CCL1 Protein (His-Avi Tag,Biotin)
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- 抗原 See all CCL1 蛋白
- CCL1 (Chemokine (C-C Motif) Ligand 1 (CCL1))
- 蛋白类型
- Recombinant
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宿主
- 人
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资源
- HEK-293 Cells
- 标记
- This CCL1 protein is labelled with His-Avi Tag,Biotin.
- 原理
- Biotinylated Human CCL1 / I-309 / C-C motif chemokine 1 Protein, His,Avitag™ (MALS verified)
- 序列
- Lys 24 - Lys 96
- 产品特性
- Biotinylated Human CCL1, His,Avitag is expressed from human 293 cells (HEK293). It contains AA Lys 24 - Lys 96 (Accession # P22362-1).
- 纯度
- >90 % as determined by SDS-PAGE.
- 内毒素水平
- Less than 1.0 EU per μg by the LAL method.
- 质量等级
- MALS verified
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Chemokine (C-C Motif) Ligand 1 (CCL1) (AA 24-96) protein (His tag)
CCL1 宿主: 人 宿主: 大肠杆菌（E. Coli） Recombinant Greater than 95 % as determined by SDS-PAGE WB, ELISA
Chemokine (C-C Motif) Ligand 1 (CCL1) (AA 1-96) protein (GST tag)CCL1 宿主: 人 宿主: 小麦胚 Recombinant WB, ELISA, AA, AP
Chemokine (C-C Motif) Ligand 1 (CCL1) (AA 24-96) protein (GST tag)CCL1 宿主: 人 宿主: 小麦胚 Recombinant WB, ELISA, AA, AP
Chemokine (C-C Motif) Ligand 1 (CCL1) (AA 24-96) protein (His tag)Crystallography grade CCL1 宿主: 人 宿主: Insect Cells Recombinant >95 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot. WB, SDS, ELISA, Crys
Chemokine (C-C Motif) Ligand 1 (CCL1) (AA 24-92) protein (His tag)Crystallography grade CCL1 宿主: 小鼠 宿主: Insect Cells Recombinant >95 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot. WB, SDS, ELISA, Crys
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- 应用备注
- This protein carries a polyhistidine tag at the C-terminus, followed by an Avi tag (Avitag™). The protein has a calculated MW of 12.1 kDa. The protein migrates as 15 kDa and 17-19 kDa under reducing (R) condition due to glycosylation.
- 说明
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Ready-to-use Avitag™ biotinylated protein:
The product is exclusively produced using the Avitag™ technology. Briefly, a unique 15 amino acid peptide, the Avi tag, is introduced into the recombinant protein during expression vector construction. The single lysine residue in the Avi tag is enzymatically biotinylated by the E. Coli biotin ligase BirA.
This single-point enzymatic labeling technique brings many advantages for commonly used binding assays. The biotinylation happens on the lysine residue of Avi tag, and therefore does NOT interfere with the target protein's natural binding activities. In addition, when immobilized on an avidin-coated surface, the protein orientation is uniform because the position of the Avi tag in the protein is precisely controlled. - 限制
- 仅限研究用
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- 状态
- Lyophilized
- 缓冲液
- PBS, pH 7.4
- 储存条件
- -20 °C
- 储存方法
- For long term storage, the product should be stored at lyophilized state at -20°C or lower.
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- 抗原
- CCL1 (Chemokine (C-C Motif) Ligand 1 (CCL1))
- 别名
- CCL1 / I-309 / C-C motif chemokine 1 (CCL1 产品)
- 别名
- I-309 Protein, P500 Protein, SCYA1 Protein, SISe Protein, TCA3 Protein, BF534335 Protein, Scya1 Protein, Tca-3 Protein, CCLi6 Protein, chemokine Protein, C-C motif chemokine ligand 1 Protein, chemokine (C-C motif) ligand 1 Protein, CCL1 Protein, Ccl1 Protein
- 背景
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Synonyms: CCL1/I-309/C-C motif chemokine 1,
Description: Chemokines form a superfamily of secreted proteins involved in immunoregulatory and inflammatory processes. The superfamily is divided into four subfamilies based on the arrangement of the N-terminal cysteine residues of the mature peptide. CCL1, a member of the CC subfamily, is secreted by activated T cells and displays chemotactic activity for monocytes but not for neutrophils. It binds to the chemokine (C-C motif) receptor 8. - 分子量
- 12.1 kDa
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