Lipoprotein Lipase Protein (LPL) (full length) (rho-1D4 tag)
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- 抗原 See all Lipoprotein Lipase (LPL) 蛋白
- Lipoprotein Lipase (LPL)
- 蛋白类型
- Recombinant
- 产品特性
- full length
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宿主
- CHO cells
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资源
- Insect Cells
- 标记
- This Lipoprotein Lipase protein is labelled with rho-1D4 tag.
- 应用范围
- Western Blotting (WB), SDS-PAGE (SDS), ELISA, Crystallization (Crys), Functional Studies (Func)
- 序列
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MAAADGGRDF TDIESKFALR TPDDTAEDNC HLIPGIAESV SNCHFNHSSK TFVVIHGWTV TGMYESWVPK LVAALYKREP DSNVIVVDWL YRAQQHYPVS AGYTKLVGND VARFINWMEE EFNYPLDNVH LLGYSLGAHA AGVAGSLTNK KVNRITGLDP AGPNFEYAEA PSRLSPDDAD FVDVLHTFTR GSPGRSIGIQ KPVGHVDIYP NGGTFQPGCN IGEAIRVIAE RGLGDVDQLV KCSHERSIHL FIDSLLNEEN PSKAYRCNSK EAFEKGLCLS CRKNRCNNVG YEINKVRAKR SSKMYLKTRS QMPYKVFHYQ VKIHFSGTES DKQLNQAFEI SLYGTVAESE NIPFTLPEVS TNKTYSFLIY TEVDIGELLM MKLKWKSDSY FSWSDWWSSP GFVIEKIRVK AGETQKKVIF CAREKVSHLQ KGKDSAVFVK CHDKSLKKSG
Sequence without tag. The location of the tag depends on protein. You may also submit your preference when ordering. - 产品特性
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- Made in Germany - from design to production - by highly experienced protein experts.
- CHO Lipoprotein lipase (LPL) Protein (raised in Insect Cells) purified by multi-step, protein-specific process to ensure crystallization grade.
- State-of-the-art algorithm used for plasmid design (Gene synthesis).
This protein is a custom-made protein and will be made for the first time for your order. This protein will be produced on the basis of on a Custom Service Project. We will make sure that every step in the production is successful from the design of the expression plasmid to the expression and purification of the final protein. Our experts in the lab will ensure that you receive a correctly folded protein.
The concentration of our recombinant proteins is measured using the absorbance at 280nm. The protein's absorbance will be measured in several dilutions and is measured against its specific reference buffer. The concentration of the protein is calculated using its specific absorption coefficient. We use the Expasy's protparam tool to determine the absorption coefficient of each protein.
- 纯化方法
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Three step purification of proteins expressed in baculovirus infected SF9 insect cells:
- Membrane proteins are fractioned by ultracentrifugation and subsequently solubilized with different detergents (detergent screen). Samples are analyzed by Western blot.
- The best performing detergent is used for solubilization and the proteins are purified via their rho1D4 tag via two rho1D4 antibody columns: one DTT resistant, the other one not. Eluate fractions are analyzed by Western blot.
- Protein containing fractions of the best purification are subjected to second purification step through size exclusion chromatograph. Eluate fractions are analyzed by SDS-PAGE and Western blot.
- 纯度
- >95 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot.
- 过滤
- 0.22 μm filtered
- 内毒素水平
- Endotoxins have not been removed. Please contact us if you require an endotoxin-free version of this product.
- 质量等级
- Crystallography grade
- Biological Activity Comment
- Protein has not been tested for activity yet.
- Top Product
- Discover our top product LPL 蛋白
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Lipoprotein Lipase (LPL) (AA 311-475) protein (His-IF2DI Tag)
LPL 宿主: 人 宿主: 大肠杆菌（E. Coli） Recombinant Greater than 95 % by SDS-PAGE gel analyses WB, ELISA
Lipoprotein Lipase (LPL) (AA 1-475) protein (GST tag)LPL 宿主: 人 宿主: 小麦胚 Recombinant WB, ELISA, AP, AA
Lipoprotein Lipase (LPL) (AA 28-474) protein (His tag)Crystallography grade LPL 宿主: 小鼠 宿主: Insect Cells Recombinant >95 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot. WB, SDS, ELISA, Crys
Lipoprotein Lipase (LPL) protein (Myc-DYKDDDDK Tag)LPL 宿主: 人 宿主: HEK-293 Cells Recombinant > 80 % as determined by SDS-PAGE and Coomassie blue staining AbP, STD
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- 应用备注
- Optimal working dilution should be determined by the investigator.
- 限制
- 仅限研究用
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- 状态
- Liquid
- 缓冲液
- 150 mM NaCL, 20 mM NaH2PO4 pH 7.4, 10 % glycerol. Note: Isoelectric point of protein taken into account regarding pH .
- 注意事项
- Avoid repeated freeze-thaw cycles.
- 储存条件
- -80 °C
- 储存方法
- Store at -80°C.
- 有效期
- Unlimited (if stored properly)
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- 抗原
- Lipoprotein Lipase (LPL)
- Abstract
- LPL 产品
- 别名
- HDLCQ11 Protein, LIPD Protein, fb62e04 Protein, fc49b03 Protein, wu:fb62e04 Protein, wu:fc49b03 Protein, LPL Protein, lipd Protein, hdlcq11 Protein, lpl Protein, LOC100223817 Protein, lipoprotein lipase Protein, LPL Protein, Lpl Protein, lpl Protein
- 背景
- Key enzyme in triglyceride metabolism. Catalyzes the hydrolysis of triglycerides from circulating chylomicrons and very low density lipoproteins (VLDL), and thereby plays an important role in lipid clearance from the blood stream, lipid utilization and storage. Mediates margination of triglyceride-rich lipoprotein particles in capillaries. Recruited to its site of action on the luminal surface of vascular endothelium by binding to GPIHBP1 and cell surface heparan sulfate proteoglycans. {ECO:0000256|RuleBase:RU362020}.
- UniProt
- G3H6V7
- 途径
- Lipid Metabolism
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