SDS
3'-phosphoadenosine 5'-phosphosulfate Synthase 1 (PAPSS1) (AA 1-603) protein (GST tag)PAPSS1 宿主: 人 宿主: 小麦胚 Recombinant ELISA, WB, AP, AA
3'-phosphoadenosine 5'-phosphosulfate Synthase 1 (PAPSS1) (AA 144-252) protein (GST tag)PAPSS1 宿主: 人 宿主: 小麦胚 Recombinant ELISA, WB, AP, AA
3'-phosphoadenosine 5'-phosphosulfate Synthase 1 (PAPSS1) (AA 1-624) protein (Strep Tag)Crystallography grade PAPSS1 宿主: 人 宿主: Tobacco (Nicotiana tabacum) Recombinant >80 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot. SDS, ELISA, WB
3'-phosphoadenosine 5'-phosphosulfate Synthase 1 (PAPSS1) (AA 1-624) protein (Strep Tag)Crystallography grade PAPSS1 宿主: 小鼠 宿主: Tobacco (Nicotiana tabacum) Recombinant ≥ 80 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot. SDS, ELISA, WB
3'-phosphoadenosine 5'-phosphosulfate Synthase 1 (PAPSS1) protein (Myc-DYKDDDDK Tag)PAPSS1 宿主: 人 宿主: HEK-293 Cells Recombinant > 80 % as determined by SDS-PAGE and Coomassie blue staining AbP, STD
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- 限制
- 仅限研究用
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- 状态
- Frozen, Liquid
- 缓冲液
- Supplied as a 0.2 μm filtered solution of 20 mM Tris,100 mM NaCl,20 % glycerol, pH 8.0.
- 储存条件
- -20 °C
- 储存方法
- Store at < -20°C, stable for 6 months. Please minimize freeze-thaw cycles.
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- 抗原
- PAPSS1 (3'-phosphoadenosine 5'-phosphosulfate Synthase 1 (PAPSS1))
- 别名
- PAPS Synthase 1/PAPSS1 (PAPSS1 产品)
- 别名
- ATPSK1 Protein, PAPSS Protein, SK1 Protein, AI325286 Protein, Asapk Protein, papss1 Protein, MGC82588 Protein, atpsk1 Protein, papss Protein, sk1 Protein, PAPSS1 Protein, wu:fc43e12 Protein, zgc:194984 Protein, zgc:194985 Protein, CG8363 Protein, DmPAPSS Protein, Dmel\\CG8363 Protein, PAPS Protein, Paps Protein, l(3)76BDn Protein, paps Protein, 3'-phosphoadenosine 5'-phosphosulfate synthase 1 Protein, bifunctional 3'-phosphoadenosine 5'-phosphosulfate synthase 1 Protein, bifunctional 3'-phosphoadenosine 5'-phosphosulfate synthase 2 Protein, PAPS synthetase Protein, PAPSS1 Protein, Papss1 Protein, papss1 Protein, papss1.L Protein, LOC100543301 Protein, LOC100637112 Protein, Papss Protein
- 背景
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Background: PAPSS1 is a bifunctional enzyme with both ATP sulfurylase and APS kinase activity. In the N-terminal section, it belongs to the APS kinase family, while the C-terminal section belongs to the sulfate adenylyltransferase family. PAPSS1 can be inhibited by chlorate, and is expressed in many tissues, such as high endothelial venules (HEV) cells and in cartilage. PAPSS1 mediates two steps in the sulfate activation pathway. The first step is the transfer of a sulfate group to ATP to yield adenosine 5'-phosphosulfate (APS), and the second step is the transfer of a phosphate group from ATP to APS yielding 3'-phosphoadenylylsulfate. In mammals, PAPS is the sole source of sulfate, APS appears to be only an intermediate in the sulfate-activation pathway. PAPSS1 also involved in the biosynthesis of sulfated L-selectin ligands in endothelial cells.
Synonym: Bifunctional 3'-phosphoadenosine 5'-phosphosulfate synthase 1,PAPS synthase 1,PAPSS 1,Sulfurylase kinase 1,SK 1,ATPSK1, PAPSS
- 分子量
- 71.9 kDa
- UniProt
- O43252
- 途径
- Glycosaminoglycan Metabolic Process, Ribonucleoside Biosynthetic Process
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