PCOLCE Protein (full length) (rho-1D4 tag)
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- 抗原 See all PCOLCE 蛋白
- PCOLCE (Procollagen C-Endopeptidase Enhancer (PCOLCE))
- 蛋白类型
- Recombinant
- 产品特性
- full length
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宿主
- CHO cells
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资源
- Insect Cells
- 标记
- This PCOLCE protein is labelled with rho-1D4 tag.
- 应用范围
- SDS-PAGE (SDS), Western Blotting (WB), ELISA, Crystallization (Crys), Functional Studies (Func)
- 序列
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MLPAVLTSLL GPFLVAWVLP LARGQTPNYT RPVFLCGGDV TGESGYVASE GFPNLYPPNK KCIWTITVPE GQTVSLSFRV FDMELHPSCR YDALEVFAGS GTSGQRLGRF CGTFRPAPVV APGNQVTLRM TTDEGTGGRG FLLWYSGRAT SGTGPPPGAR RKWVMDPRVE WAAWGYWDEH QFCGGRMEKA QGTLTTPNWP ESDYPPGISC SWHIIAPSDQ VLTLLSRLPT SQCLYQVIML TFGKFDVEPD TYCRYDSVSV FNGAVSDDSK RLGKFCGDKA PSPISSEGNE LLVQFVSDLS VTADGFSASY KTLPRDAVEK ELAPSPGEDV QLGPQSRSDP KTGTGPKVKP PSKPKFQPAE KPEVSPDTQE TPVAPDPPSA TCPKQYKRLG TLQSNFCASS LVVTGTVKTM VRGPGEGLTV TISLLGVYKS GGLDLPSPPT DTSLKLYVPC RQMPPMKKGA SYLLMGQVEE NRGPILPPES FLVPYKPNQD QILNNLRKRK CPSQPRPAA
Sequence without tag. The location of the tag depends on protein. You may also submit your preference when ordering. - 产品特性
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- Made in Germany - from design to production - by highly experienced protein experts.
- CHO Procollagen C-endopeptidase enhancer 1 Protein (raised in Insect Cells) purified by multi-step, protein-specific process to ensure crystallization grade.
- State-of-the-art algorithm used for plasmid design (Gene synthesis).
This protein is a custom-made protein and will be made for the first time for your order. This protein will be produced on the basis of on a Custom Service Project. We will make sure that every step in the production is successful from the design of the expression plasmid to the expression and purification of the final protein. Our experts in the lab will ensure that you receive a correctly folded protein.
The concentration of our recombinant proteins is measured using the absorbance at 280nm. The protein's absorbance will be measured in several dilutions and is measured against its specific reference buffer. The concentration of the protein is calculated using its specific absorption coefficient. We use the Expasy's protparam tool to determine the absorption coefficient of each protein.
- 纯化方法
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Three step purification of proteins expressed in baculovirus infected SF9 insect cells:
- Membrane proteins are fractioned by ultracentrifugation and subsequently solubilized with different detergents (detergent screen). Samples are analyzed by Western blot.
- The best performing detergent is used for solubilization and the proteins are purified via their rho1D4 tag via two rho1D4 antibody columns: one DTT resistant, the other one not. Eluate fractions are analyzed by Western blot.
- Protein containing fractions of the best purification are subjected to second purification step through size exclusion chromatograph. Eluate fractions are analyzed by SDS-PAGE and Western blot.
- 纯度
- >95 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot.
- 过滤
- 0.22 μm filtered
- 内毒素水平
- Endotoxins have not been removed. Please contact us if you require an endotoxin-free version of this product.
- 质量等级
- Crystallography grade
- Biological Activity Comment
- Protein has not been tested for activity yet.
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- Discover our top product PCOLCE 蛋白
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Procollagen C-Endopeptidase Enhancer (PCOLCE) (AA 26-449) protein (His tag)Crystallography grade PCOLCE 宿主: 人 宿主: Insect Cells Recombinant >95 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot. SDS, WB, ELISA, Crys
Procollagen C-Endopeptidase Enhancer (PCOLCE) (AA 25-468) protein (His tag)Crystallography grade PCOLCE 宿主: 小鼠 宿主: Insect Cells Recombinant >95 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot. SDS, WB, ELISA, Crys
Procollagen C-Endopeptidase Enhancer (PCOLCE) protein (Myc-DYKDDDDK Tag)PCOLCE 宿主: 人 宿主: HEK-293 Cells Recombinant > 80 % as determined by SDS-PAGE and Coomassie blue staining AbP, STD
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- 应用备注
- Optimal working dilution should be determined by the investigator.
- 限制
- 仅限研究用
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- 状态
- Liquid
- 缓冲液
- 150 mM NaCL, 20 mM NaH2PO4 pH 7.4, 10 % glycerol. Note: Isoelectric point of protein taken into account regarding pH .
- 注意事项
- Avoid repeated freeze-thaw cycles.
- 储存条件
- -80 °C
- 储存方法
- Store at -80°C.
- 有效期
- Unlimited (if stored properly)
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- 抗原
- PCOLCE (Procollagen C-Endopeptidase Enhancer (PCOLCE))
- 别名
- Procollagen C-endopeptidase enhancer 1 (PCOLCE 产品)
- 别名
- PCPE Protein, PCPE-1 Protein, PCPE1 Protein, Astt-2 Protein, Astt2 Protein, P14 Protein, procollagen C-endopeptidase enhancer Protein, procollagen C-endopeptidase enhancer L homeolog Protein, procollagen C-endopeptidase enhancer protein Protein, PCOLCE Protein, Pcolce Protein, pcolce.L Protein
- UniProt
- G3I664
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