Catalase Protein (CAT) (full length) (rho-1D4 tag)
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- 抗原 See all Catalase (CAT) 蛋白
- Catalase (CAT)
- 蛋白类型
- Recombinant
- 产品特性
- full length
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宿主
- CHO cells
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资源
- Insect Cells
- 标记
- This Catalase protein is labelled with rho-1D4 tag.
- 应用范围
- Western Blotting (WB), SDS-PAGE (SDS), ELISA, Crystallization (Crys), Functional Studies (Func)
- 序列
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MRFLTPGGDF QRAQLSCSPR SAAPKSYTMA DSRDPASDQM KHWKEQRSLQ KPDVLTTGGG NPIGDKLNIM TAGSRGPLLV QDVVFTDEMA HFDRERIPER VVHAKGAGAF GYFEVTHDIT RYCKAKVFEH IGKRTPIAVR FSTVAGESGS ADTVRDPRGF AVKFYTEDGN WDLVGNNTPI FFIRDAILFP SFIHSQKRNP QTHLKDPDMV WDFWSLRPES LHQVSFLFSD RGIPDGHRHM NGYGSHTFKL VNANGEAVYC KFHYKTDQGI KNLPVAEAAR LTQEDPDYGL RDLFNAIAQG NYPSWTFYIQ VMTFKEAETF PFNPFDLTKI WPHQDYPLIP VGKLVLNRNP VNYFAEVEQM AFDPSNMPPG IEPSPDKMLQ GRLFAYPDTH RHRLGPNYLQ IPVNCPYRAR VANYQRDGPM CMHDNQGGAP NYYPNSFSAP EQQRSALEHR SQCSTDVQRF NSANEDNVTQ VRNFYTKVLN EEERKRLCEN IAGHLKDAQL FIQKKAVKNF TDVHPDYGAR IQALLDKYNA EKPKNAIHTY TQAGSHLAAK EKANL
Sequence without tag. The location of the tag depends on protein. You may also submit your preference when ordering. - 产品特性
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- Made in Germany - from design to production - by highly experienced protein experts.
- CHO Catalase Protein (raised in Insect Cells) purified by multi-step, protein-specific process to ensure crystallization grade.
- State-of-the-art algorithm used for plasmid design (Gene synthesis).
This protein is a custom-made protein and will be made for the first time for your order. This protein will be produced on the basis of on a Custom Service Project. We will make sure that every step in the production is successful from the design of the expression plasmid to the expression and purification of the final protein. Our experts in the lab will ensure that you receive a correctly folded protein.
The concentration of our recombinant proteins is measured using the absorbance at 280nm. The protein's absorbance will be measured in several dilutions and is measured against its specific reference buffer. The concentration of the protein is calculated using its specific absorption coefficient. We use the Expasy's protparam tool to determine the absorption coefficient of each protein.
- 纯化方法
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Three step purification of proteins expressed in baculovirus infected SF9 insect cells:
- Membrane proteins are fractioned by ultracentrifugation and subsequently solubilized with different detergents (detergent screen). Samples are analyzed by Western blot.
- The best performing detergent is used for solubilization and the proteins are purified via their rho1D4 tag via two rho1D4 antibody columns: one DTT resistant, the other one not. Eluate fractions are analyzed by Western blot.
- Protein containing fractions of the best purification are subjected to second purification step through size exclusion chromatograph. Eluate fractions are analyzed by SDS-PAGE and Western blot.
- 纯度
- >95 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot.
- 过滤
- 0.22 μm filtered
- 内毒素水平
- Endotoxins have not been removed. Please contact us if you require an endotoxin-free version of this product.
- 质量等级
- Crystallography grade
- Biological Activity Comment
- Protein has not been tested for activity yet.
- Top Product
- Discover our top product CAT 蛋白
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Catalase (CAT) protein (Fc Tag,His tag)
CAT 宿主: 人 宿主: HEK-293 Cells Recombinant The purity of the protein is greater than 95 % as determined by SDS-PAGE and Coomassie blue staining. WB, ELISA
Catalase (CAT) (AA 1-527) protein (GST tag)CAT 宿主: 人 宿主: 小麦胚 Recombinant WB, ELISA, AA, AP
Catalase (CAT) (AA 1-100) protein (GST tag)CAT 宿主: 人 宿主: 小麦胚 Recombinant WB, ELISA, AA, AP
Catalase (CAT) (AA 1-527) protein (His tag)CAT 宿主: 人 宿主: 大肠杆菌（E. Coli） Recombinant Greater than 95 % as determined by SDS-PAGE WB, ELISA
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- 应用备注
- Optimal working dilution should be determined by the investigator.
- 限制
- 仅限研究用
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- 状态
- Liquid
- 缓冲液
- 150 mM NaCL, 20 mM NaH2PO4 pH 7.4, 10 % glycerol. Note: Isoelectric point of protein taken into account regarding pH .
- 注意事项
- Avoid repeated freeze-thaw cycles.
- 储存条件
- -80 °C
- 储存方法
- Store at -80°C.
- 有效期
- Unlimited (if stored properly)
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- 抗原
- Catalase (CAT)
- 别名
- Catalase (CAT 产品)
- 别名
- 2210418N07 Protein, Cas-1 Protein, Cas1 Protein, Cs-1 Protein, CS1 Protein, Cat01 Protein, Catl Protein, fb68a12 Protein, wu:fb68a12 Protein, CAT Protein, CATA Protein, CG6871 Protein, CT21282 Protein, CatA Protein, DMCATHPO Protein, DROCATHPO Protein, Dmel\\CG6871 Protein, U00145 Protein, bs36h11.y1 Protein, cat Protein, GB11648 Protein, catalase Protein, Cat Protein, BA0843 Protein, DKFZp469E0232 Protein, catalase Protein, Catalase Protein, catalase, gene 2 Protein, CAT Protein, Cat Protein, cat Protein, cat.2 Protein, LOC769804 Protein, BA_0843 Protein
- 背景
- Occurs in almost all aerobically respiring organisms and serves to protect cells from the toxic effects of hydrogen peroxide. {ECO:0000256|RuleBase:RU004142}.
- UniProt
- G3GYY6
- 途径
- Cellular Glucan Metabolic Process, Cell RedoxHomeostasis, Photoperiodism
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