PLOD1 Protein (full length) (rho-1D4 tag)
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- 抗原 See all PLOD1 蛋白
- PLOD1 (Procollagen-Lysine,2-Oxoglutarate 5-Dioxygenase 1 (PLOD1))
- 蛋白类型
- Recombinant
- 产品特性
- full length
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宿主
- CHO cells
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资源
- Insect Cells
- 标记
- This PLOD1 protein is labelled with rho-1D4 tag.
- 应用范围
- Western Blotting (WB), SDS-PAGE (SDS), ELISA, Crystallization (Crys), Functional Studies (Func)
- 序列
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MKTDKRLSDK EVPPQGSADN LLVLTVATKE TEGFRRFKRS AQFFNYKIQW VPSLDPASPS PRFGHSYDVV FASGPRELLK KFQQAKSRVV FSAEELIYPD RRLEAKYPTV SDGKRFLGSG GFIGYAPNLN KLVAEWEGQD SDSDQLFYTK IFLDPEKREQ INISLGHSCS IFQNLDGALD EVVLKFEMGH VRARNLAYDT LPVVIHGNGP TKLQLNYLGN YIPRFWTFET GCTVCDEGLR SLKGIGDEAL PTVLVGVFIE QPTPFLSLFF LRLLRLRYPQ KRMRLFIHNH EQHHKLEVEK FLAEHGTEYQ SVKLVGPEVR MANADARNMG ADLCRQDQTC TYYFSVDADV ALTEPDSLRL LIEQNKNVIA PLMTRHGRLW SNFWGALSAD GYYARSEDYV DIVQGRRVGV WNVPYISNIY LIKGSALRAE LQHVDLFHYS KLDADMSFCA NVRQQEVFMF LTNRHTFGHL LSLDNYQTTH LHNDLWEVFS NPEDWKEKYI HENYTKALEG KLVEMPCPDV YWFPIFTEAA CDELVEEMEH YGQWSLGDNK DNRIQGGYEN VPTIDIHMNQ ITFEREWHKF LVEYIAPMTE KLYPGYYTRA QFDLAFVVRY KPDEQPSLMP HHDASTFTVN IALNRVGQDY EVSQGLGAQR QVEGGARLF
Sequence without tag. The location of the tag depends on protein. You may also submit your preference when ordering. - 产品特性
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- Made in Germany - from design to production - by highly experienced protein experts.
- CHO Procollagen-lysine,2-oxoglutarate 5-dioxygenase 1 Protein (raised in Insect Cells) purified by multi-step, protein-specific process to ensure crystallization grade.
- State-of-the-art algorithm used for plasmid design (Gene synthesis).
This protein is a custom-made protein and will be made for the first time for your order. This protein will be produced on the basis of on a Custom Service Project. We will make sure that every step in the production is successful from the design of the expression plasmid to the expression and purification of the final protein. Our experts in the lab will ensure that you receive a correctly folded protein.
The concentration of our recombinant proteins is measured using the absorbance at 280nm. The protein's absorbance will be measured in several dilutions and is measured against its specific reference buffer. The concentration of the protein is calculated using its specific absorption coefficient. We use the Expasy's protparam tool to determine the absorption coefficient of each protein.
- 纯化方法
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Three step purification of proteins expressed in baculovirus infected SF9 insect cells:
- Membrane proteins are fractioned by ultracentrifugation and subsequently solubilized with different detergents (detergent screen). Samples are analyzed by Western blot.
- The best performing detergent is used for solubilization and the proteins are purified via their rho1D4 tag via two rho1D4 antibody columns: one DTT resistant, the other one not. Eluate fractions are analyzed by Western blot.
- Protein containing fractions of the best purification are subjected to second purification step through size exclusion chromatograph. Eluate fractions are analyzed by SDS-PAGE and Western blot.
- 纯度
- >95 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot.
- 过滤
- 0.22 μm filtered
- 内毒素水平
- Endotoxins have not been removed. Please contact us if you require an endotoxin-free version of this product.
- 质量等级
- Crystallography grade
- Biological Activity Comment
- Protein has not been tested for activity yet.
- Top Product
- Discover our top product PLOD1 蛋白
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Procollagen-Lysine,2-Oxoglutarate 5-Dioxygenase 1 (PLOD1) (AA 1-727) protein (GST tag)
PLOD1 宿主: 人 宿主: 小麦胚 Recombinant WB, ELISA, AP, AA
Procollagen-Lysine,2-Oxoglutarate 5-Dioxygenase 1 (PLOD1) (AA 19-114) protein (GST tag)PLOD1 宿主: 人 宿主: 小麦胚 Recombinant WB, ELISA, AP, AA
Procollagen-Lysine,2-Oxoglutarate 5-Dioxygenase 1 (PLOD1) (AA 19-727) protein (His tag)Crystallography grade PLOD1 宿主: 人 宿主: Insect Cells Recombinant >95 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot. WB, SDS, ELISA, Crys
Procollagen-Lysine,2-Oxoglutarate 5-Dioxygenase 1 (PLOD1) (AA 19-728) protein (His tag)Crystallography grade PLOD1 宿主: 小鼠 宿主: Insect Cells Recombinant >95 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot. WB, SDS, ELISA, Crys
Procollagen-Lysine,2-Oxoglutarate 5-Dioxygenase 1 (PLOD1) protein (Myc-DYKDDDDK Tag)PLOD1 宿主: 人 宿主: HEK-293 Cells Recombinant > 80 % as determined by SDS-PAGE and Coomassie blue staining AbP, STD
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- 应用备注
- Optimal working dilution should be determined by the investigator.
- 限制
- 仅限研究用
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- 状态
- Liquid
- 缓冲液
- 150 mM NaCL, 20 mM NaH2PO4 pH 7.4, 10 % glycerol. Note: Isoelectric point of protein taken into account regarding pH .
- 注意事项
- Avoid repeated freeze-thaw cycles.
- 储存条件
- -80 °C
- 储存方法
- Store at -80°C.
- 有效期
- Unlimited (if stored properly)
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- 抗原
- PLOD1 (Procollagen-Lysine,2-Oxoglutarate 5-Dioxygenase 1 (PLOD1))
- 别名
- Procollagen-lysine,2-oxoglutarate 5-dioxygenase 1 (PLOD1 产品)
- 别名
- EDS6 Protein, LH Protein, LH1 Protein, LLH Protein, PLOD Protein, Plod Protein, procollagen-lysine Protein, 2410042F05Rik Protein, AI854890 Protein, AV007929 Protein, Lh1 Protein, plod1 Protein, ik:tdsubs_rzpd609-29b19 Protein, plod Protein, wu:fc29c10 Protein, wu:fc95g08 Protein, wu:fi18a05 Protein, zgc:152876 Protein, procollagen-lysine,2-oxoglutarate 5-dioxygenase 1 Protein, procollagen-lysine, 2-oxoglutarate 5-dioxygenase 1 Protein, procollagen-lysine,2-oxoglutarate 5-dioxygenase 3 Protein, procollagen-lysine, 2-oxoglutarate 5-dioxygenase 1 L homeolog Protein, procollagen-lysine, 2-oxoglutarate 5-dioxygenase 1a Protein, PLOD1 Protein, Plod1 Protein, PLOD3 Protein, plod1.L Protein, plod1a Protein
- UniProt
- G3IIE7
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