Annexin A11 Protein (ANXA11) (full length) (rho-1D4 tag)
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- 抗原 See all Annexin A11 (ANXA11) 蛋白
- Annexin A11 (ANXA11)
- 蛋白类型
- Recombinant
- 产品特性
- full length
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宿主
- CHO cells
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资源
- Insect Cells
- 标记
- This Annexin A11 protein is labelled with rho-1D4 tag.
- 应用范围
- SDS-PAGE (SDS), Western Blotting (WB), ELISA, Crystallization (Crys), Functional Studies (Func)
- 序列
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MAQVLRGTVT AFPGFDSRAD AETLRKAMKG LGTDEESILT LLTSRSNAQR QEIAEEFKTL FGKDLLDDLK SELTGKFEKL IVALMKPSRL YDAYELKHAL KGAGTNEKVL TEIIASRTPE ELRVIKQVYE EEYGSSLEDD VVGDTSGYYQ RMLVVLLQAN RDPDTAIDDA QVELDAQALF QAGELKWGTD EEKFITIFGT RSVSHLRRVF DKYMTISGFQ IEETIDRETS GNLEQLLLAV VKSIRSIPAY LAETLYYAMK GAGTDDHTLI RVVVSRSEID LYNIRKEFRK NFATSLYSMI KGDTSGDYKK TLLLLCGGED D
Sequence without tag. The location of the tag depends on protein. You may also submit your preference when ordering. - 产品特性
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- Made in Germany - from design to production - by highly experienced protein experts.
- CHO Annexin Protein (raised in Insect Cells) purified by multi-step, protein-specific process to ensure crystallization grade.
- State-of-the-art algorithm used for plasmid design (Gene synthesis).
This protein is a custom-made protein and will be made for the first time for your order. This protein will be produced on the basis of on a Custom Service Project. We will make sure that every step in the production is successful from the design of the expression plasmid to the expression and purification of the final protein. Our experts in the lab will ensure that you receive a correctly folded protein.
The concentration of our recombinant proteins is measured using the absorbance at 280nm. The protein's absorbance will be measured in several dilutions and is measured against its specific reference buffer. The concentration of the protein is calculated using its specific absorption coefficient. We use the Expasy's protparam tool to determine the absorption coefficient of each protein.
- 纯化方法
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Three step purification of proteins expressed in baculovirus infected SF9 insect cells:
- Membrane proteins are fractioned by ultracentrifugation and subsequently solubilized with different detergents (detergent screen). Samples are analyzed by Western blot.
- The best performing detergent is used for solubilization and the proteins are purified via their rho1D4 tag via two rho1D4 antibody columns: one DTT resistant, the other one not. Eluate fractions are analyzed by Western blot.
- Protein containing fractions of the best purification are subjected to second purification step through size exclusion chromatograph. Eluate fractions are analyzed by SDS-PAGE and Western blot.
- 纯度
- >95 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot.
- 过滤
- 0.22 μm filtered
- 内毒素水平
- Endotoxins have not been removed. Please contact us if you require an endotoxin-free version of this product.
- 质量等级
- Crystallography grade
- Biological Activity Comment
- Protein has not been tested for activity yet.
- Top Product
- Discover our top product ANXA11 蛋白
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Annexin A11 (ANXA11) (AA 1-505) protein (GST tag)
ANXA11 宿主: 人 宿主: 小麦胚 Recombinant WB, ELISA, AA, AP
Annexin A11 (ANXA11) (AA 406-505) protein (GST tag)ANXA11 宿主: 人 宿主: 小麦胚 Recombinant WB, ELISA, AA, AP
Annexin A11 (ANXA11) (AA 1-505) protein (Strep Tag)Crystallography grade ANXA11 宿主: 人 宿主: Tobacco (Nicotiana tabacum) Recombinant >80 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot. SDS, WB, ELISA
Annexin A11 (ANXA11) (AA 1-503) protein (Strep Tag)Crystallography grade ANXA11 宿主: 小鼠 宿主: Tobacco (Nicotiana tabacum) Recombinant ≥ 80 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot. SDS, WB, ELISA
Annexin A11 (ANXA11) (Transcript Variant B) protein (Myc-DYKDDDDK Tag)ANXA11 宿主: 人 宿主: HEK-293 Cells Recombinant > 80 % as determined by SDS-PAGE and Coomassie blue staining AbP, STD
Annexin A11 (ANXA11) (Transcript Variant A) protein (Myc-DYKDDDDK Tag)ANXA11 宿主: 人 宿主: HEK-293 Cells Recombinant > 80 % as determined by SDS-PAGE and Coomassie blue staining AbP, STD
Annexin A11 (ANXA11) (Transcript Variant C) protein (Myc-DYKDDDDK Tag)ANXA11 宿主: 人 宿主: HEK-293 Cells Recombinant > 80 % as determined by SDS-PAGE and Coomassie blue staining AbP, STD
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- 应用备注
- Optimal working dilution should be determined by the investigator.
- 限制
- 仅限研究用
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- 状态
- Liquid
- 缓冲液
- 150 mM NaCL, 20 mM NaH2PO4 pH 7.4, 10 % glycerol. Note: Isoelectric point of protein taken into account regarding pH .
- 注意事项
- Avoid repeated freeze-thaw cycles.
- 储存条件
- -80 °C
- 储存方法
- Store at -80°C.
- 有效期
- Unlimited (if stored properly)
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- 抗原
- Annexin A11 (ANXA11)
- 别名
- Annexin (ANXA11 产品)
- 别名
- anxa11 Protein, MGC89007 Protein, ANXA11 Protein, anx11 Protein, LOC100226075 Protein, cap50 Protein, ANX11 Protein, CAP50 Protein, A830099O17Rik Protein, Anx11 Protein, annexin A11 Protein, Annexin A11 Protein, annexin A11 S homeolog Protein, anxa11 Protein, ANXA11 Protein, anx11 Protein, anxa11.S Protein, Anxa11 Protein
- 背景
- This protein is an anticoagulant protein that acts as an indirect inhibitor of the thromboplastin-specific complex, which is involved in the blood coagulation cascade. {ECO:0000256|ARBA:ARBA00002758}.
- UniProt
- G3I5A4
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